NMR paper Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR a

		BioNMR > Educational resources > Journal club
[NMR paper] Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR a

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 02:27 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR a

Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.

Related Articles Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.

Anticancer Res. 1996 May-Jun;16(3B):1375-80

Authors: Katz-Brull R, Degani H

The mechanism and kinetics of choline transport and phosphorylation in MCF7 human breast cancer cells was studied by 31P, 13C and 2H NMR, applying the zero trans method. Choline was transported by a Michaelis-Menten like mechanism with a maximum transport rate T(max) = 13.5 +/- 2.6 nmol/hour/mg protein (3.06 +/- 0.6 fmol/cell/hour) and choline concentration at half maximal transport rate of Kt = 46.5 +/- 2.8 microM. The rate of choline phosphorylation was more than two orders of magnitude faster than the rate of its transport (T(max)) maintaining the ratio [phosphocholine]/[choline] higher than 100. The results demonstrated enhanced choline transport and choline kinase activity in breast cancer cells.

PMID: 8694504 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system 1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system Abstract Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics technologies are capable of simultaneously measuring a wide range of metabolites in an untargeted manner, these methods have been relatively under utilized in the study of cancer cell responses to hypoxia. Thus, 1H NMR metabolomics was...	nmrlearner	Journal club	0	03-09-2011 04:19 AM
[NMR tweet] For Women With Breast Cancer, It's Buyer Beware: NMR (nuclear magnetic resonance) scan of woman showing cancer i... http://bit.ly/fS5trE For Women With Breast Cancer, It's Buyer Beware: NMR (nuclear magnetic resonance) scan of woman showing cancer i... http://bit.ly/fS5trE Published by Cancer4breast (Sue Stock-Cojocaru) on 2011-01-04T02:22:58Z Source: Twitter	nmrlearner	Twitter NMR	0	01-04-2011 02:29 AM
[NMR paper] 13C/31P NMR studies on the role of glucose transport/phosphorylation in human glycoge 13C/31P NMR studies on the role of glucose transport/phosphorylation in human glycogen supercompensation. Related Articles 13C/31P NMR studies on the role of glucose transport/phosphorylation in human glycogen supercompensation. Int J Sports Med. 2003 May;24(4):238-44 Authors: Price TB, Laurent D, Petersen KF This study measured muscle glycogen during a 7-day carbohydrate loading protocol. Twenty healthy subjects (12 male, 8 female) performed 1 hr treadmill/toe-raise exercise immediately before a 3-day low carbohydrate (LoCHO) diet (20 %...	nmrlearner	Journal club	0	11-24-2010 09:01 PM
[NMR tweet] Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Screening: As e Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Screening: As explained in the previous arti... http://bit.ly/bcqLcs Published by soni2006 (Rajinder Soni) on 2010-10-16T05:39:39Z Source: Twitter	nmrlearner	Twitter NMR	0	10-16-2010 05:58 AM
[NMR tweet] Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Screening: As e Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Screening: As explained in the previous arti... http://bit.ly/bcqLcs Published by soni2006 (Rajinder Soni) on 2010-10-16T05:39:39Z Source: Twitter	nmrlearner	Twitter NMR	0	10-16-2010 05:58 AM
[NMR tweet] #hubpages Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Scree #hubpages Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Screening http://bit.ly/bcqLcs Published by Q2A4U (Question_Answer) on 2010-10-16T04:49:29Z Source: Twitter	nmrlearner	Twitter NMR	0	10-16-2010 05:58 AM
[NMR tweet] Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Screening http Use of Nuclear Magnetic Resonance and Breast Biopsy for Breast Cancer Screening http://ping.fm/eCNX2 Published by soni2006 (Rajinder Soni) on 2010-10-16T04:14:14Z Source: Twitter	nmrlearner	Twitter NMR	0	10-16-2010 04:47 AM
Biochemical disorders induced by cytotoxic marine natural products in breast cancer c Biochemical disorders induced by cytotoxic marine natural products in breast cancer cells as revealed by proton NMR spectroscopy-based metabolomics. Related Articles Biochemical disorders induced by cytotoxic marine natural products in breast cancer cells as revealed by proton NMR spectroscopy-based metabolomics. Biochem Pharmacol. 2010 Oct 15;80(8):1170-9 Authors: Bayet-Robert M, Lim S, Barthomeuf C, Morvan D Marine plants and animals are sources of a huge number of pharmacologically active compounds, some of which exhibit antineoplastic...	nmrlearner	Journal club	0	10-12-2010 02:52 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off