Related ArticlesKinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.
Anticancer Res. 1996 May-Jun;16(3B):1375-80
Authors: Katz-Brull R, Degani H
The mechanism and kinetics of choline transport and phosphorylation in MCF7 human breast cancer cells was studied by 31P, 13C and 2H NMR, applying the zero trans method. Choline was transported by a Michaelis-Menten like mechanism with a maximum transport rate T(max) = 13.5 +/- 2.6 nmol/hour/mg protein (3.06 +/- 0.6 fmol/cell/hour) and choline concentration at half maximal transport rate of Kt = 46.5 +/- 2.8 microM. The rate of choline phosphorylation was more than two orders of magnitude faster than the rate of its transport (T(max)) maintaining the ratio [phosphocholine]/[choline] higher than 100. The results demonstrated enhanced choline transport and choline kinase activity in breast cancer cells.
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
Abstract Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics technologies are capable of simultaneously measuring a wide range of metabolites in an untargeted manner, these methods have been relatively under utilized in the study of cancer cell responses to hypoxia. Thus, 1H NMR metabolomics was...
[NMR paper] 13C/31P NMR studies on the role of glucose transport/phosphorylation in human glycoge
13C/31P NMR studies on the role of glucose transport/phosphorylation in human glycogen supercompensation.
Related Articles 13C/31P NMR studies on the role of glucose transport/phosphorylation in human glycogen supercompensation.
Int J Sports Med. 2003 May;24(4):238-44
Authors: Price TB, Laurent D, Petersen KF
This study measured muscle glycogen during a 7-day carbohydrate loading protocol. Twenty healthy subjects (12 male, 8 female) performed 1 hr treadmill/toe-raise exercise immediately before a 3-day low carbohydrate (LoCHO) diet (20 %...
Biochemical disorders induced by cytotoxic marine natural products in breast cancer c
Biochemical disorders induced by cytotoxic marine natural products in breast cancer cells as revealed by proton NMR spectroscopy-based metabolomics.
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Biochem Pharmacol. 2010 Oct 15;80(8):1170-9
Authors: Bayet-Robert M, Lim S, Barthomeuf C, Morvan D
Marine plants and animals are sources of a huge number of pharmacologically active compounds, some of which exhibit antineoplastic...