Publication date: Available online 15 June 2018 Source:Biophysical Journal
Author(s): Jasmina Radoicic, Sang Ho Park, Stanley J. Opella
Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30*nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under physiological conditions. The narrow resonance linewidths observed from membrane proteins in styrene-maleic acid macrodiscs advance structure determination by oriented sample solid-state NMR spectroscopy.
[NMR paper] Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Related Articles Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Biophys J. 2014 Apr 15;106(8):1559-69
Authors: Murray DT, Li C, Gao FP, Qin H, Cross TA
Abstract
The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same...
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Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase
Publication date: 15 April 2014
Source:Biophysical Journal, Volume 106, Issue 8</br>
Author(s): Dylan*T. Murray , Conggang Li , F.*Philip Gao , Huajun Qin , Timothy*A. Cross</br>
The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same environment as that used for the structural analysis. This is difficult...
[NMR paper] Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Related Articles Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
J Biomol NMR. 2013 Dec 20;
Authors: Lu GJ, Opella SJ
Abstract
Oriented sample solid-state NMR spectroscopy can be used to determine the three-dimensional structures of membrane proteins in magnetically or mechanically aligned lipid bilayers. The...
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[NMR paper] Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
Related Articles Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
J Phys Chem B. 2013 Sep 17;
Authors: Das BB, Lin EC, Opella SJ
Abstract
Structure determination by solid-state NMR of proteins is rapidly advancing as result of recent developments of samples, experimental methods, and calculations. There are a number of different solid-state NMR approaches that utilize stationary, aligned samples or...
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins
Linear Mode
