Abstract Longitudinal exchange experiments facilitate the quantification of the rates of interconversion between the exchanging species, along with their longitudinal relaxation rates, by analyzing the time-dependence of direct correlation and exchange cross peaks. Here we present a simple and robust alternative to this strategy, which is based on the combination of two complementary experiments, one with and one without resolving exchange cross peaks. We show that by combining the two data sets systematic errors that are caused by differential line-broadening of the exchanging species are avoided and reliable quantification of kinetic and relaxation parameters in the presence of additional conformational exchange on the msâ??μs time scale is possible. The strategy is applied to a bistable DNA oligomer that displays different line-broadening in the two exchanging species.
Content Type Journal Article
Category Article
Pages 123-129
DOI 10.1007/s10858-011-9547-8
Authors
Karin Kloiber, Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020 Innsbruck, Austria
Romana Spitzer, Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020 Innsbruck, Austria
Sarina Grutsch, Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020 Innsbruck, Austria
Christoph Kreutz, Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020 Innsbruck, Austria
Martin Tollinger, Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020 Innsbruck, Austria
[Question from NMRWiki Q&A forum] Chemical exchange of Tyr residues - what information can be found about dynamics?
Chemical exchange of Tyr residues - what information can be found about dynamics?
I have an aromatic spectrum of Tyr residues in p53 DNA binding domain. No peaks are seen for Tyr-205 at high temp, but as temp decreases 4 peaks are shown. Tyr-163 and Tyr-236 show two peak each at high temps. How come there aren't 4 peaks? The dynamic processes are said to occur on different time scales. What is the interpretation of this information? How is the core domain of p53 influenced by this? Thanks for your help!
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04-11-2011 12:48 AM
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Quantification of protein backbone hydrogen-deuterium exchange rates by solid state N
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Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy
Linear Mode
