NMR paper Long-observation-window band-selective homonuclear decoupling: Increased sensitivity and resolution in solid-state NMR spectroscopy of proteins.

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[NMR paper] Long-observation-window band-selective homonuclear decoupling: Increased sensitivity and resolution in solid-state NMR spectroscopy of proteins.

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10-08-2013, 02:04 PM

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Long-observation-window band-selective homonuclear decoupling: Increased sensitivity and resolution in solid-state NMR spectroscopy of proteins.

Long-observation-window band-selective homonuclear decoupling: Increased sensitivity and resolution in solid-state NMR spectroscopy of proteins.

Long-observation-window band-selective homonuclear decoupling: Increased sensitivity and resolution in solid-state NMR spectroscopy of proteins.

J Magn Reson. 2013 Sep 13;236C:89-94

Authors: Struppe JO, Yang C, Wang Y, Hernandez RV, Shamansky LM, Mueller LJ

Abstract
Sensitivity and resolution are the two fundamental obstacles to extending solid-state nuclear magnetic resonance to even larger protein systems. Here, a novel long-observation-window band-selective homonuclear decoupling (LOW BASHD) scheme is introduced that increases resolution up to a factor of 3 and sensitivity up to 1.8 by decoupling backbone alpha-carbon (C(?)) and carbonyl (C') nuclei in U-(13)C-labeled proteins during direct (13)C acquisition. This approach introduces short (

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