The role of biomolecular condensates in regulating biological function and the importance of dynamic interactions involving intrinsically disordered protein regions (IDRs) in their assembly are increasingly appreciated. While computational and theoretical approaches have provided significant insights into IDR phase behavior, establishing the critical interactions that govern condensation with atomic resolution through experiment is more difficult, given the lack of applicability of standard...
Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase [Biophysics and Computational Biology]
Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase
Tae Hun Kim, Brandon J. Payliss, Michael L. Nosella, Ian T. W. Lee, Yuki Toyama, Julie D. Forman-Kay, Lewis E. Kay...
Date: 2021-06-01
The role of biomolecular condensates in regulating biological function and the importance of dynamic interactions involving intrinsically disordered protein regions (IDRs) in their assembly are increasingly appreciated. While computational and theoretical approaches have provided significant insights into IDR phase behavior, establishing the critical...
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06-01-2021 11:42 PM
Biomolecular phase separation through the lens of sodium-23 NMR
Biomolecular phase separation through the lens of sodium-23 NMR
Abstract
Phase separation is a fundamental physicochemical process underlying the spatial arrangement and coordination of cellular events. Detailed characterization of biomolecular phase separation requires experimental access to the internal environment of dilute and especially condensed phases at high resolution. In this study, we take advantage from the ubiquitous presence of sodium ions in biomolecular samples and present the potentials of 23Na NMR as a proxy to report the internal fluidity of biomolecular condensed...
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01-28-2021 02:26 AM
Biomolecular phase separation through the lens of sodium-23 NMR
Biomolecular phase separation through the lens of sodium-23 NMR
Abstract
Phase separation is a fundamental physicochemical process underlying the spatial arrangement and coordination of cellular events. Detailed characterization of biomolecular phase separation requires experimental access to the internal environment of dilute and especially condensed phases at high resolution. In this study, we take advantage from the ubiquitous presence of sodium ions in biomolecular samples and present the potentials of 23Na NMR as a proxy to report the internal fluidity of biomolecular condensed...
Relationship of Sequence and Phase Separation in ProteinLow-Complexity Regions
Relationship of Sequence and Phase Separation in ProteinLow-Complexity Regions
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00008/20180316/images/medium/bi-2018-00008n_0006.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00008
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/8TxFxS7r55U
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Phosphate–WaterInterplay Tunes Amorphous CalciumCarbonate Metastability: Spontaneous Phase Separation and Crystallizationvs Stabilization Viewed by Solid State NMR
Phosphate–WaterInterplay Tunes Amorphous CalciumCarbonate Metastability: Spontaneous Phase Separation and Crystallizationvs Stabilization Viewed by Solid State NMR
Shifi Kababya, Assaf Gal, Keren Kahil, Steve Weiner, Lia Addadi and Asher Schmidt
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja511869g/20150105/images/medium/ja-2014-11869g_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/ja511869g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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01-06-2015 07:59 PM
[NMR paper] A solid-state NMR study of molecular mobility and phase separation in co-spray-dried
A solid-state NMR study of molecular mobility and phase separation in co-spray-dried protein-sugar particles.
Related Articles A solid-state NMR study of molecular mobility and phase separation in co-spray-dried protein-sugar particles.
Eur J Pharm Sci. 2005 May;25(1):105-12
Authors: Suihko EJ, Forbes RT, Apperley DC
Molecular mobility and physical form of co-spray-dried sugar-lysozyme formulations were evaluated. Co-spray-dried trehalose:lysozyme and sucrose:lysozyme formulations in 1:9, 1:1 and 9:1 ratios (w:w) were stored at 0% RH and 75%...
Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase
Linear Mode
