Related ArticlesInsights into protein stability in cell lysate by 19F NMR spectroscopy.
Chembiochem. 2020 Aug 12;:
Authors: Welte H, Kovermann M
Abstract
In living organisms, protein folding and function take place in an inhomogeneous, highly crowded environment possessing a concentration of diverse macromolecules of up to 400*g/L. It has been shown that the intracellular environment has a pronounced effect on the stability, dynamics and function of the protein under study and has for this reason to be considered. However, most protein studies are neglecting the presence of these macromolecules. Consequently, we probe here the overall thermodynamic stability of cold shock protein B from* Bacillus subtilis *( Bs CspB) in cell lysate. We found that an increase in cell lysate concentration causes a monotonic increase in thermodynamic stability of* Bs CspB. This result strongly underlines the importance of considering the biological environment when inherent protein parameters shall be quantitatively determined. Moreover, we demonstrate that the targeted application of* 19 F NMR spectroscopy operates as an ideal tool utilized to protein studies performed in complex cellular surroundings.
PMID: 32786103 [PubMed - as supplied by publisher]
[NMR paper] How does the mood stabilizer lithium bind ATP, the energy Earnings of the cell: Insights from solid-state NMR.
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Biochim Biophys Acta Gen Subj. 2020 01;1864(1):129456
Authors: Haimovich A, Goldbourt A
Abstract
BACKGROUND: Lithium, in the form of a salt, is a mood stabilizer and a...
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Programmed cell death: Solid-state NMR insights
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http://www.spectroscopynow.com/common/images/thumbnails/169b46e7c0b.jpgResearchers from The Netherlands and the USA have used solid-state nuclear magnetic resonance (NMR) spectroscopy to show how the signal induced by the enzyme cytochrome C is more controlled than was originally anticipated. The discovery could have implications for understanding mitochondria and programmed cell death.
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[NMR paper] 15N isotopic labelling for in-cell protein studies by NMR spectroscopy and single-cell IR synchrotron radiation FTIR microscopy: a correlative study.
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Analyst. 2018 Feb 06;:
Authors: Mitri E, Barbieri L, Vaccari L, Luchinat E
Abstract
The ultimate goal of modern structural biology is to probe protein structures and dynamics in their physiological microenvironment. In-cell NMR...
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02-07-2018 03:41 PM
[NMR paper] Insights into the Binding of Cyclic RGD Peptidomimetics to ?5?1 Integrin by using Live-Cell NMR And Computational Studies.
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ChemistryOpen. 2017 Feb;6(1):128-136
Authors: Guzzetti I, Civera M, Vasile F, Arosio D, Tringali C, Piarulli U, Gennari C, Pignataro L, Belvisi L, Potenza D
Abstract
The interaction of a small library of cyclic DKP-RGD peptidomimetics with ?5?1 integrin has been...
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[NMR paper] Protein Structure Determination by Magic-Angle Spinning Solid-State NMR, and Insights into the Formation, Structure, and Stability of Amyloid Fibrils.
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Annu Rev Biophys. 2013 Mar 22;
Authors: Comellas G, Rienstra CM
Abstract
Protein structure determination methods using magic-angle spinning solidstate nuclear magnetic resonance (MAS SSNMR) have experienced a remarkable...
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[NMR paper] Permeation through Phospholipid Bilayers, Skin-Cell Penetration, Plasma Stability, and CD Spectra of ?- and ?-Oligoproline Derivatives.
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Chem Biodivers. 2013 Jan;10(1):1-38
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[NMR paper] Probing non-specific interactions of Ca(2+)-calmodulin in E. coli lysate.
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J Biomol NMR. 2013 Jan 17;
Authors: Latham MP, Kay LE
Abstract
The biological environment in which a protein performs its function is a crowded milieu containing millions of molecules that can potentially lead to a great many transient, non-specific...
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[NMR paper] Insights into the determinants of beta-sheet stability: 1H and 13C NMR conformational
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J Pept Res. 2003 Apr;61(4):177-88
Authors: Santiveri CM, Rico M, Jiménez MA, Pastor MT, Pérez-Payá E
In a previous study we designed a 20-residue peptide able to adopt a significant population of a three-stranded...
Insights into protein stability in cell lysate by 19F NMR spectroscopy.
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