Complexins are a family of small presynaptic proteins that regulate neurotransmitter release at nerve terminals and are highly conserved in evolution. While direct interactions with SNARE proteins are critical for all complexin functions, binding of their disordered C-terminal domains (CTD) to membranes, especially to synaptic vesicle membranes, is essential for the ability of complexin to inhibit vesicle release. Furthermore, while some complexin CTDs possess an endogenous affinity for...
[NMR paper] An NMR Approach for Investigating Membrane Protein-Lipid Interactions Using Native Reverse Micelles
An NMR Approach for Investigating Membrane Protein-Lipid Interactions Using Native Reverse Micelles
Peripheral membrane proteins (PMPs) are a subgroup of membrane-associated proteins that are water-soluble and bind to membranes, often reversibly, to perform their function. These proteins have been extensively studied in the aqueous state, but there is often a lack of high-resolution structural and functional studies of these proteins in the membrane-bound state. Currently, nuclear magnetic resonance (NMR) is among the best-equipped methods to study these relatively small proteins and...
Solution NMR of SNAREs, complexin and ?-synuclein in association with membrane-mimetics
Solution NMR of SNAREs, complexin and ?-synuclein in association with membrane-mimetics
Publication date: April 2018
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 105</br>
Author(s): Binyong Liang, Lukas K. Tamm</br>
SNARE-mediated membrane fusion is a ubiquitous process responsible for intracellular vesicle trafficking, including membrane fusion in exocytosis that leads to hormone and neurotransmitter release. The proteins that facilitate this process are highly dynamic and adopt multiple conformations when they interact with other...
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[NMR paper] Solution NMR of SNAREs, complexin and ?-synuclein in association with membrane-mimetics.
Solution NMR of SNAREs, complexin and ?-synuclein in association with membrane-mimetics.
Related Articles Solution NMR of SNAREs, complexin and ?-synuclein in association with membrane-mimetics.
Prog Nucl Magn Reson Spectrosc. 2018 Apr;105:41-53
Authors: Liang B, Tamm LK
Abstract
SNARE-mediated membrane fusion is a ubiquitous process responsible for intracellular vesicle trafficking, including membrane fusion in exocytosis that leads to hormone and neurotransmitter release. The proteins that facilitate this process are highly...
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03-20-2018 10:11 PM
[NMR paper] Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Chemphyschem. 2018 Jan 04;:
Authors: Becker W, Bhattiprolu KC, Gubensäk N, Zangger K
Abstract
Protein-ligand interactions are of fundamental importance in almost all processes in living organisms. The ligands comprise small molecules, drugs or biological macromolecules and their interaction strength varies over several orders of magnitude. Solution NMR spectroscopy offers a large...
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01-10-2018 12:45 PM
[NMR paper] NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
Biochem Biophys Res Commun. 2015 Dec 1;
Authors: Wu J, Domellöf M, Zivkovic AM, Larsson G, Öhman A, Nording ML
Abstract
Low-molecular-weight metabolites in human milk are gaining increasing interest in studies of infant nutrition. In the present study, the milk metabolome...
Investigating Complexin-Membrane Interactions Using NMR and Optical Methods
Linear Mode
