NMR paper Interaction of synthetic Alzheimer beta-protein-derived analogs with aqueous aluminum

		BioNMR > Educational resources > Journal club
[NMR paper] Interaction of synthetic Alzheimer beta-protein-derived analogs with aqueous aluminum

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:50 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Interaction of synthetic Alzheimer beta-protein-derived analogs with aqueous aluminum

Interaction of synthetic Alzheimer beta-protein-derived analogs with aqueous aluminum: a low-field 27Al NMR investigation.

Related Articles Interaction of synthetic Alzheimer beta-protein-derived analogs with aqueous aluminum: a low-field 27Al NMR investigation.

J Protein Chem. 1995 Nov;14(8):633-44

Authors: Vyas SB, Duffy LK

Synthetic peptides corresponding to the soluble Alzheimer beta-protein, i.e., beta 1-40 and beta 6-25, were utilized to investigate the association of aluminum using low-field 27Al nuclear magnetic resonance (NMR) spectroscopy and reversed-phase high-performance liquid chromatography (RP-HPLC). Addition of beta 1-40 or beta 6-25 to aqueous Al3+ gives rise to a 27Al NMR signal corresponding to the association of Al3+ with the peptides; this effect is not easily reversed by EDTA. Based on the relative intensity of the Al(3+)-peptide signal between pH 4 and 6, there are at least 4 Al3+ ions associated with each peptide molecule. Microheterogeneity is observed with RP-HPLC on incubating solutions of Al3+ with beta 1-40 and beta 6-25. The 27Al NMR spectra of chromatographically pure fractions of beta 1-40 and beta 6-25 indicate that the peptide-associated Al3+ is released below pH 3.5. We propose that soluble beta 1-40 provides an anchor for Al3+ to bind, eventually leading to an increased deposition of amyloid in the Alzheimer brain.

PMID: 8747424 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR and infrared spectroscopic study. Related Articles Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR and infrared spectroscopic study. Biophys J. 2002 Oct;83(4):2074-83 Authors: Richard JA, Kelly I, Marion D, Pézolet M, Auger M The interaction of beta-purothionin, a small basic and antimicrobial protein from the endosperm of wheat seeds, with multilamellar vesicles of dimyristoylphosphatidylglycerol (DMPG) was investigated...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] Comparison of protein backbone entropy and beta-sheet stability: NMR-derived dynamics Comparison of protein backbone entropy and beta-sheet stability: NMR-derived dynamics of protein G B1 domain mutants. Related Articles Comparison of protein backbone entropy and beta-sheet stability: NMR-derived dynamics of protein G B1 domain mutants. J Am Chem Soc. 2001 Jan 10;123(1):185-6 Authors: Stone MJ, Gupta S, Snyder N, Regan L	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] 1H NMR studies on the interaction of beta-carboline derivatives with human serum albu 1H NMR studies on the interaction of beta-carboline derivatives with human serum albumin. Related Articles 1H NMR studies on the interaction of beta-carboline derivatives with human serum albumin. J Magn Reson. 1998 Feb;130(2):281-6 Authors: Veglia G, Delfini M, Giudice MR, Gaggelli E, Valensin G 1H NMR studies were performed on two beta-carboline derivatives interacting with human serum albumin. The spin-lattice relaxation rates of the two derivatives, having side chains of different length and polarity, were used to demonstrate a diverse...	nmrlearner	Journal club	0	11-17-2010 11:06 PM
[NMR paper] Conformation of a beta-adrenoceptor-derived signal transducing peptide as inferred by Conformation of a beta-adrenoceptor-derived signal transducing peptide as inferred by circular dichroism and 1H NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Conformation of a beta-adrenoceptor-derived signal transducing peptide as inferred by circular dichroism and 1H NMR spectroscopy. Biochemistry. 1996 May 21;35(20):6399-405 Authors: Jung H, Windhaber R, Palm D, Schnackerz KD The peptide T345-359 representing the fourth intracellular loop of the avian...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] NMR and circular dichroism studies of synthetic peptides derived from the third intra NMR and circular dichroism studies of synthetic peptides derived from the third intracellular loop of the beta-adrenoceptor. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR and circular dichroism studies of synthetic peptides derived from the third intracellular loop of the beta-adrenoceptor. FEBS Lett. 1995 Jan 23;358(2):133-6 Authors: Jung H, Windhaber R, Palm D, Schnackerz KD The C-terminal part of the third intracellular loop of the beta-adrenoceptor is capable...	nmrlearner	Journal club	0	08-22-2010 03:41 AM
[NMR paper] NMR-derived solution conformations of a hybrid synthetic peptide containing multiple NMR-derived solution conformations of a hybrid synthetic peptide containing multiple epitopes of envelope protein gp120 from the RF strain of human immunodeficiency virus. Related Articles NMR-derived solution conformations of a hybrid synthetic peptide containing multiple epitopes of envelope protein gp120 from the RF strain of human immunodeficiency virus. Biochemistry. 1994 Mar 1;33(8):2055-62 Authors: de Lorimier R, Moody MA, Haynes BF, Spicer LD Solution conformations of a 40-residue hybrid peptide containing T-helper epitopes and B-cell...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] NMR-derived solution conformations of a hybrid synthetic peptide containing multiple NMR-derived solution conformations of a hybrid synthetic peptide containing multiple epitopes of envelope protein gp120 from the RF strain of human immunodeficiency virus. Related Articles NMR-derived solution conformations of a hybrid synthetic peptide containing multiple epitopes of envelope protein gp120 from the RF strain of human immunodeficiency virus. Biochemistry. 1994 Mar 1;33(8):2055-62 Authors: de Lorimier R, Moody MA, Haynes BF, Spicer LD Solution conformations of a 40-residue hybrid peptide containing T-helper epitopes and B-cell...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Conformational preferences of synthetic peptides derived from the immunodominant site Conformational preferences of synthetic peptides derived from the immunodominant site of the circumsporozoite protein of Plasmodium falciparum by 1H NMR. Related Articles Conformational preferences of synthetic peptides derived from the immunodominant site of the circumsporozoite protein of Plasmodium falciparum by 1H NMR. Biochemistry. 1990 Aug 28;29(34):7828-37 Authors: Dyson HJ, Satterthwait AC, Lerner RA, Wright PE Proton nuclear magnetic resonance and ultraviolet circular dichroism spectroscopy have been used to probe the conformational...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off