RNA is central to the proper function of cellular processes important for life on earth and implicated in various medical dysfunctions. Yet, RNA structural biology lags significantly behind that of proteins, limiting mechanistic understanding of RNA chemical biology. Fortunately, solution NMR spectroscopy can probe the structural dynamics of RNA in solution at atomic resolution, opening the door to their functional understanding. However, NMR analysis of RNA, with only four unique ribonucleotide...
[NMR paper] Application of NMR Spectroscopy to Determine the 3D Structure of Small Non-Coding RNAs
Application of NMR Spectroscopy to Determine the 3D Structure of Small Non-Coding RNAs
Many RNA architectures were discovered to be involved in a wide range of essential biological processes in all organisms from carrying genetic information to gene expression regulation. The remarkable ability of RNAs to adopt various architectures depending on their environment enables the achievement of their myriads of biological functions. Nuclear Magnetic Resonance (NMR) is a powerful technique to investigate both their structure and dynamics. NMR is also a key tool for studying interactions...
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04-03-2021 10:01 PM
NMR probing of invisible excited states using selectively labeled RNAs
NMR probing of invisible excited states using selectively labeled RNAs
Abstract
Carrâ??Purcellâ??Meiboomâ??Gill (CPMG) relaxation dispersion NMR experiments are invaluable for probing sparsely and transiently populated biomolecular states that cannot be directly detected by traditional NMR experiments and that are invisible by other biophysical approaches. A notable gap for RNA is the absence of CPMG experiments for measurement of methine base 1H and methylene C5â?² chemical shifts of ribose moieties in the excited state, partly because of...
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06-02-2018 12:51 AM
[ASAP] Accurate Measurement of Residual Dipolar Couplings in Large RNAs by Variable Flip Angle NMR
Accurate Measurement of Residual Dipolar Couplings in Large RNAs by Variable Flip Angle NMR
Jan Marchant, Ad Bax, Michael F. Summers
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b03298/20180525/images/medium/ja-2018-03298s_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.8b03298
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/mk_Vi4slk10
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05-26-2018 01:57 AM
Evaluation of 15 N-detected Hâ??N correlation experiments on increasingly large RNAs
Evaluation of 15 N-detected Hâ??N correlation experiments on increasingly large RNAs
Abstract
Recently, 15N-detected multidimensional NMR experiments have been introduced for the investigation of proteins. Utilization of the slow transverse relaxation of nitrogen nuclei in a 15N-TROSY experiment allowed recording of high quality spectra for high molecular weight proteins, even in the absence of deuteration. Here, we demonstrate the applicability of three 15N-detected Hâ??N correlation experiments (TROSY, BEST-TROSY and HSQC) to RNA. With the newly...
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09-07-2017 04:35 AM
19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy
19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy
Abstract
In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted chemical shift dispersion in only four different nucleotides spectral resolution remains limited in larger RNAs. Labeling RNAs with the NMR-active nucleus 19F has previously been introduced for small RNAs up to 40...
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12-28-2015 12:26 AM
[NMR paper] (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
(19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
Related Articles (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
J Biomol NMR. 2015 Dec 24;
Authors: Sochor F, Silvers R, Müller D, Richter C, Fürtig B, Schwalbe H
Abstract
In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted...
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12-28-2015 12:26 AM
[NMR paper] Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution.
Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution.
Related Articles Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution.
Methods Enzymol. 2015;558:279-331
Authors: Duss O, Yulikov M, Allain FH, Jeschke G
Abstract
Although functional significance of large noncoding RNAs and their complexes with proteins is well recognized, structural information for this class of systems is very scarce. Their inherent flexibility causes problems in...
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06-13-2015 11:09 PM
Solvent and H/D Isotope Effects on the Proton TransferPathways in Heteroconjugated Hydrogen-Bonded Phenol-Carboxylic AcidAnions Observed by Combined UVvis and NMR Spectroscopy
Solvent and H/D Isotope Effects on the Proton TransferPathways in Heteroconjugated Hydrogen-Bonded Phenol-Carboxylic AcidAnions Observed by Combined UVvis and NMR Spectroscopy
Benjamin Koeppe, Jing Guo, Peter M. Tolstoy, Gleb S. Denisov and Hans-Heinrich Limbach
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja400611x/aop/images/medium/ja-2013-00611x_0015.gif
Journal of the American Chemical Society
DOI: 10.1021/ja400611x
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
Isotope Labels Combined with Solution NMR Spectroscopy Make Visible the Invisible Conformations of Small-to-Large RNAs
Linear Mode
