NMR paper Measuring Phosphoglycolate Phosphatase Activity Using NMR Detection of Glycolate

		BioNMR > Educational resources > Journal club
[NMR paper] Measuring Phosphoglycolate Phosphatase Activity Using NMR Detection of Glycolate

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-12-2024, 01:00 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Measuring Phosphoglycolate Phosphatase Activity Using NMR Detection of Glycolate

Measuring Phosphoglycolate Phosphatase Activity Using NMR Detection of Glycolate

Besides the historical and traditional use of nuclear magnetic resonance (NMR) spectroscopy as a structure elucidation tool for proteins and metabolites, its quantification ability allows the determination of metabolite amounts and therefore enzymatic activity measurements. For this purpose, ¹H-NMR with adapted water pulse pre-saturation sequences and calibration curves with commercial standard solutions can be used to quantify the photorespiratory cycle intermediates, 2-phosphoglycolate and...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[ASAP] The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics https://pubs.acs.org/cms/10.1021/acs.biochem.2c00072/asset/images/medium/bi2c00072_0010.gif Biochemistry DOI: 10.1021/acs.biochem.2c00072 More...	nmrlearner	Journal club	0	05-11-2022 05:44 AM
[NMR paper] Perfluoro-tert-Butyl Hydroxyprolines as Sensitive, Conformationally Responsive Molecular Probes: Detection of Protein Kinase Activity by 19F NMR. Perfluoro-tert-Butyl Hydroxyprolines as Sensitive, Conformationally Responsive Molecular Probes: Detection of Protein Kinase Activity by 19F NMR. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Perfluoro-tert-Butyl Hydroxyprolines as Sensitive, Conformationally Responsive Molecular Probes: Detection of Protein Kinase Activity by 19F NMR. ACS Chem Biol. 2020 Mar 03;: Authors: Tressler CM, Zondlo NJ Abstract 19F NMR spectroscopy provides the ability to...	nmrlearner	Journal club	0	03-05-2020 08:33 AM
[ASAP] Facile, Fluorogenic Assay for Protein Histidine Phosphatase Activity Facile, Fluorogenic Assay for Protein Histidine Phosphatase Activity https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00278/20180423/images/medium/bi-2018-00278c_0004.gif Biochemistry DOI: 10.1021/acs.biochem.8b00278 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/XYxwyJU8rhA More...	nmrlearner	Journal club	0	04-24-2018 01:29 PM
[NMR paper] Enzyme responsive LipoCEST agents: assessment of MMP-2 activity by measuring the intra-liposomal water 1H-NMR resonance shift. Enzyme responsive LipoCEST agents: assessment of MMP-2 activity by measuring the intra-liposomal water 1H-NMR resonance shift. Related Articles Enzyme responsive LipoCEST agents: assessment of MMP-2 activity by measuring the intra-liposomal water 1H-NMR resonance shift. Angew Chem Int Ed Engl. 2017 Jul 26;: Authors: Ferrauto G, Di Gregorio E, Ruzza M, Catanzaro V, Padovan S, Aime S Abstract Mobile proton containing solutes can be detected by MRI via Chemical Exchange Saturation Transfer(CEST)method. The CEST sensitivity is...	nmrlearner	Journal club	0	07-27-2017 04:37 PM
[NMR paper] Enzyme responsive LipoCEST agents: assessment of MMP-2 activity by measuring the intra-liposomal water 1H-NMR resonance shift Enzyme responsive LipoCEST agents: assessment of MMP-2 activity by measuring the intra-liposomal water 1H-NMR resonance shift Mobile proton containing solutes can be detected by MRI via Chemical Exchange Saturation Transfer(CEST)method. The CEST sensitivity is dramatically enhanced by using, as exchanging protons, the water molecules confined inside liposomes, properly shifted by a paramagnetic Shift Reagent. The chemical shift of the intraliposomal water resonance(?IL) is affected by the overall shape of the supramolecular system. Herein, it is shown that ?IL of a spherical LipoCEST...	nmrlearner	Journal club	0	07-27-2017 01:04 AM
Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from ... - Nature.com Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from ... - Nature.com <img alt="" height="1" width="1"> Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from ... Nature.com The 1H and 13C NMR spectra (in acetone-d6) showed 23 proton and 16 carbon signals (Table 1) that were classified into one methyl, eight sp3 methylene, two sp3 oxygenated methine, two sp2 methine, two sp2 quaternary and one carbonyl carbons through ... Read here	nmrlearner	Online News	0	04-22-2015 03:33 PM
[NMR paper] Detection of fructose-3-phosphokinase activity in intact mammalian lenses by 31P NMR Detection of fructose-3-phosphokinase activity in intact mammalian lenses by 31P NMR spectroscopy. Related Articles Detection of fructose-3-phosphokinase activity in intact mammalian lenses by 31P NMR spectroscopy. J Biol Chem. 1993 Apr 15;268(11):7763-7 Authors: Lal S, Szwergold BS, Kappler F, Brown T Recently we have identified two novel phosphorylated metabolites in the lenses of diabetic rats as sorbitol 3-phosphate (Sor-3-P) and fructose 3-phosphate (Fru-3-P). The latter compound is of particular interest since it is a potent glycating...	nmrlearner	Journal club	0	08-21-2010 11:53 PM
[NMR paper] The detection of proline isomerase activity in FK506-binding protein by two-dimension The detection of proline isomerase activity in FK506-binding protein by two-dimensional 1H NMR exchange spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The detection of proline isomerase activity in FK506-binding protein by two-dimensional 1H NMR exchange spectroscopy. Biochem Biophys Res Commun. 1990 Aug 31;171(1):445-50 Authors: Justice RM, Kline AD, Sluka JP, Roeder WD, Rodgers GH, Roehm N, Mynderse JS 1H NMR assignments of the trans and cis isomers of...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off