NMR paper Lasso Proteins: Modular Design, Cellular Synthesis and Topological Transformation

		BioNMR > Educational resources > Journal club
[NMR paper] Lasso Proteins: Modular Design, Cellular Synthesis and Topological Transformation

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-01-2020, 07:32 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Lasso Proteins: Modular Design, Cellular Synthesis and Topological Transformation

Lasso Proteins: Modular Design, Cellular Synthesis and Topological Transformation

Angewandte Chemie International Edition, Accepted Article.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR. Methods Enzymol. 2017;592:49-76 Authors: Thompson MK, Ehlinger AC, Chazin WJ Abstract Multiprotein machines drive virtually all primary cellular processes. Modular multidomain proteins are widely distributed within these dynamic complexes because they provide the flexibility needed to remodel structure as well as rapidly assemble and disassemble components of the...	nmrlearner	Journal club	0	07-03-2017 03:18 PM
Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR Publication date: Available online 17 April 2017 Source:Methods in Enzymology</br> Author(s): Matthew K. Thompson, Aaron C. Ehlinger, Walter J. Chazin</br> Multiprotein machines drive virtually all primary cellular processes. Modular multidomain proteins are widely distributed within these dynamic complexes because they provide the flexibility needed to remodel structure as well as rapidly assemble and disassemble components of the machinery. Understanding the functional dynamics of...	nmrlearner	Journal club	0	05-10-2017 06:52 AM
[NMR paper] Design, synthesis, biological evaluation, NMR and DFT studies of structurally-simplified trimethoxy benzamides as P-glycoprotein selective inhibitors: the role of molecular flatness. Design, synthesis, biological evaluation, NMR and DFT studies of structurally-simplified trimethoxy benzamides as P-glycoprotein selective inhibitors: the role of molecular flatness. Design, synthesis, biological evaluation, NMR and DFT studies of structurally-simplified trimethoxy benzamides as P-glycoprotein selective inhibitors: the role of molecular flatness. Chem Biol Drug Des. 2016 Jun 22; Authors: Stefanachi A, Mangiatordi GF, Tardia P, Alberga D, Leonetti F, Niso M, Colabufo NA, Adamo C, Nicolotti O, Cellamare S Abstract ...	nmrlearner	Journal club	0	06-23-2016 10:34 AM
[NMR paper] Rational design and synthesis of an orally bioavailable peptide guided by NMR amide temperature coefficients. Rational design and synthesis of an orally bioavailable peptide guided by NMR amide temperature coefficients. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full.gif Related Articles Rational design and synthesis of an orally bioavailable peptide guided by NMR amide temperature coefficients. Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):17504-9 Authors: Wang CK, Northfield SE, Colless B, Chaousis S, Hamernig I, Lohman RJ, Nielsen DS, Schroeder CI, Liras S, Price...	nmrlearner	Journal club	0	04-29-2015 03:49 PM
Towarda Biorelevant Structure of Protein Kinase CBound Modulators: Design, Synthesis, and Evaluation of Labeled BryostatinAnalogues for Analysis with Rotational Echo Double Resonance NMR Spectroscopy Towarda Biorelevant Structure of Protein Kinase CBound Modulators: Design, Synthesis, and Evaluation of Labeled BryostatinAnalogues for Analysis with Rotational Echo Double Resonance NMR Spectroscopy Brian A. Loy, Adam B. Lesser, Daryl Staveness, Kelvin L. Billingsley, Lynette Cegelski and Paul A. Wender http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b00886/20150304/images/medium/ja-2015-00886q_0011.gif Journal of the American Chemical Society DOI: 10.1021/jacs.5b00886 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	03-04-2015 08:57 PM
[NMR paper] Toward a Biorelevant Structure of Protein Kinase C Bound Modulators: Design, Synthesis, and Evaluation of Labeled Bryostatin Analogs for Analysis with REDOR NMR Spectroscopy. Toward a Biorelevant Structure of Protein Kinase C Bound Modulators: Design, Synthesis, and Evaluation of Labeled Bryostatin Analogs for Analysis with REDOR NMR Spectroscopy. Toward a Biorelevant Structure of Protein Kinase C Bound Modulators: Design, Synthesis, and Evaluation of Labeled Bryostatin Analogs for Analysis with REDOR NMR Spectroscopy. J Am Chem Soc. 2015 Feb 24; Authors: Loy BA, Lesser AB, Staveness D, Billingsley KL, Cegelski L, Wender PA Abstract Protein kinase C (PKC) modulators are currently of great...	nmrlearner	Journal club	0	02-25-2015 05:56 PM
[NMR paper] Design, synthesis, structural characterization by IR, (1) H, (13) C, (15) N, 2D-NMR, X-ray diffraction and evaluation of a new class of phenylaminoacetic acid benzylidene hydrazines as pfENR inhibitors. Design, synthesis, structural characterization by IR, (1) H, (13) C, (15) N, 2D-NMR, X-ray diffraction and evaluation of a new class of phenylaminoacetic acid benzylidene hydrazines as pfENR inhibitors. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Design, synthesis, structural characterization by IR, (1) H, (13) C, (15) N, 2D-NMR, X-ray diffraction and evaluation of a new class of phenylaminoacetic acid benzylidene hydrazines as pfENR inhibitors. ...	nmrlearner	Journal club	0	01-04-2014 01:39 AM
Oligo design tools for gene synthesis Oligo design tools for gene synthesis 1 DNAWorks: http://helixweb.nih.gov/dnaworks/ 2 TmPrime: http://prime.ibn.a-star.edu.sg/	nmrlearner	Proteins	0	08-25-2010 04:37 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off