[NMR paper] An integrative NMR-SAXS approach for structural determination of large RNAs defines the substrate-free state of a trans-cleaving Neurospora Varkud Satellite ribozyme
An integrative NMR-SAXS approach for structural determination of large RNAs defines the substrate-free state of a trans-cleaving Neurospora Varkud Satellite ribozyme
The divide-and-conquer strategy is commonly used for protein structure determination, but its applications to high-resolution structure determination of RNAs have been limited. Here, we introduce an integrative approach based on the divide-and-conquer strategy that was undertaken to determine the solution structure of an RNA model system, the Neurospora VS ribozyme. NMR and SAXS studies were conducted on a minimal trans VS ribozyme as well as several isolated subdomains. A multi-step procedure...
Evaluation of 15 N-detected Hâ??N correlation experiments on increasingly large RNAs
Evaluation of 15 N-detected Hâ??N correlation experiments on increasingly large RNAs
Abstract
Recently, 15N-detected multidimensional NMR experiments have been introduced for the investigation of proteins. Utilization of the slow transverse relaxation of nitrogen nuclei in a 15N-TROSY experiment allowed recording of high quality spectra for high molecular weight proteins, even in the absence of deuteration. Here, we demonstrate the applicability of three 15N-detected Hâ??N correlation experiments (TROSY, BEST-TROSY and HSQC) to RNA. With the newly...
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19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy
19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy
Abstract
In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted chemical shift dispersion in only four different nucleotides spectral resolution remains limited in larger RNAs. Labeling RNAs with the NMR-active nucleus 19F has previously been introduced for small RNAs up to 40...
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12-28-2015 12:26 AM
[NMR paper] (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
(19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
Related Articles (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.
J Biomol NMR. 2015 Dec 24;
Authors: Sochor F, Silvers R, Müller D, Richter C, Fürtig B, Schwalbe H
Abstract
In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted...
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12-28-2015 12:26 AM
[NMR paper] Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
Related Articles Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
J Struct Funct Genomics. 2014 Apr 20;
Authors: Lengyel J, Hnath E, Storms M, Wohlfarth T
Abstract
Cryo-transmission electron microscopy (Cryo-TEM) and particularly single particle analysis is rapidly becoming the premier method for determining the three-dimensional structure of protein complexes, and viruses....
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04-22-2014 03:54 PM
NMR Localization of Divalent Cations at the ActiveSite of the Neurospora VS Ribozyme Provides Insightsinto RNA–Metal-Ion Interactions
NMR Localization of Divalent Cations at the ActiveSite of the Neurospora VS Ribozyme Provides Insightsinto RNA–Metal-Ion Interactions
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi401484a/aop/images/medium/bi-2013-01484a_0009.gif
Biochemistry
DOI: 10.1021/bi401484a
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/dVjbzJeSUWA
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Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase: pertinence for determining magnetic axes in paramagnetic substrate complexes.
Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase: pertinence for determining magnetic axes in paramagnetic substrate complexes.
Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase: pertinence for determining magnetic axes in paramagnetic substrate complexes.
J Inorg Biochem. 2010 Oct;104(10):1063-70
Authors: Du Z, Unno M, Matsui T, Ikeda-Saito M, La Mar GN
Proton 2D NMR was used to confirm in solution a highly conserved portion of the molecular structure upon substrate loss for the...
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02-10-2011 03:51 PM
Solid-state NMR and SAXS studies provide a structural basis for the activation of alp
Solid-state NMR and SAXS studies provide a structural basis for the activation of alphaB-crystallin oligomers.
Related Articles Solid-state NMR and SAXS studies provide a structural basis for the activation of alphaB-crystallin oligomers.
Nat Struct Mol Biol. 2010 Aug 29;
Authors: Jehle S, Rajagopal P, Bardiaux B, Markovic S, Kühne R, Stout JR, Higman VA, Klevit RE, van Rossum BJ, Oschkinat H
The small heat shock protein alphaB-crystallin (alphaB) contributes to cellular protection against stress. For decades, high-resolution structural studies on...
An integrative NMR-SAXS approach for structural determination of large RNAs defines the substrate-free state of a trans-cleaving Neurospora Varkud Satellite ribozyme
Linear Mode
