NMR paper Investigation of relative metabolic changes in the organs and plasma of rats exposed to X-ray radiation using HR-MAS (1) H NMR and solution (1) H NMR.

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[NMR paper] Investigation of relative metabolic changes in the organs and plasma of rats exposed to X-ray radiation using HR-MAS (1) H NMR and solution (1) H NMR.

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02-13-2016, 02:40 PM

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Investigation of relative metabolic changes in the organs and plasma of rats exposed to X-ray radiation using HR-MAS (1) H NMR and solution (1) H NMR.

Investigation of relative metabolic changes in the organs and plasma of rats exposed to X-ray radiation using HR-MAS (1) H NMR and solution (1) H NMR.

Related Articles Investigation of relative metabolic changes in the organs and plasma of rats exposed to X-ray radiation using HR-MAS (1) H NMR and solution (1) H NMR.

NMR Biomed. 2016 Feb 12;

Authors: Jang WG, Park JY, Lee J, Bang E, Kim SR, Lee EK, Yun HJ, Kang CM, Hwang GS

Abstract
Excess exposure to ionizing radiation generates reactive oxygen species and increases the cellular inflammatory response by modifying various metabolic pathways. However, an investigation of metabolic perturbations and organ-specific responses based on the amount of radiation during the acute phase has not been conducted. In this study, high-resolution magic-angle-spinning (HR-MAS) NMR and solution NMR-based metabolic profiling were used to investigate dose-dependent metabolic changes in multiple organs and tissues - including the jejunum, spleen, liver, and plasma - of rats exposed to X-ray radiation. The organs, tissues, and blood samples were obtained 24, 48, and 72 h after exposure to low-dose (2 Gy) and high-dose (6 Gy) X-ray radiation and subjected to metabolite profiling and multivariate analyses. The results showed the time course of the metabolic responses, and many significant changes were detected in the high-dose compared with the low-dose group. Metabolites with antioxidant properties showed acute responses in the jejunum and spleen after radiation exposure. The levels of metabolites related to lipid and protein metabolism were decreased in the jejunum. In addition, amino acid levels increased consistently at all post-irradiation time points as a consequence of activated protein breakdown. Consistent with these changes, plasma levels of tricarboxylic acid cycle intermediate metabolites decreased. The liver did not appear to undergo remarkable metabolic changes after radiation exposure. These results may provide insight into the major metabolic perturbations and mechanisms of the biological systems in response to pathophysiological damage caused by X-ray radiation. Copyright © 2016 John Wiley & Sons, Ltd.

PMID: 26871685 [PubMed - as supplied by publisher]

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