Related ArticlesMass spectrometry assisted assignment of NMR resonances in 15N labeled proteins.
J Am Chem Soc. 2004 Nov 10;126(44):14377-9
Authors: Feng L, Orlando R, Prestegard JH
Application of nuclear magnetic resonance (NMR) methods for the structural characterization to larger and more complex protein systems can be facilitated through the development of new methods for resonance assignment. Here, a novel approach that relies on integration of NMR and mass spectrometry (MS) methods is explored. The approach relies on the fact that both NMR and MS are able to monitor rates of exchange of amide protons for water deuterons. Correlating the rates can connect cross-peak positions from NMR data with fragment masses from MS data to support sequential assignment. The example provided is for a small model protein, ubiquitin, but the potential for application to large, more difficult to express proteins is clear.
An intraresidual i(HCA)CO(CA)NH experiment for the assignment of main-chain resonances in 15N, 13C labeled proteins
An intraresidual i(HCA)CO(CA)NH experiment for the assignment of main-chain resonances in 15N, 13C labeled proteins
Abstract An improved pulse sequence, intraresidual i(HCA)CO(CA)NH, is described for establishing solely 13Câ?²(i), 15N(i), 1HN(i) connectivities in uniformly 15N/13C-labeled proteins. In comparison to the â??out-and-backâ?? style intra-HN(CA)CO experiment, the new pulse sequence offers at least two-fold higher experimental resolution in the 13Câ?² dimension and on average 1.6 times higher sensitivity especially for residues in α-helices. Performance of the new experiment...
nmrlearner
Journal club
0
01-09-2011 12:46 PM
[NMR paper] NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb
NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb and CDK6 with cell differentiation proteins MyoD and ID-2.
Related Articles NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb and CDK6 with cell differentiation proteins MyoD and ID-2.
Biochim Biophys Acta. 2005 Jun 15;1750(1):48-60
Authors: Smialowski P, Singh M, Mikolajka A, Majumdar S, Joy JK, Nalabothula N, Krajewski M, Degenkolbe R, Bernard HU, Holak TA
Cell growth and differentiation require precise coordination of...
nmrlearner
Journal club
0
11-25-2010 08:21 PM
[NMR paper] Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and (31)P-NMR.
Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and (31)P-NMR.
Related Articles Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and (31)P-NMR.
J Lipid Res. 2001 Sep;42(9):1501-8
Authors: Schiller J, Zschörnig O, Petkovi? M, Müller M, Arnhold J, Arnold K
The analysis of HDL and LDL is important for the further understanding of atherosclerosis because changes of the protein and lipid moieties occur under pathological conditions. Because destruction of lipids leads to the formation of well-defined products...
nmrlearner
Journal club
0
11-19-2010 08:44 PM
[NMR paper] Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spect
Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Related Articles Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Structure. 1994 Jun 15;2(6):465-7
Authors: Chait BT
nmrlearner
Journal club
0
08-22-2010 03:33 AM
[NMR paper] Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spect
Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Related Articles Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Structure. 1994 Jun 15;2(6):465-7
Authors: Chait BT
nmrlearner
Journal club
0
08-22-2010 03:33 AM
[NMR paper] Computer-assisted assignment of 2D 1H NMR spectra of proteins: basic algorithms and a
Computer-assisted assignment of 2D 1H NMR spectra of proteins: basic algorithms and application to phoratoxin B.
Related Articles Computer-assisted assignment of 2D 1H NMR spectra of proteins: basic algorithms and application to phoratoxin B.
J Biomol NMR. 1991 May;1(1):23-47
Authors: Kleywegt GJ, Boelens R, Cox M, Llinás M, Kaptein R
A suite of computer programs (CLAIRE) is described which can be of assistance in the process of assigning 2D 1H NMR spectra of proteins. The programs embody a software implementation of the sequential assignment...
nmrlearner
Journal club
0
08-21-2010 11:16 PM
Approaches to the assignment of 19F resonances from 3-fluorophenylalanine labeled cal
Abstract Traditional single site replacement mutations (in this case, phenylalanine to tyrosine) were compared with methods which exclusively employ 15N and 19F-edited two- and three-dimensional NMR experiments for purposes of assigning 19F NMR resonances from calmodulin (CaM), biosynthetically labeled with 3-fluorophenylalanine (3-FPhe). The global substitution of 3-FPhe for native phenylalanine was tolerated in CaM as evidenced by a comparison of 1H-15N HSQC spectra and calcium binding assays in the presence and absence of 3-FPhe. The 19F NMR spectrum reveals six resolved resonances, one...
nmrlearner
Journal club
0
08-14-2010 04:19 AM
Tenured Academic Staff - Mass spectrometry, University of Antwerp
I found the following job on spectroscopyNOW.com
Contact Name: Prof. Dr. R. Dommisse
Contact Email: Roger.Dommisse@ua.ac.be
Contact Telephone: +32 3 265 32 29
Employer: University of Antwerp
Background
Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins.
Linear Mode
