[NMR paper] Latent Biochemical Relationships in the Blood-Milk Metabolic Axis of Dairy Cows Revealed by Statistical Integration of (1)H NMR Spectroscopic Data.
Latent Biochemical Relationships in the Blood-Milk Metabolic Axis of Dairy Cows Revealed by Statistical Integration of (1)H NMR Spectroscopic Data.
J Proteome Res. 2013 Feb 8;
Authors: Maher AD, Hayes B, Cocks B, Marett L, Wales WJ, Rochfort S
Abstract
A detailed understanding of the relationships between the distinct metabolic compartments of blood and milk would be of potential benefit to our understanding of the physiology of lactation, and potentially for development of biomarkers for health and commercially-relevant traits in dairy cattle. NMR methods were used to measure metabolic profiles from blood and milk samples from Holstein cows. Data were analyzed using PLS regression to identify quantitative relationships between metabolic profiles and important traits. Statistical Heterospectroscopy (SHY), a powerful approach to recovering latent biological information in NMR spectroscopic datasets from multiple complementary samples, was employed to explore the metabolic relationships between blood and milk from these animals. The study confirms milk is a distinct metabolic compartment with a metabolite composition largely not influenced by plasma composition under normal circumstances. However, several significant relationships were identified, including a high correlation for trimethylamine (TMA) and dimethylsulfone (DMSO(2)) across plasma and milk compartments, and evidence plasma valine levels are linked to differences in amino acid catabolism in the mammary gland. The findings provide insights into the physiological mechanisms underlying lactation and identification of links between key metabolites and milk traits such as the protein and fat content of milk. The approach has the potential to enable measurement of health, metabolic status and other important phenotypes with milk sampling.
PMID: 23394630 [PubMed - as supplied by publisher]
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
J Proteome Res. 2010 Dec 3;9(12):6729-39
Authors: Szeto SS, Reinke SN, Sykes BD, Lemire BD
Metabolomics is a powerful method of examining the intricate connections between mutations, metabolism, and disease. Metabolic...
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Chembiochem. 2010 Nov 22;11(17):2424-32
Authors: Díaz MD, Palomino-Schätzlein M, Corzana F, Andreu C, Carbajo RJ, del Olmo M, Canales-Mayordomo A, Pineda-Lucena A, Asensio G, Jiménez-Barbero J
The conformations of two synthetic pentapeptides with...
nmrlearner
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05-04-2011 04:14 PM
CONNJUR - open source integration environment for biomolecular NMR data analysis
CONNJUR Project website
The CONNJUR Project is developing an open source integration environment for biomolecular NMR data analysis. CONNJUR software is a workflow generator, based on legacy NMR analysis applications, that is being developed as Open Source Software -- it is perpetually free for anyone to use, modify and distribute.
CONNJUR is developed by a community of NMR spectroscopists and scientific programmers who aim to create and maintain NMR analysis tools bounded by the goals of excellent end-product, free of charge, open source software using the...
markber
NMR software
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11-01-2010 07:45 PM
[NMR paper] A biochemical and NMR spectroscopic study of hydrazine in the isolated rat hepatocyte
A biochemical and NMR spectroscopic study of hydrazine in the isolated rat hepatocyte.
Related Articles A biochemical and NMR spectroscopic study of hydrazine in the isolated rat hepatocyte.
Arch Toxicol. 1992;66(9):660-8
Authors: Ghatineh S, Morgan W, Preece NE, Timbrell JA
Using isolated rat hepatocytes the biochemical effects of hydrazine have been investigated using both conventional assay techniques and high resolution proton NMR. High resolution proton NMR revealed that hydrazine caused a significant increase in alanine and lactate...
Latent Biochemical Relationships in the Blood-Milk Metabolic Axis of Dairy Cows Revealed by Statistical Integration of (1)H NMR Spectroscopic Data.
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