[NMR paper] Lanthanide Tagging of Oligonucleotides to Nucleobase for Paramagnetic NMR.

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Lanthanide Tagging of Oligonucleotides to Nucleobase for Paramagnetic NMR.

Related Articles Lanthanide Tagging of Oligonucleotides to Nucleobase for Paramagnetic NMR.

Chembiochem. 2020 Jul 20;:

Authors: Täubert S, Zhang YH, Maestre-Martinez M, Siepel F, Wöltjen E, Leonov A, Griesinger C

Abstract
Although lanthanide tags, which have large anisotropic magnetic susceptibilities, have already been introduced to enrich the NMR parameters by long ranging pseudoconact shifts (PCSs) and residual dipolar couplings (RDCs) of proteins, their application to nucleotides has so far been limited to one previous report, due to the high affinities of lanthanides to the phosphodiester backbone of nucleotides and difficult organic synthesis. Here we report successful attachment of a lanthanide tag to a chemically synthesized oligonucleotide via a disulfide bond. NMR experiments reveal PCSs up to 1 ppm and H-H RDCs up to 8 Hz at 950 MHz. Although* weaker magnetic alignment was achieved compared to proteins, the paramagnetic data could be fitted to the known structure of the DNA taking the mobility of the tag into account. While further rigidification of the tag is desirable, this tag could also be used to measure heteronuclear RDCs of 13 C, 15 N labeled chemically synthesized DNA and RNA.


PMID: 32687667 [PubMed - as supplied by publisher]



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