Publication date: Available online 19 December 2017 Source:Current Opinion in Colloid & Interface Science
Author(s): Rupal Gupta, Tatyana Polenova
Since its discovery, human immunodeficiency virus (HIV) has been an intense subject of research. Several biophysical methodologies have aided towards understanding of the viral lifecycle and infectivity. Magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy has played a pivotal role in several key findings, providing atomistic details about the viral particles. These contributions have been made owing to tremendous advancements in NMR instrumentation, development of novel experiments and hybrid approaches combining NMR and computational methodologies. In this review, we highlight some advances made towards characterization of structure and dynamics in HIV-1 assemblies by MAS NMR spectroscopy. These findings have established MAS NMR spectroscopy is a promising methodology to investigate large biological assemblies such as viral particles and HIV-1 protein assemblies. Graphical abstract
[NMR paper] The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.
The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.
Related Articles The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.
Biochem Biophys Rep. 2016 Dec;8:75-80
Authors: Warner L, Gjersing E, Follett SE, Elliott KW, Dzyuba SV, Varga K
Abstract
Ionic liquids have great potential in biological applications and biocatalysis, as some...
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[NMR paper] Structural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy.
Structural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy.
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Sci Rep. 2017 Mar 17;7:44506
Authors: Yehl J, Kudryashova E, Reisler E, Kudryashov D, Polenova T
Abstract
Cellular actin dynamics is an essential element of numerous cellular processes, such as cell motility, cell division and...
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03-19-2017 10:38 PM
[NMR paper] Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
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Q Rev Biophys. 2017 Jan;50:e1
Authors: Quinn CM, Polenova T
Abstract
In recent years, exciting developments in instrument technology and experimental methodology have advanced the field of magic-angle spinning (MAS) nuclear magnetic resonance (NMR) to new heights. Contemporary MAS NMR yields atomic-level insights into structure and...
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01-19-2017 08:56 AM
The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy
The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy
Publication date: Available online 11 August 2016
Source:Biochemistry and Biophysics Reports</br>
Author(s): Lisa Warner, Erica Gjersing, Shelby E. Follett, K. Wade Elliott, Sergei V. Dzyuba, Krisztina Varga</br>
Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect....
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08-11-2016 07:03 PM
[NMR paper] Site-Resolved Backbone and Side-Chain Intermediate Dynamics in a Carbohydrate-Binding Module Protein Studied by Magic-Angle Spinning NMR Spectroscopy.
Site-Resolved Backbone and Side-Chain Intermediate Dynamics in a Carbohydrate-Binding Module Protein Studied by Magic-Angle Spinning NMR Spectroscopy.
Site-Resolved Backbone and Side-Chain Intermediate Dynamics in a Carbohydrate-Binding Module Protein Studied by Magic-Angle Spinning NMR Spectroscopy.
Chemistry. 2015 Jun 12;
Authors: Ivanir-Dabora H, Nimerovsky E, Madhu PK, Goldbourt A
Abstract
Magic-angle spinning solid-state NMR spectroscopy has been applied to study the dynamics of CBM3b-Cbh9A from Clostridium thermocellum...
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06-16-2015 07:24 PM
[NMR paper] Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
J Am Chem Soc. 2013 Oct 28;
Authors: Han Y, Hou G, Suiter CL, Ahn J, Byeon IJ, Lipton AS, Burton SD, Hung I, Gor'kov PL, Gan Z, Brey WW, Rice D, Gronenborn AM, Polenova TE
Abstract
A key stage in HIV-1 maturation towards...
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10-30-2013 10:44 AM
[NMR paper] Probing Structure and Dynamics of Protein Assemblies by Magic Angle Spinning NMR Spectroscopy.
Probing Structure and Dynamics of Protein Assemblies by Magic Angle Spinning NMR Spectroscopy.
Probing Structure and Dynamics of Protein Assemblies by Magic Angle Spinning NMR Spectroscopy.
Acc Chem Res. 2013 Feb 13;
Authors: Yan S, Suiter CL, Hou G, Zhang H, Polenova T
Abstract
In living organisms, biological molecules often organize into multicomponent complexes. Such assemblies consist of various proteins and carry out essential functions, ranging from cell division, transport, and energy transduction to catalysis, signaling, and viral...
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[NMR paper] Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
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J Am Chem Soc. 2005 Sep 21;127(37):12965-74
Authors: Andronesi OC, Becker S, Seidel K, Heise H, Young HS, Baldus M
It is shown that molecular structure and dynamics of a uniformly labeled membrane protein can be studied under magic-angle-spinning conditions. For this purpose, dipolar recoupling experiments...
Magic Angle Spinning NMR Spectroscopy Guided Atomistic Characterization of Structure and Dynamics in HIV-1 Protein Assemblies
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