Authors: Shapira B, Lupulescu A, Shrot Y, Frydman L
We have recently proposed and demonstrated an approach that enables the acquisition of 2D nuclear magnetic resonance (NMR) spectra within a single scan. The approach is based on spatially encoding the spins' evolution along the indirect domain with the aid of a magnetic field gradient, and subsequently decoding this information numerous times over the course of the signal acquisition while spins are subject to a train of gradient echoes. The present paper discusses further considerations pertaining the 2D line shapes arising from this new way of collecting NMR data. Specific issues that are hereby addressed include (i) the effects introduced by fast relaxation onto the spatial encoding process, particularly the line widths and line shapes that will then arise in the frequency domain; (ii) approaches capable of correcting for the mixed-phase kernels resulting in these fast-relaxation cases, corresponding in essence to spatially encoded analogs of the TPPI and hypercomplex time-domain acquisition procedures; (iii) the enveloping characteristics imposed by the use of discrete excitation pulses on the attainable spectral widths along the indirect domain; and (iv) an analysis of the signal-to-noise characteristics of the methodology, with experimental corroborations of theoretical predictions and an illustration of the method's capabilities to analyze protein solutions in the mM-range concentration.
[Question from NMRWiki Q&A forum] EBURP-2 wavelib shape pulse file for varian
EBURP-2 wavelib shape pulse file for varian
Can somebody please send the EBURP-2 wavelib file for VNMR?
Thanks!
AL
nmrlearner
News from other NMR forums
0
11-06-2011 10:24 PM
Compressed sensing and the reconstruction of ultrafast 2D NMR data: Principles and biomolecular applications.
Compressed sensing and the reconstruction of ultrafast 2D NMR data: Principles and biomolecular applications.
Compressed sensing and the reconstruction of ultrafast 2D NMR data: Principles and biomolecular applications.
J Magn Reson. 2011 Apr;209(2):352-8
Authors: Shrot Y, Frydman L
A topic of active investigation in 2D NMR relates to the minimum number of scans required for acquiring this kind of spectra, particularly when these are dictated by sampling rather than by sensitivity considerations. Reductions in this minimum number of scans have...
nmrlearner
Journal club
0
07-23-2011 08:54 AM
Peak shape in NMR
Hi there,
I'm quite new to NMR, but one thing I've been wondering is: are the shapes of the peaks in an NMR spectrum important? I know that the frequency information is important, and the amplitude can sometimes be important for experiments like NOESY, but does the overall shape matter? Can anymore information be deduced from this?
Thanks
newToNMR
NMR Questions and Answers
1
12-13-2010 06:50 AM
[NMR paper] Sensitivity improvement in 19F NMR-based screening experiments: theoretical considerations and experimental applications.
Sensitivity improvement in 19F NMR-based screening experiments: theoretical considerations and experimental applications.
Related Articles Sensitivity improvement in 19F NMR-based screening experiments: theoretical considerations and experimental applications.
J Am Chem Soc. 2005 Sep 28;127(38):13380-5
Authors: Dalvit C, Mongelli N, Papeo G, Giordano P, Veronesi M, Moskau D, Kümmerle R
NMR-based binding and functional screening performed with FAXS (fluorine chemical shift anisotropy and exchange for screening) and 3-FABS (three fluorine atoms...
nmrlearner
Journal club
0
12-01-2010 06:56 PM
[NMR paper] Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interac
Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interactions.
Related Articles Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interactions.
J Magn Reson. 2004 Nov;171(1):163-70
Authors: Shrot Y, Shapira B, Frydman L
A new protocol for acquiring multidimensional NMR spectra within a single scan is introduced and illustrated. The approach relies on applying a pair of frequency-chirped excitation and storage pulses in combination with echoing magnetic field gradients, in order to impart...
nmrlearner
Journal club
0
11-24-2010 10:03 PM
[NMR paper] Dynamic NMR line-shape analysis demonstrates that the villin headpiece subdomain fold
Dynamic NMR line-shape analysis demonstrates that the villin headpiece subdomain folds on the microsecond time scale.
Related Articles Dynamic NMR line-shape analysis demonstrates that the villin headpiece subdomain folds on the microsecond time scale.
J Am Chem Soc. 2003 May 21;125(20):6032-3
Authors: Wang M, Tang Y, Sato S, Vugmeyster L, McKnight CJ, Raleigh DP
There is considerable interest in small proteins that fold very rapidly. These proteins have become attractive targets for both theoretical and computational studies. The...
Protein alignment using cellulose nanocrystals: practical considerations and range of
Abstract Cellulose nanocrystals (CNCs) form liquid crystals in aqueous solution that confer alignment to macromolecules and permit the measurement of residual dipolar couplings. CNCs possess many attractive features as an alignment medium. They are inexpensive, non-toxic, chemically inert, and robust to denaturants and temperature. Despite these advantages, CNCs are seldom employed as an alignment medium and the range of their applicability has not yet been explored. We have re-examined the use of CNCs in biomolecular NMR by analyzing the effects concentration, ionic strength, and...
Line shape considerations in ultrafast 2D NMR.
Linear Mode
