NMR paper Low-field NMR works as a rapid, automatic, non-invasive and wide-scale coverage technique for aggregates indication in biomacromolecule development

		BioNMR > Educational resources > Journal club
[NMR paper] Low-field NMR works as a rapid, automatic, non-invasive and wide-scale coverage technique for aggregates indication in biomacromolecule development

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-05-2024, 09:35 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Low-field NMR works as a rapid, automatic, non-invasive and wide-scale coverage technique for aggregates indication in biomacromolecule development

Low-field NMR works as a rapid, automatic, non-invasive and wide-scale coverage technique for aggregates indication in biomacromolecule development

Protein aggregation is challenging for biopharmaceutical drug, because it affects the stability of protein formulations in real-time. However, current techniques for protein aggregate indication meet a number of limitations including limited aggregate size range, complex pre-treatments and lack of chromatographic approaches. Herein, a rapid, automatic, non-invasive and wide-scale coverage technique for aggregates indication is developed to overcome these challenges. Firstly, the response of...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA - Nature.com Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA - Nature.com Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA Nature.com Read here	nmrlearner	Online News	0	08-01-2024 05:54 PM
Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA - Nature.com Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA - Nature.com Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA Nature.com Read here	nmrlearner	Online News	0	12-28-2023 10:50 PM
[NMR paper] Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA Nuclear Magnetic Resonance (NMR) spectroscopy is a major technique in structural biology with over 11,800 protein structures deposited in the Protein Data Bank. NMR can elucidate structures and dynamics of small and medium size proteins in solution, living cells, and solids, but has been limited by the tedious data analysis process. It typically requires weeks or months of manual work of a trained expert to turn NMR measurements into a protein structure. Automation of this process is an...	nmrlearner	Journal club	0	10-20-2022 06:37 PM
[NMR paper] Rapid NMR-scale purification of 15N,13C isotope-labeled recombinant human STIM1 coiled coil fragments. Rapid NMR-scale purification of 15N,13C isotope-labeled recombinant human STIM1 coiled coil fragments. Rapid NMR-scale purification of 15N,13C isotope-labeled recombinant human STIM1 coiled coil fragments. Protein Expr Purif. 2018 Feb 01;146:45-50 Authors: Rathner P, Stadlbauer M, Romanin C, Fahrner M, Derler I, Müller N Abstract We report a new NMR-scale purification procedure for two recombinant wild type fragments of the stromal interaction molecule 1 (STIM1). This protein acts as a calcium sensor in the endoplasmic reticulum...	nmrlearner	Journal club	0	02-08-2018 04:32 PM
[NMR paper] Development of a low resolution (1)H NMR spectroscopic technique for the study of matrix mobility in fresh and freeze-thawed hen egg yolk. Development of a low resolution (1)H NMR spectroscopic technique for the study of matrix mobility in fresh and freeze-thawed hen egg yolk. Development of a low resolution (1)H NMR spectroscopic technique for the study of matrix mobility in fresh and freeze-thawed hen egg yolk. Food Chem. 2016 Aug 1;204:159-66 Authors: Au C, Wang T, Acevedo NC Abstract	nmrlearner	Journal club	0	03-19-2016 09:23 PM
[NMR paper] Solution NMR structures provide first structural coverage of the large protein domain family PF08369 and complementary structural coverage of dark operative protochlorophyllide oxidoreductase complexes. Solution NMR structures provide first structural coverage of the large protein domain family PF08369 and complementary structural coverage of dark operative protochlorophyllide oxidoreductase complexes. Solution NMR structures provide first structural coverage of the large protein domain family PF08369 and complementary structural coverage of dark operative protochlorophyllide oxidoreductase complexes. J Struct Funct Genomics. 2013 Aug 21; Authors: Pulavarti SV, He Y, Feldmann EA, Eletsky A, Acton TB, Xiao R, Everett JK, Montelione GT, Kennedy MA, Szyperski...	nmrlearner	Journal club	0	08-23-2013 01:07 AM
[NMR paper] Rapid sample-mixing technique for transient NMR and photo-CIDNP spectroscopy: applica Rapid sample-mixing technique for transient NMR and photo-CIDNP spectroscopy: applications to real-time protein folding. Related Articles Rapid sample-mixing technique for transient NMR and photo-CIDNP spectroscopy: applications to real-time protein folding. J Am Chem Soc. 2003 Oct 15;125(41):12484-92 Authors: Mok KH, Nagashima T, Day IJ, Jones JA, Jones CJ, Dobson CM, Hore PJ We describe the development and application of a novel rapid sample-mixing technique for real-time NMR (nuclear magnetic resonance) spectroscopy. The apparatus consists...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
SAGA: rapid automatic mainchain NMR assignment for large proteins Abstract Here we describe a new algorithm for automatically determining the mainchain sequential assignment of NMR spectra for proteins. Using only the customary triple resonance experiments, assignments can be quickly found for not only small proteins having rather complete data, but also for large proteins, even when only half the residues can be assigned. The result of the calculation is not the single best assignment according to some criterion, but rather a large number of satisfactory assignments that are summarized in such a way as to help the user identify portions of the sequence...	nmrlearner	Journal club	0	08-14-2010 04:19 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off