NMR paper The location of the polyphosphate-binding sites on cytochrome c measured by NMR param

		BioNMR > Educational resources > Journal club
[NMR paper] The location of the polyphosphate-binding sites on cytochrome c measured by NMR param

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:12 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

The location of the polyphosphate-binding sites on cytochrome c measured by NMR param

The location of the polyphosphate-binding sites on cytochrome c measured by NMR paramagnetic difference spectroscopy.

Related Articles The location of the polyphosphate-binding sites on cytochrome c measured by NMR paramagnetic difference spectroscopy.

Eur J Biochem. 1991 Aug 1;199(3):569-74

Authors: Concar DW, Whitford D, Williams RJ

Analyses of unimolecular electron self-exchange reactions provide a comparatively simple and direct approach to understanding biological electron transfer. Such studies are currently limited by a lack of well characterised aggregating systems. In the presence of sodium hexametaphosphate, cytochrome c forms stable protein aggregates as a result of binding hexametaphosphate at a single site on its surface (preceding paper in this issue of the journal). Here we report the location of the principal polyphosphate binding site on the surface of cytochrome c for both hexametaphosphate and a second polyphosphate, tripolyphosphate determined using 1H-NMR spectroscopy in conjunction with the relaxation probe potassium hexacyanochromium(III). Addition of either hexametaphosphate or tripolyphosphate to ferricytochrome c in the presence of the relaxation probe causes a decrease in intensity of several resonances in the paramagnetic difference spectrum, including Phe82 ortho/meta, Ile85 delta methyl and Ile9 gamma methyl. Together these effects put the site of polyphosphate binding close to lysines 13, 86, and 87. Additionally the effect of sodium tripolyphosphate and sodium trimetaphosphate on cytochrome c aggregation is described. The potential role of this site in anion-induced cytochrome c aggregation is discussed.

PMID: 1651238 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Location of the Zn(2+)-binding site on S100B as determined by NMR spectroscopy and si Location of the Zn(2+)-binding site on S100B as determined by NMR spectroscopy and site-directed mutagenesis. Related Articles Location of the Zn(2+)-binding site on S100B as determined by NMR spectroscopy and site-directed mutagenesis. Biochemistry. 2003 Nov 25;42(46):13410-21 Authors: Wilder PT, Baldisseri DM, Udan R, Vallely KM, Weber DJ In addition to binding Ca(2+), the S100 protein S100B binds Zn(2+) with relatively high affinity as confirmed using isothermal titration calorimetry (ITC; K(d) = 94 +/- 17 nM). The Zn(2+)-binding site on...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Protein hydration and location of water molecules in oxidized horse heart cytochrome Protein hydration and location of water molecules in oxidized horse heart cytochrome c by (1)H NMR. Related Articles Protein hydration and location of water molecules in oxidized horse heart cytochrome c by (1)H NMR. J Magn Reson. 2000 Nov;147(1):1-8 Authors: Bertini I, Huber JG, Luchinat C, Piccioli M The hydration properties of the oxidized form of horse heart cytochrome c have been studied by (1)H NMR spectroscopy. Two-dimensional, homonuclear ePHOGSY-NOESY experiments are used to map water-protein interactions. The detected NOEs reveal...	nmrlearner	Journal club	0	11-19-2010 08:29 PM
[NMR paper] 19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP recept 19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP receptor protein from E. coli. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP receptor protein from E. coli. FEBS Lett. 1991 May 20;283(1):127-30 Authors: Hinds MG, King RW, Feeney J The 19F NMR spectra of 3-fluorotyrosine containing c-AMP receptor protein (CRP) from E. coli have been recorded in the presence of...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR paper] The location of the polyphosphate-binding sites on cytochrome c measured by NMR param The location of the polyphosphate-binding sites on cytochrome c measured by NMR paramagnetic difference spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles The location of the polyphosphate-binding sites on cytochrome c measured by NMR paramagnetic difference spectroscopy. Eur J Biochem. 1991 Aug 1;199(3):569-74 Authors: Concar DW, Whitford D, Williams RJ Analyses of unimolecular electron self-exchange reactions provide...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] 19F NMR study of the myosin and tropomyosin binding sites on actin. 19F NMR study of the myosin and tropomyosin binding sites on actin. Related Articles 19F NMR study of the myosin and tropomyosin binding sites on actin. Biochemistry. 1990 Feb 6;29(5):1348-54 Authors: Barden JA, Phillips L Actin was labeled with pentafluorophenyl isothiocyanate at Lys-61. The label was sufficiently small not to affect the rate or extent of actin polymerization unlike the much larger fluorescein 5-isothiocyanate which completely inhibits actin polymerization . Furthermore, the label resonances in the 376.3-MHz 19F NMR spectrum...	nmrlearner	Journal club	0	08-21-2010 10:48 PM
[NMR paper] Location of a cation-binding site in the loop between helices F and G of bacteriorhod Location of a cation-binding site in the loop between helices F and G of bacteriorhodopsin as studied by 13C NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Location of a cation-binding site in the loop between helices F and G of bacteriorhodopsin as studied by 13C NMR. Biophys J. 1999 Mar;76(3):1523-31 Authors: Tuzi S, Yamaguchi S, Tanio M, Konishi H, Inoue S, Naito A,...	nmrlearner	Journal club	0	08-21-2010 04:03 PM
[NMR paper] Identification of the ribosome binding sites of translation initiation factor IF3 by Identification of the ribosome binding sites of translation initiation factor IF3 by multidimensional heteronuclear NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Identification of the ribosome binding sites of translation initiation factor IF3 by multidimensional heteronuclear NMR spectroscopy. RNA. 1999 Jan;5(1):82-92 Authors: Sette M, Spurio R, van Tilborg P, Gualerzi CO, Boelens R Titrations of Escherichia coli translation initiation...	nmrlearner	Journal club	0	08-21-2010 04:03 PM
Prediction of binding sites from protein structure Surfnet http://www.biochem.ucl.ac.uk/~roman/surfnet/surfnet.html	nmrlearner	A test forum	0	09-19-2005 05:54 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off