BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search
Home Forums Wiki NMR feeds Downloads Register Today's Posts



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
 
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Old 09-12-2011, 09:00 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,732
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Low-field one-dimensional and direction-dependent relaxation imaging of bovine articular cartilage

Low-field one-dimensional and direction-dependent relaxation imaging of bovine articular cartilage


Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 10 September 2011

Erik*Rössler, Carlos*Mattea, Ayret*Mollova, Siegfried*Stapf

The structure of articular cartilage is separated into three layers of differently oriented collagen fibers, which is accompanied by a gradient of increasing glycosaminoglycan (GAG) and decreasing water concentration from the top layer towards the bone interface. The combined effect of these structural variations results in a change of the longitudinal and transverse relaxation times as a function of the distance from the cartilage surface. In this paper, this dependence is investigated at a magnetic field strength of 0.27 T with a one-dimensional depth resolution of 50 ?m on bovine hip and stifle joint articular cartilage. By employing this method, advantage is taken of the increasing contrast of the longitudinal relaxation rate found at lower magnetic field strengths. Furthermore, evidence for an orientational dependence of relaxation times with respect to an axis normal to the surface plane is given, an observation that has recently been reported using high-field MRI and that was explained by preferential orientations of collagen bundles in each of the three cartilage zones. In order to quantify the extent of a further contrast mechanism and to estimate spatially dependent glycosaminoglycan concentrations, the data are supplemented by proton relaxation times that were acquired in bovine articular cartilage that was soaked in a 0.8mM aqueous Gdsolution.

Graphical abstract



Highlights

? Bovine articular cartilage was investigated by low-field 1D MRI at 50 ?m resolution. ? Relaxation times T1, T2 reveal internal cartilage structure. ? Strong T1 variations are found, in contrast to high-field studies. ? Contrast agents allow the estimation of local GAG (protein) concentrations. ? Angular dependence of T2 indicates preferred collagen fiber orientation.



Source: Journal of Magnetic Resonance
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 23 April 2011</br> Xiao-Hong, Zhu , Wei, Chen</br> *Highlights:*? This article reviews the developments of in vivo 17O NMR imaging in brain research. ? In vivo 17O NMR imaging has improved significantly at high/ultrahigh field. ? In vivo 17O NMR can noninvasively image brain oxygen metabolism and perfusion. ? In vivo 17O NMR is useful for mapping the functional change in oxygen...
nmrlearner Journal club 0 04-24-2011 03:42 PM
[NMR paper] Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and
Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and line-shape analysis. Related Articles Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and line-shape analysis. Biopolymers. 2000 Jan;53(1):9-18 Authors: Mack JW, Usha MG, Long J, Griffin RG, Wittebort RJ We have used 2H-nmr to study backbone dynamics of the 2H-labeled, slowly exchanging amide sites of fully hydrated, crystalline hen egg white lysozyme. Order parameters are determined from the residual quadrupole coupling and...
nmrlearner Journal club 0 11-18-2010 09:15 PM
[NMR paper] 600 MHz NMR studies of human articular cartilage keratan sulfates.
600 MHz NMR studies of human articular cartilage keratan sulfates. Related Articles 600 MHz NMR studies of human articular cartilage keratan sulfates. Eur J Biochem. 1999 Dec;266(3):1174-83 Authors: Huckerby TN, Nieduszynski IA, Bayliss MT, Brown GM The use of high-field two-dimensional 1H-correlation data is described for the detailed comparison of intact keratan sulfate polymer chains derived from human articular cartilage sources as a function of age. For fetal material the nonreducing chain termini are shown to be sparsely capped by sialyl...
nmrlearner Journal club 0 11-18-2010 08:31 PM
[NMR tweet] www.sciencia.org Development of Ultra Low Field Nuclear Magnetic Resonance Imaging Sy
www.sciencia.org Development of Ultra Low Field Nuclear Magnetic Resonance Imaging System Using HTS rf SQUID http://dlvr.it/77T6B Published by PhysicsSci (Sciencia Physics) on 2010-10-17T08:47:06Z Source: Twitter
nmrlearner Twitter NMR 0 10-17-2010 09:08 AM
[NMR paper] A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-associa
A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association. Biochemistry. 1997 Mar 18;36(11):3383-8 Authors: Ilyina E, Roongta V, Pan H, Woodward C, Mayo KH Previous studies have produced conflicting interpretations regarding the aggregation state of BPTI in solution. Here, pulsed-field gradient NMR self-association...
nmrlearner Journal club 0 08-22-2010 03:31 PM
[NMR paper] A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-associa
A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association. Biochemistry. 1997 Mar 18;36(11):3383-8 Authors: Ilyina E, Roongta V, Pan H, Woodward C, Mayo KH Previous studies have produced conflicting interpretations regarding the aggregation state of BPTI in solution. Here, pulsed-field gradient NMR self-association...
nmrlearner Journal club 0 08-22-2010 03:03 PM
[NMR paper] Three-dimensional structure of bovine heart fatty-acid-binding protein with bound pal
Three-dimensional structure of bovine heart fatty-acid-binding protein with bound palmitic acid, determined by multidimensional NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Three-dimensional structure of bovine heart fatty-acid-binding protein with bound palmitic acid, determined by multidimensional NMR spectroscopy. Eur J Biochem. 1995 May 15;230(1):266-80 Authors: Lassen D, Lücke C, Kveder M, Mesgarzadeh A, Schmidt JM,...
nmrlearner Journal club 0 08-22-2010 03:41 AM
[NMR paper] The three-dimensional structure of acyl-coenzyme A binding protein from bovine liver:
The three-dimensional structure of acyl-coenzyme A binding protein from bovine liver: structural refinement using heteronuclear multidimensional NMR spectroscopy. Related Articles The three-dimensional structure of acyl-coenzyme A binding protein from bovine liver: structural refinement using heteronuclear multidimensional NMR spectroscopy. J Biomol NMR. 1993 May;3(3):271-84 Authors: Andersen KV, Poulsen FM The 3D structure of bovine recombinant acyl-coenzyme A binding protein has been determined using multidimensional heteronuclear magnetic...
nmrlearner Journal club 0 08-21-2010 11:53 PM



Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 04:57 AM.


Map