Location of a cation-binding site in the loop between helices F and G of bacteriorhodopsin as studied by 13C NMR.
Biophys J. 1999 Mar;76(3):1523-31
Authors: Tuzi S, Yamaguchi S, Tanio M, Konishi H, Inoue S, Naito A, Needleman R, Lanyi JK, Saitô H
The high-affinity cation-binding sites of bacteriorhodopsin (bR) were examined by solid-state 13C NMR of samples labeled with [3-13C]Ala and [1-13C]Val. We found that the 13C NMR spectra of two kinds of blue membranes, deionized (pH 4) and acid blue at pH 1.2, were very similar and different from that of the native purple membrane. This suggested that when the surface pH is lowered, either by removal of cations or by lowering the bulk pH, substantial change is induced in the secondary structure of the protein. Partial replacement of the bound cations with Na+, Ca2+, or Mn2+ produced additional spectral changes in the 13C NMR spectra. The following conclusions were made. First, there are high-affinity cation-binding sites in both the extracellular and the cytoplasmic regions, presumably near the surface, and one of the preferred cation-binding sites is located at the loop between the helix F and G (F-G loop) near Ala196, consistent with the 3D structure of bR from x-ray diffraction and cryoelectron microscopy. Second, the bound cations undergo rather rapid exchange (with a lifetime shorter than 3 ms) among various types of cation-binding sites. As expected from the location of one of the binding sites, cation binding induced conformational alteration of the F-G interhelical loop.
[NMR paper] NMR conformational analyses on (des-bromo) neuropeptide B [1-23] and neuropeptide W [1-23]: the importance of alpha-helices, a cation-pi interaction and a beta-turn.
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J Biomol Struct Dyn. 2005 Aug;23(1):77-90
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The preferred conformations of the orphan G-protein coupled receptor agonists (des-bromo) neuropeptide B and neuropeptide W , referred to...
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[NMR paper] Location of the Zn(2+)-binding site on S100B as determined by NMR spectroscopy and si
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Biochemistry. 2003 Nov 25;42(46):13410-21
Authors: Wilder PT, Baldisseri DM, Udan R, Vallely KM, Weber DJ
In addition to binding Ca(2+), the S100 protein S100B binds Zn(2+) with relatively high affinity as confirmed using isothermal titration calorimetry (ITC; K(d) = 94 +/- 17 nM). The Zn(2+)-binding site on...
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[NMR paper] NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop bin
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RNA. 2002 Jan;8(1):83-96
Authors: DeJong ES, Marzluff WF, Nikonowicz EP
The 3' end of replication-dependent histone mRNAs terminate in a conserved sequence containing a stem-loop. This 26-nt sequence is the binding site for a protein, stem-loop binding protein (SLBP), that is involved in multiple aspects of histone mRNA metabolism...
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[NMR paper] Solution-state NMR investigations of triosephosphate isomerase active site loop motio
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J Mol Biol. 2001 Jun 29;310(1):271-80
Authors: Rozovsky S, Jogl G, Tong L, McDermott AE
Product release is partially rate determining in the isomerization reaction catalyzed by Triosephosphate Isomerase, the conversion of dihydroxyacetone...
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Biochemistry. 2000 Aug 22;39(33):10066-71
Authors: Helmle M, Patzelt H, Ockenfels A, Gärtner W, Oesterhelt D, Bechinger B
The bacterial proton pump bacteriorhodopsin (BR) is a 26.5 kDa seven-transmembrane helical protein. Several...
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[NMR paper] The identification of cation-binding domains on the surface of microsomal cytochrome
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Eur J Biochem. 1992 Jan 15;203(1-2):211-23
Authors: Whitford D
One-dimensional and two-dimensional 1H-NMR...
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[NMR paper] The location of the polyphosphate-binding sites on cytochrome c measured by NMR param
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Eur J Biochem. 1991 Aug 1;199(3):569-74
Authors: Concar DW, Whitford D, Williams RJ
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[NMR paper] The location of the polyphosphate-binding sites on cytochrome c measured by NMR param
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Eur J Biochem. 1991 Aug 1;199(3):569-74
Authors: Concar DW, Whitford D, Williams RJ
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