Related ArticlesInvestigation of proteins in living bacteria with in-cell NMR experiments.
Top Curr Chem. 2008;273:203-14
Authors: Dötsch V
Abstract
In recent years NMR methods have been developed that enable the observation of proteins insideliving bacterial cells. Because of the sensitivity of the chemical shift to environmental changesthese in-cell NMR experiments can be used to study protein conformation, molecular interaction ordynamics in a*protein's natural surrounding. Detection of proteins in the bacterial cytoplasmrelies on labeling of the protein of interest with NMR active isotopes. This review describes differentlabeling techniques based on either uniform (15)N or (13)Clabeling as well as amino acid specific labeling schemes. In addition potential applications of thesein-cell NMR experiments and their limitations are discussed.
[NMR paper] Protein dynamics in living cells studied by in-cell NMR spectroscopy.
Protein dynamics in living cells studied by in-cell NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein dynamics in living cells studied by in-cell NMR spectroscopy.
FEBS Lett. 2013 Jan 11;
Authors: Li C, Liu M
Abstract
Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques...
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Protein dynamics in living cells studied by in-cell NMR spectroscopy
Protein dynamics in living cells studied by in-cell NMR spectroscopy
Available online 11 January 2013
Publication year: 2013
Source:FEBS Letters</br>
</br>
Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques can provide protein structure and other biophysical properties inside living cells at atomic resolution. Here we review how protein dynamics, including global and internal motions have been...
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02-03-2013 10:13 AM
On-Cell MAS NMR: PhysiologicalClues from Living Cells
On-Cell MAS NMR: PhysiologicalClues from Living Cells
Giorgia Zandomeneghi, Karin Ilg, Markus Aebi and Beat H. Meier
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja307467p/aop/images/medium/ja-2012-07467p_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/ja307467p
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/PBl2HVolQkY
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[NMR paper] Methyl groups as probes for proteins and complexes in in-cell NMR experiments.
Methyl groups as probes for proteins and complexes in in-cell NMR experiments.
Related Articles Methyl groups as probes for proteins and complexes in in-cell NMR experiments.
J Am Chem Soc. 2004 Jun 9;126(22):7119-25
Authors: Serber Z, Straub W, Corsini L, Nomura AM, Shimba N, Craik CS, Ortiz de Montellano P, Dötsch V
Studying protein components of large intracellular complexes by in-cell NMR has so far been impossible because the backbone resonances are unobservable due to their slow tumbling rates. We describe a methodology that overcomes...
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11-24-2010 09:51 PM
[NMR paper] 13C-NMR investigation of protein synthesis during apoptosis in human leukemic cell li
13C-NMR investigation of protein synthesis during apoptosis in human leukemic cell lines.
Related Articles 13C-NMR investigation of protein synthesis during apoptosis in human leukemic cell lines.
J Cell Physiol. 1999 Oct;181(1):147-52
Authors: Scott CE, Adebodun F
In order to evaluate the role of protein synthesis in apoptosis, (13)C-NMR has been used to study the levels of protein synthesis in three different human leukemic cell lines in the presence and absence of dexamethasone-induced apoptosis. Measurements were done on one...
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11-18-2010 08:31 PM
[NMR paper] Direct observation of cell wall structure in living plant tissues by solid-state C NM
Direct observation of cell wall structure in living plant tissues by solid-state C NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Direct observation of cell wall structure in living plant tissues by solid-state C NMR spectroscopy.
Plant Physiol. 1990 Jan;92(1):61-5
Authors: Jarvis MC, Apperley DC
Solid-state (13)C nuclear magnetic resonance (NMR) spectra of the following intact plant tissues were recorded by the crosspolarization...
Investigation of proteins in living bacteria with in-cell NMR experiments.
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