NMR spectroscopy is a powerful technique for determining structural and functional features of biomolecules in physiological solution as well as for observing their intermolecular interactions in real-time. However, complex steps associated with its practice have made the approach daunting for non-specialists. We introduce an NMR platform that makes biomolecular NMR spectroscopy much more accessible by integrating tools, databases, web services, and video tutorials that can be launched by simple installation of NMRFAM software packages or using a cross-platform virtual machine that can be run on any standard laptop or desktop computer. The software package can be downloaded freely from the NMRFAM software download page (http://pine.nmrfam.wisc.edu/download_packages.html), and detailed instructions are available from the Integrative NMR Video Tutorial page (http://pine.nmrfam.wisc.edu/integrative.html).
An integrative approach combining ion mobility mass spectrometry, X-ray crystallography and NMR spectroscopy to study the conformational dynamics of ?1-antitrypsin upon ligand binding
An integrative approach combining ion mobility mass spectrometry, X-ray crystallography and NMR spectroscopy to study the conformational dynamics of ?1-antitrypsin upon ligand binding
Abstract
Native mass spectrometry (MS) methods permit the study of multiple protein species within solution equilibria, whilst ion mobility (IM)-MS can report on conformational behaviour of specific states. We used IM-MS to study a conformationally labile protein (?1-antitrypsin) that undergoes pathological polymerisation in the context of point mutations. The folded, native state of the Z variant remains...
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05-26-2015 08:09 PM
[NMR paper] Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
Related Articles Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
J Struct Funct Genomics. 2014 Apr 20;
Authors: Lengyel J, Hnath E, Storms M, Wohlfarth T
Abstract
Cryo-transmission electron microscopy (Cryo-TEM) and particularly single particle analysis is rapidly becoming the premier method for determining the three-dimensional structure of protein complexes, and viruses....
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04-22-2014 03:54 PM
[Nature network NMR forum] Gordon Research Conference: Computational Aspects of Biomolecular NMR, Italy, April 2
Gordon Research Conference: Computational Aspects of Biomolecular NMR, Italy, April 2008 (0 replies)
Next April the biannual Gordon Conference on Biomolecular NMR will be held at the well-known Il Ciocco in Italy. Organized by Rafael Bruschweiler and Stephan Grzesiek this conference promises to present the latest updates in the methodolgical and computational applications of liquid and solid-state NMR.
This is one of the main meetings on the NMR circuit and will be a good opportunity for younger scientists to present their data and network.
Here is the website
Integrative NMR for biomolecular research
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