Publication year: 2011 Source: Journal of Magnetic Resonance, Available online 10 September 2011
Erik*Rössler, Carlos*Mattea, Ayret*Mollova, Siegfried*Stapf
The structure of articular cartilage is separated into three layers of differently oriented collagen fibers, which is accompanied by a gradient of increasing glycosaminoglycan (GAG) and decreasing water concentration from the top layer towards the bone interface. The combined effect of these structural variations results in a change of the longitudinal and transverse relaxation times as a function of the distance from the cartilage surface. In this paper, this dependence is investigated at a magnetic field strength of 0.27 T with a one-dimensional depth resolution of 50 ?m on bovine hip and stifle joint articular cartilage. By employing this method, advantage is taken of the increasing contrast of the longitudinal relaxation rate found at lower magnetic field strengths. Furthermore, evidence for an orientational dependence of relaxation times with respect to an axis normal to the surface plane is given, an observation that has recently been reported using high-field MRI and that was explained by preferential orientations of collagen bundles in each of the three cartilage zones. In order to quantify the extent of a further contrast mechanism and to estimate spatially dependent glycosaminoglycan concentrations, the data are supplemented by proton relaxation times that were acquired in bovine articular cartilage that was soaked in a 0.8mM aqueous Gdsolution. Graphical abstract
Highlights
? Bovine articular cartilage was investigated by low-field 1D MRI at 50 ?m resolution. ? Relaxation times T1, T2 reveal internal cartilage structure. ? Strong T1 variations are found, in contrast to high-field studies. ? Contrast agents allow the estimation of local GAG (protein) concentrations. ? Angular dependence of T2 indicates preferred collagen fiber orientation.
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 23 April 2011</br>
Xiao-Hong, Zhu , Wei, Chen</br>
*Highlights:*? This article reviews the developments of in vivo 17O NMR imaging in brain research. ? In vivo 17O NMR imaging has improved significantly at high/ultrahigh field. ? In vivo 17O NMR can noninvasively image brain oxygen metabolism and perfusion. ? In vivo 17O NMR is useful for mapping the functional change in oxygen...
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[NMR paper] Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and
Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and line-shape analysis.
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Biopolymers. 2000 Jan;53(1):9-18
Authors: Mack JW, Usha MG, Long J, Griffin RG, Wittebort RJ
We have used 2H-nmr to study backbone dynamics of the 2H-labeled, slowly exchanging amide sites of fully hydrated, crystalline hen egg white lysozyme. Order parameters are determined from the residual quadrupole coupling and...
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[NMR paper] 600 MHz NMR studies of human articular cartilage keratan sulfates.
600 MHz NMR studies of human articular cartilage keratan sulfates.
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Eur J Biochem. 1999 Dec;266(3):1174-83
Authors: Huckerby TN, Nieduszynski IA, Bayliss MT, Brown GM
The use of high-field two-dimensional 1H-correlation data is described for the detailed comparison of intact keratan sulfate polymer chains derived from human articular cartilage sources as a function of age. For fetal material the nonreducing chain termini are shown to be sparsely capped by sialyl...
[NMR paper] A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-associa
A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
Biochemistry. 1997 Mar 18;36(11):3383-8
Authors: Ilyina E, Roongta V, Pan H, Woodward C, Mayo KH
Previous studies have produced conflicting interpretations regarding the aggregation state of BPTI in solution. Here, pulsed-field gradient NMR self-association...
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[NMR paper] A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-associa
A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
Biochemistry. 1997 Mar 18;36(11):3383-8
Authors: Ilyina E, Roongta V, Pan H, Woodward C, Mayo KH
Previous studies have produced conflicting interpretations regarding the aggregation state of BPTI in solution. Here, pulsed-field gradient NMR self-association...
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[NMR paper] Three-dimensional structure of bovine heart fatty-acid-binding protein with bound pal
Three-dimensional structure of bovine heart fatty-acid-binding protein with bound palmitic acid, determined by multidimensional NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Three-dimensional structure of bovine heart fatty-acid-binding protein with bound palmitic acid, determined by multidimensional NMR spectroscopy.
Eur J Biochem. 1995 May 15;230(1):266-80
Authors: Lassen D, Lücke C, Kveder M, Mesgarzadeh A, Schmidt JM,...
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[NMR paper] The three-dimensional structure of acyl-coenzyme A binding protein from bovine liver:
The three-dimensional structure of acyl-coenzyme A binding protein from bovine liver: structural refinement using heteronuclear multidimensional NMR spectroscopy.
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J Biomol NMR. 1993 May;3(3):271-84
Authors: Andersen KV, Poulsen FM
The 3D structure of bovine recombinant acyl-coenzyme A binding protein has been determined using multidimensional heteronuclear magnetic...