In structural studies by NMR, pseudocontact shifts (PCSs) provide both angular and distance information. For proteins, incorporation of a di-histidine (diHis) motif, coordinated to Co^(2+), has emerged as an important tool to measure PCS. Here, we show that using different Co(II)-chelating ligands, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), resolves the isosurface ambiguity of Co^(2+)-diHis and yields orthogonal PCS data sets with different ??-tensors for the same...
[NMR paper] The Cu2+-nitrilotriacetic acid complex improves loading of ?-helical double histidine site for precise distance measurements by pulsed ESR
The Cu2+-nitrilotriacetic acid complex improves loading of ?-helical double histidine site for precise distance measurements by pulsed ESR
Publication date: Available online 7 December 2017
Source:Journal of Magnetic Resonance</br>
Author(s): Shreya Ghosh, Matthew J. Lawless, Gordon S. Rule, Sunil Saxena</br>
Site-directed spin labeling using two strategically placed natural histidine residues allows for the rigid attachment of paramagnetic Cu2+. This double histidine (dHis) motif enables extremely precise, narrow distance distributions resolved by Cu2+-based...
[NMR paper] NMR studies of the phosphorylation motif of the HIV-1 protein Vpu bound to the F-box
NMR studies of the phosphorylation motif of the HIV-1 protein Vpu bound to the F-box protein beta-TrCP.
Related Articles NMR studies of the phosphorylation motif of the HIV-1 protein Vpu bound to the F-box protein beta-TrCP.
Biochemistry. 2003 Dec 23;42(50):14741-51
Authors: Coadou G, Gharbi-Benarous J, Megy S, Bertho G, Evrard-Todeschi N, Segeral E, Benarous R, Girault JP
A protein-protein association regulated by phosphorylation of serine is examined by NMR studies. Degradation of the HIV receptor CD4 by the proteasome, mediated by the HIV-1...
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[NMR paper] NMR studies of metal ion binding to the Zn-finger-like HNH motif of colicin E9.
NMR studies of metal ion binding to the Zn-finger-like HNH motif of colicin E9.
Related Articles NMR studies of metal ion binding to the Zn-finger-like HNH motif of colicin E9.
J Inorg Biochem. 2000 Apr;79(1-4):365-70
Authors: Hannan JP, Whittaker SB, Hemmings AM, James R, Kleanthous C, Moore GR
The 134 amino acid DNase domain of colicin E9 contains a zinc-finger-like HNH motif that binds divalent transition metal ions. We have used 1D 1H and 2D 1H-15N NMR methods to characterise the binding of Co2+, Ni2+ and Zn2+ to this protein. Data for the...
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[NMR paper] Heteronuclear NMR studies of cobalt corrinoids. 20. 31P chemical shift anisotropy of
Heteronuclear NMR studies of cobalt corrinoids. 20. 31P chemical shift anisotropy of aquacobalamin and its complex with a haptocorrin from chicken serum.
Related Articles Heteronuclear NMR studies of cobalt corrinoids. 20. 31P chemical shift anisotropy of aquacobalamin and its complex with a haptocorrin from chicken serum.
J Inorg Biochem. 1998 Sep;71(3-4):199-204
Authors: Brown KL, Wilson WW, Jacobsen DW
Static light scattering measurements have been used to determine the molecular mass (65.3 kDa) and second virial coefficient (3.66 x 10(-4)...
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[NMR paper] NMR studies of tandem WW domains of Nedd4 in complex with a PY motif-containing regio
NMR studies of tandem WW domains of Nedd4 in complex with a PY motif-containing region of the epithelial sodium channel.
Related Articles NMR studies of tandem WW domains of Nedd4 in complex with a PY motif-containing region of the epithelial sodium channel.
Biochem Cell Biol. 1998;76(2-3):341-50
Authors: Kanelis V, Farrow NA, Kay LE, Rotin D, Forman-Kay JD
Nedd4 (neuronal precursor cell-expressed developmentally down-regulated 4) is a ubiquitin-protein ligase containing multiple WW domains. We have previously demonstrated the association...
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[NMR paper] Architectural rules of the zinc-finger motif: comparative two-dimensional NMR studies
Architectural rules of the zinc-finger motif: comparative two-dimensional NMR studies of native and "aromatic-swap" domains define a "weakly polar switch".
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Architectural rules of the zinc-finger motif: comparative two-dimensional NMR studies of native and "aromatic-swap" domains define a "weakly polar switch".
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8455-9
Authors: Kochoyan M, Keutmann HT, Weiss MA
The...