NMR paper Intergrowth and interfacial structure of biomimetic fluorapatite-gelatin nanocomposite: a solid-state NMR study.

		BioNMR > Educational resources > Journal club
[NMR paper] Intergrowth and interfacial structure of biomimetic fluorapatite-gelatin nanocomposite: a solid-state NMR study.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-05-2014, 03:03 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Intergrowth and interfacial structure of biomimetic fluorapatite-gelatin nanocomposite: a solid-state NMR study.

Related Articles Intergrowth and interfacial structure of biomimetic fluorapatite-gelatin nanocomposite: a solid-state NMR study.

J Phys Chem B. 2014 Jan 23;118(3):724-30

Authors: Vyalikh A, Simon P, Rosseeva E, Buder J, Kniep R, Scheler U

Abstract
The model system fluorapatite-gelatin allows mimicking the formation conditions on a lower level of complexity compared to natural dental and bone tissues. Here, we report on solid-state NMR investigations to examine the structure of fluorapatite-gelatin nanocomposites on a molecular level with particular focus on organic-inorganic interactions. Using (31)P, (19)F, and (1)H MAS NMR and heteronuclear correlations, we found the nanocomposite to consist of crystalline apatite-like regions (fluorapatite and hydroxyfluorapatite) in close contact with a more dissolved (amorphous) layer containing first motifs of the apatite crystal structure as well as the organic component. A scheme of the intergrowth region in the fluorapatite-gelatin nanocomposite, where mineral domains interact with organic matrix, is presented.

PMID: 24354406 [PubMed - indexed for MEDLINE]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Solid-state NMR approaches to study protein structure and protein-lipid interactions. Solid-state NMR approaches to study protein structure and protein-lipid interactions. Solid-state NMR approaches to study protein structure and protein-lipid interactions. Methods Mol Biol. 2013;974:357-87 Authors: Aisenbrey C, Michalek M, Salnikov ES, Bechinger B Abstract Solid-state NMR spectroscopy has been developed for the investigation of membrane-associated polypeptides and remains one of the few techniques to reveal high-resolution structural information in liquid-disordered phospholipid bilayers. In particular, oriented samples...	nmrlearner	Journal club	0	02-14-2013 02:37 PM
(13) C Solid state NMR study of the (13) C-labeled peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG as a model for the local structure of Nephila clavipes dragline silk (MaSp1) before and after spinning. (13) C Solid state NMR study of the (13) C-labeled peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG as a model for the local structure of Nephila clavipes dragline silk (MaSp1) before and after spinning. (13) C Solid state NMR study of the (13) C-labeled peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG as a model for the local structure of Nephila clavipes dragline silk (MaSp1) before and after spinning. Biopolymers. 2011 Sep 12; Authors: Yazawa K, Yamaguchi E, Knight D, Asakura T Abstract We prepared the water soluble model peptide, (E)(8)...	nmrlearner	Journal club	0	09-14-2011 08:07 PM
Heterogeneities in gelatin film formation using single-sided NMR. Heterogeneities in gelatin film formation using single-sided NMR. Heterogeneities in gelatin film formation using single-sided NMR. J Phys Chem B. 2010 Dec 16;114(49):16356-63 Authors: Ghoshal S, Mattea C, Denner P, Stapf S Gelatin solutions were prepared in D(2)O. The drying process of cast solutions was followed with a single-sided nuclear magnetic resonance (NMR) scanner until complete solidification occurred. Spin-spin relaxation times (T(2)) were measured at different layers with microscopic resolution and were correlated with the drying...	nmrlearner	Journal club	0	04-03-2011 06:41 AM
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR. Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR. Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR. J Am Chem Soc. 2011 Jan 5; Authors: Li S, Hong M Histidine structure and chemistry lie at the heart of many enzyme active sites, ion channels, and metalloproteins. While solid-state NMR spectroscopy has been used to study histidine chemical shifts, the full pH dependence of the...	nmrlearner	Journal club	0	01-07-2011 11:21 PM
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR Shenhui Li and Mei Hong http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja108943n/aop/images/medium/ja-2010-08943n_0010.gif Journal of the American Chemical Society DOI: 10.1021/ja108943n http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/FuFM0C9qHyE	nmrlearner	Journal club	0	01-05-2011 11:40 PM
[NMR paper] A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing ch A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine. Related Articles A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine. Chem Phys Lipids. 2004 Nov;132(1):55-64 Authors: Minto RE, Adhikari PR, Lorigan GA Deuterium solid-state NMR spectroscopy was used to qualitatively study the effects of both 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphatidylcholine (PLiPC) and cholesterol on...	nmrlearner	Journal club	0	11-24-2010 10:03 PM
[NMR paper] Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study. Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study. Related Articles Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study. J Biomol Struct Dyn. 1996 Oct;14(2):211-24 Authors: Krushelnitsky AG, Fedotov VD, Spevacek J, Straka J Temperature dependencies of 1H non-selective NMR T1 and T2 relaxation times measured at two resonance frequencies and natural abundance 13C NMR relaxation times T1 and T1r measured at room temperature have been studied in a set of dry and wet solid proteins - Bacterial...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Supercontracted spider dragline silk: a solid-state NMR study of the local structure. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Int J Biol Macromol. 1999 Mar-Apr;24(2-3):173-8 Authors: van Beek JD, Kümmerlen J, Vollrath F, Meier BH The local structure of supercontracted dragline silk from the spider Nephila madagascariensis was investigated by solid-state nuclear magnetic...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off