Publication date: April 2015 Source:Journal of Magnetic Resonance, Volume 253
Author(s): Gili Abramov , Omry Morag , Amir Goldbourt
Bacteriophages are viruses that infect bacteria. They are complex macromolecular assemblies, which are composed of multiple protein subunits that protect genomic material and deliver it to specific hosts. Various biophysical techniques have been used to characterize their structure in order to unravel phage morphogenesis. Yet, most bacteriophages are non-crystalline and have very high molecular weights, in the order of tens of MegaDaltons. Therefore, complete atomic-resolution characterization on such systems that encompass both capsid and DNA is scarce. In this perspective article we demonstrate how magic-angle spinning solid-state NMR has and is used to characterize in detail bacteriophage viruses, including filamentous and icosahedral phage. We discuss the process of sample preparation, spectral assignment of both capsid and DNA and the use of chemical shifts and dipolar couplings to probe the capsid–DNA interface, describe capsid structure and dynamics and extract structural differences between viruses. Graphical abstract
Magic-Angle Spinning NMR of Cold Samples
From The DNP-NMR Blog:
Magic-Angle Spinning NMR of Cold Samples
Solid-state DNP experiments are often performed at cryogenic temperatures and this article is an excellent review about the current technologies to spin samples at (very) low temperatures.
ConcistrČ, M., et al., Magic-Angle Spinning NMR of Cold Samples. Acc. Chem. Res., 2013.
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http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja3004039/aop/images/medium/ja-2012-004039_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/ja3004039
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