Related ArticlesIntegration of XAS and NMR techniques for the structure determination of metalloproteins. Examples from the study of copper transport proteins.
J Synchrotron Radiat. 2005 Jan;12(Pt 1):94-7
Authors: Banci L, Bertini I, Mangani S
Nuclear magnetic resonance (NMR) is a powerful technique for protein structure determination in solution. However, when dealing with metalloproteins, NMR methods are unable to directly determine the structure of the metal site and its coordination geometry. The capability of X-ray absorption spectroscopy (XAS) to provide the structure of a metal ion bound to a protein is then perfectly suited to complement the process of the structure determination. This aspect is particularly relevant in structural genomic projects where high throughput of structural results is the main goal. The synergism of the two techniques has been exploited in the structure determination of bacterial copper transport proteins.
[NMRpipe Yahoo group] Integration of Peaks using NMR Draw
Integration of Peaks using NMR Draw
Hello, Using scripts I have created my .ft2 file to visualize my 1D 13C data in nmrDraw. I would like to integrate the area for the carbonyl's and the spinning
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12-21-2011 04:59 PM
2. NMR spectroscopy - Integration
2. NMR spectroscopy - Integration
http://i.ytimg.com/vi/ZrVv7VaRjYs/default.jpg
2. NMR spectroscopy - Integration
Visit www.chemistry.jamesmungall.co.uk for notes on this topic. Thanks for watching! This video explains how integration tells you the number of hydrogen atoms each peak corresponds to. Discussion of how this can be useful in determining structure. Part of a set of videos giving an introductory course on proton NMR, aimed at around A-level or International Baccalaureate standard. Includes dicussion of integration, chemical shift and coupling.
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07-12-2011 08:26 PM
[NMR paper] Integration of NMR and high-throughput screening.
Integration of NMR and high-throughput screening.
Related Articles Integration of NMR and high-throughput screening.
Comb Chem High Throughput Screen. 2002 Dec;5(8):613-21
Authors: Hajduk PJ, Burns DJ
NMR-based screening has become a powerful method for the identification and analysis of low-molecular weight organic compounds that bind to protein targets and can be utilized in drug discovery programs. In particular, heteronuclear NMR-based screening can yield information about both the affinity and binding location of potential lead compounds....
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11-24-2010 08:58 PM
[NMR paper] Simple techniques for the quantification of protein secondary structure by 1H NMR spe
Simple techniques for the quantification of protein secondary structure by 1H NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Simple techniques for the quantification of protein secondary structure by 1H NMR spectroscopy.
FEBS Lett. 1991 Nov 18;293(1-2):72-80
Authors: Wishart DS, Sykes BD, Richards FM
Previous work by Wishart et al. (in press) and others has shown a strong tendency for protein secondary structure to be manifested in 1H NMR chemical...
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08-21-2010 11:12 PM
[NMR paper] Simple techniques for the quantification of protein secondary structure by 1H NMR spe
Simple techniques for the quantification of protein secondary structure by 1H NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Simple techniques for the quantification of protein secondary structure by 1H NMR spectroscopy.
FEBS Lett. 1991 Nov 18;293(1-2):72-80
Authors: Wishart DS, Sykes BD, Richards FM
Previous work by Wishart et al. (in press) and others has shown a strong tendency for protein secondary structure to be manifested in 1H NMR chemical...
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08-21-2010 11:12 PM
[NMR analysis blog] Basis on qNMR: Integration Rudiments (Part II)
Basis on qNMR: Integration Rudiments (Part II)
My last post was a basic survey on different measurement strategies for peak areas. Manual methods such as counting squares or cutting and weighing, known as ‘boundary methods’ were introduced for historical reasons. These methods were first used by engineers, cartographers, etc, end then quickly adopted by spectroscopists and chromatographers.
In the digital era, most common peak area measurement involves the calculation of the running sum of all points within the peak(s) boundaries or by other quadrature method (e.g. Trapezoid, Simpson,...
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08-21-2010 09:12 PM
[NMR analysis blog] Basis on qNMR: Integration Rudiments (Part I)
Basis on qNMR: Integration Rudiments (Part I)
First a quick recap. In my last post I put forward the idea that integration of NMR peaks is the basis of quantitative analysis. Before going any further, I would like to mention that, alternatively, peak heights can also be used for quantitation, but unless some special pre-processing is employed (see for example P. A. Haysa, R. A. Thompson, Magn. Reson. Chem., 2009, 47, 819 – 824, doi) measurement of peak areas is generally the recommended method for qNMR assays.
In this post I will cover some very basic rudiments of NMR peak areas...
Integration of XAS and NMR techniques for the structure determination of metalloprote
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