Authors: Quinn CM, Lu M, Suiter CL, Hou G, Zhang H, Polenova T
Abstract
Viruses, relatively simple pathogens, are able to replicate in many living organisms and to adapt to various environments. Conventional atomic-resolution structural biology techniques, X-ray crystallography and solution NMR spectroscopy provided abundant information on the structures of individual proteins and nucleic acids comprising viruses; however, viral assemblies are not amenable to analysis by these techniques because of their large size, insolubility, and inherent lack of long-range order. In this article, we review the recent advances in magic angle spinning NMR spectroscopy that enabled atomic-resolution analysis of structure and dynamics of large viral systems and give examples of several exciting case studies.
PMID: 25919197 [PubMed - as supplied by publisher]
Magic angle spinning NMR of viruses
Magic angle spinning NMR of viruses
Publication date: April 2015
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volumes 86–87</br>
Author(s): Caitlin M. Quinn , Manman Lu , Christopher L. Suiter , Guangjin Hou , Huilan Zhang , Tatyana Polenova</br>
Viruses, relatively simple pathogens, are able to replicate in many living organisms and to adapt to various environments. Conventional atomic-resolution structural biology techniques, X-ray crystallography and solution NMR spectroscopy provided abundant information on the structures of individual...
nmrlearner
Journal club
0
04-12-2015 02:40 AM
[NMR paper] Pf1 bacteriophage hydration by magic angle spinning solid-state NMR.
Pf1 bacteriophage hydration by magic angle spinning solid-state NMR.
Pf1 bacteriophage hydration by magic angle spinning solid-state NMR.
J Chem Phys. 2014 Dec 14;141(22):22D533
Authors: Sergeyev IV, Bahri S, Day LA, McDermott AE
Abstract
High resolution two- and three-dimensional heteronuclear correlation spectroscopy ((1)H-(13)C, (1)H-(15)N, and (1)H-(13)C-(13)C HETCOR) has provided a detailed characterization of the internal and external hydration water of the Pf1 virion. This long and slender virion (2000 nm × 7 nm) contains...
nmrlearner
Journal club
0
12-17-2014 09:43 PM
Magic-Angle Spinning NMR of Cold Samples
From The DNP-NMR Blog:
Magic-Angle Spinning NMR of Cold Samples
Solid-state DNP experiments are often performed at cryogenic temperatures and this article is an excellent review about the current technologies to spin samples at (very) low temperatures.
ConcistrČ, M., et al., Magic-Angle Spinning NMR of Cold Samples. Acc. Chem. Res., 2013.
nmrlearner
News from NMR blogs
0
12-28-2013 06:42 AM
[NMR paper] Magic Angle Spinning NMR of Paramagnetic Proteins.
Magic Angle Spinning NMR of Paramagnetic Proteins.
Related Articles Magic Angle Spinning NMR of Paramagnetic Proteins.
Acc Chem Res. 2013 Mar 18;
Authors: Knight MJ, Felli IC, Pierattelli R, Emsley L, Pintacuda G
Abstract
Metal ions are ubiquitous in biochemical and cellular processes. Since many metal ions are paramagnetic due to the presence of unpaired electrons, paramagnetic molecules are an important class of targets for research in structural biology and related fields. Today, NMR spectroscopy plays a central role in the...
nmrlearner
Journal club
0
03-20-2013 01:47 PM
[NMR paper] Magic-Angle Spinning NMR of Cold Samples.
Magic-Angle Spinning NMR of Cold Samples.
Related Articles Magic-Angle Spinning NMR of Cold Samples.
Acc Chem Res. 2013 Mar 14;
Authors: Concistrč M, Johannessen OG, Carignani E, Geppi M, Levitt MH
Abstract
Magic-angle-spinning solid-state NMR provides site-resolved structural and chemical information about molecules that complements many other physical techniques. Recent technical advances have made it possible to perform magic-angle-spinning NMR experiments at low temperatures, allowing researchers to trap reaction intermediates and to...
nmrlearner
Journal club
0
03-16-2013 03:18 PM
[NMR paper] 13C magic angle spinning NMR characterization of the functionally asymmetric QA bindi
13C magic angle spinning NMR characterization of the functionally asymmetric QA binding in Rhodobacter sphaeroides R26 photosynthetic reaction centers using site-specific 13C-labeled ubiquinone-10.
Related Articles 13C magic angle spinning NMR characterization of the functionally asymmetric QA binding in Rhodobacter sphaeroides R26 photosynthetic reaction centers using site-specific 13C-labeled ubiquinone-10.
Biochemistry. 1995 Aug 15;34(32):10229-36
Authors: van Liemt WB, Boender GJ, Gast P, Hoff AJ, Lugtenburg J, de Groot HJ
Photosynthetic...
nmrlearner
Journal club
0
08-22-2010 03:50 AM
[NMR paper] 13C magic angle spinning NMR evidence for a 15,15'-cis configuration of the spheroide
13C magic angle spinning NMR evidence for a 15,15'-cis configuration of the spheroidene in the Rhodobacter sphaeroides photosynthetic reaction center.
Related Articles 13C magic angle spinning NMR evidence for a 15,15'-cis configuration of the spheroidene in the Rhodobacter sphaeroides photosynthetic reaction center.
Biochemistry. 1992 Dec 15;31(49):12446-50
Authors: de Groot HJ, Gebhard R, van der Hoef I, Hoff AJ, Lugtenburg J, Violette CA, Frank HA
The photosynthetic reaction center of Rhodobacter sphaeroides 2.4.1 contains one carotenoid...
nmrlearner
Journal club
0
08-21-2010 11:45 PM
[NMR paper] 13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of r
13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of rhodopsin.
Related Articles 13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of rhodopsin.
Biochemistry. 1991 Jul 30;30(30):7409-15
Authors: Smith SO, Courtin J, de Groot H, Gebhard R, Lugtenburg J
Magic-angle spinning NMR spectra have been obtained of the bathorhodopsin photointermediate trapped at low temperature (less than 130 K) by using isorhodopsin samples regenerated with retinal specifically 13C-labeled at positions 8,...
Magic angle spinning NMR of viruses.
Linear Mode
