Combining H/D Exchange Mass Spectrometry and ComputationalDocking To Derive the Structure of Protein–Protein Complexes
Combining H/D Exchange Mass Spectrometry and ComputationalDocking To Derive the Structure of Protein–Protein Complexes
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00643/20171116/images/medium/bi-2017-00643w_0002.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00643
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11-20-2017 02:16 PM
[NMR paper] Insights into co-translational membrane protein insertion by combined LILBID-mass spectrometry and NMR spectroscopy.
Insights into co-translational membrane protein insertion by combined LILBID-mass spectrometry and NMR spectroscopy.
Related Articles Insights into co-translational membrane protein insertion by combined LILBID-mass spectrometry and NMR spectroscopy.
Anal Chem. 2017 Oct 17;:
Authors: Peetz O, Henrich E, Laguerre A, L?hr F, Hein C, Dötsch V, Bernhard F, Morgner N
Abstract
Co-translational insertion of membrane proteins into defined nanoparticle membranes has been developed as an efficient process to produce highly soluble samples...
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10-19-2017 10:47 PM
[NMR paper] Laser-initiated Radical Trifluoromethylation of Peptides and Proteins and Its Application to Mass Spectrometry-Based Protein Footprinting
Laser-initiated Radical Trifluoromethylation of Peptides and Proteins and Its Application to Mass Spectrometry-Based Protein Footprinting
We describe a novel, laser-initiated radical trifluoromethylation for protein footprinting and establish its broad residue coverage. *CF3 reacts with 18 of 20 common amino acids including Gly, Ala, Ser, Thr, Asp, Glu that are relatively "silent" with *OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to...
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09-13-2017 08:48 PM
Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
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Bioscience Technology
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Visualizing RNA-protein Interactions in Live Human Cells
Bioscience Technology
UAlbany chemists Alan Chen Ph.D, Maksim Royzen Ph.D, and Alex Shekhtman Ph.D have combined their labs on a collaborative project aimed at visualizing RNA-protein interactions inside live human cells by using multi-dimensional NMR (nuclear ...
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06-03-2017 11:49 AM
Characterizing the Structure and Oligomerization ofMajor Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and ComplementaryBiophysical Tools
Characterizing the Structure and Oligomerization ofMajor Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and ComplementaryBiophysical Tools
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00020/20170307/images/medium/bi-2017-000207_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00020
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03-08-2017 09:02 AM
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Publication date: Available online 27 December 2014
Source:Journal of Magnetic Resonance</br>
Author(s): M.-A. Sani , F. Separovic</br>
Understanding the structure of membrane-active peptides faces many challenges associated with the development of appropriate model membrane systems as the peptide structure depends strongly on the lipid environment. This perspective provides a brief overview of the approach taken to study antimicrobial and amyloid peptides in phospholipid...
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12-28-2014 10:29 PM
[NMR paper] Structure-based drug design: NMR-based approach for ligand-protein interactions.
Structure-based drug design: NMR-based approach for ligand-protein interactions.
Related Articles Structure-based drug design: NMR-based approach for ligand-protein interactions.
Drug Discov Today Technol. 2006;3(3):241-5
Authors: Zhang X, Tang H, Ye C, Liu M
Abstract
The realization of the powerfulness in analyzing ligand-protein interactions at the atomic resolution has made NMR techniques increasingly attractive in drug discovery and development. With some significant new method developments during the past few years,...
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07-06-2014 08:28 PM
[NMR paper] Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Chem Commun (Camb). 2013 Feb 26;
Authors: Takaoka Y, Kioi Y, Morito A, Otani J, Arita K, Ashihara E, Ariyoshi M, Tochio H, Shirakawa M, Hamachi I
Abstract
Here we describe how a (19)F-probe incorporated into an endogenous protein by a chemical biology method revealed protein dynamics. By explicit determination of ligand-bound and unbound structures with...
Membrane Protein Structure in Live Cells: Methodology for Studying Drug Interaction by Mass Spectrometry-Based Footprinting
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