Internal pH indicators for biomolecular NMR

Internal pH indicators for biomolecular NMR

Olga K. Baryshnikova, Thomas C. Williams and Brian D. Sykes
Journal of Biomolecular NMR; 2008; 41(1); pp 5-7

Abstract:
We describe a non-invasive technique for determining pH in biomolecular NMR sample using buffer components (formate, tris, piperazine, and imidazole) as internal pH indicators, whose 1H NMR chemical shifts are sensitive to pH in a range from 2.5 to 9.8. This method is suitable for a wide range of applications where samples are handled intensively during NMR titrations or in high throughput analysis in structural genomics or metabolomics.

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