[NMR paper] Magnetically-orientable Tween-based model membranes for NMR studies of proteins.

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Magnetically-orientable Tween-based model membranes for NMR studies of proteins.

Related Articles Magnetically-orientable Tween-based model membranes for NMR studies of proteins.

Biochim Biophys Acta Biomembr. 2020 May 28;:183379

Authors: Gravel AE, Arnold AA, Fillion M, Auger M, Warschawski DE, Marcotte I

Abstract
We present a new membrane mimetic system using a membrane softening detergent commonly known as Tween 80 (TW80), to form oriented systems for solid-state NMR applications. TW80 is a fatty acid ester (oleate) of sorbitan polyethoxylate and a mild non-ionic surfactant. Phosphatidylcholine (PC)/TW80 model membrane systems were characterized by solid-state NMR and FTIR spectroscopy. 31P and 2H NMR spectra showed that DMPC (14:0) and DPPC (16:0) self-assemble with TW80 to form oriented structures, and maintain alignment over a wide range of molar ratios and temperatures. The addition of lanthanide ions revealed that the membrane alignment can be flipped from parallel to perpendicular with respect to the magnetic field direction. Using 15N solid-state NMR and a labeled model transmembrane peptide, we showed that TW80-based membranes can be employed to determine the peptide orientation in the magnetic field, which is useful for structural determination. Altogether, our work showed that TW80 could be exploited for direct and efficient membrane protein extraction and to enhance membrane and membrane protein orientation without using a detergent removal step. This approach could be extended to a wide range of membranes including native ones.


PMID: 32473826 [PubMed - as supplied by publisher]



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