BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search
Home Forums Wiki NMR feeds Downloads Register Today's Posts



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
 
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Old 10-19-2017, 10:47 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,777
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Insights into co-translational membrane protein insertion by combined LILBID-mass spectrometry and NMR spectroscopy.

Insights into co-translational membrane protein insertion by combined LILBID-mass spectrometry and NMR spectroscopy.

Related Articles Insights into co-translational membrane protein insertion by combined LILBID-mass spectrometry and NMR spectroscopy.

Anal Chem. 2017 Oct 17;:

Authors: Peetz O, Henrich E, Laguerre A, L?hr F, Hein C, Dötsch V, Bernhard F, Morgner N

Abstract
Co-translational insertion of membrane proteins into defined nanoparticle membranes has been developed as an efficient process to produce highly soluble samples in native-like environments and to study lipid dependent effects on protein structure and function. Numerous examples of structural and functional characterization of transporters, ion channels or G-protein coupled receptors in co-translationally formed nanodisc complexes demonstrate the versatility of this approach, although the basic underlying mechanisms of membrane insertion are mainly unknown. We have revealed first aspects of the insertion of proteins into nanodiscs by combining cell-free expression, non-covalent mass spectrometry and NMR spectroscopy. We provide evidence of cooperative insertion of homooligomeric complexes and demonstrate the possibility to modulate their stoichiometry by modifying reaction conditions. Additionally, we show that significant amounts of lipid are released from the nanodiscs upon insertion of larger protein complexes.


PMID: 29039652 [PubMed - as supplied by publisher]



More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMR paper] Combined Use of MALDI-TOF Mass Spectrometry and (31)P NMR Spectroscopy for Analysis of Phospholipids.
Combined Use of MALDI-TOF Mass Spectrometry and (31)P NMR Spectroscopy for Analysis of Phospholipids. Related Articles Combined Use of MALDI-TOF Mass Spectrometry and (31)P NMR Spectroscopy for Analysis of Phospholipids. Methods Mol Biol. 2017;1609:107-122 Authors: Schröter J, Popkova Y, Süß R, Schiller J Abstract Lipids are important and abundant constituents of all biological tissues and body fluids. In particular, phospholipids (PL) constitute a major part of the cellular membrane, play a role in signal transduction, and some...
nmrlearner Journal club 0 07-01-2017 11:41 AM
[NMR paper] Flexible stoichiometry and asymmetry of the PIDDosome core complex by heteronuclear NMR spectroscopy and mass spectrometry.
Flexible stoichiometry and asymmetry of the PIDDosome core complex by heteronuclear NMR spectroscopy and mass spectrometry. Flexible stoichiometry and asymmetry of the PIDDosome core complex by heteronuclear NMR spectroscopy and mass spectrometry. J Mol Biol. 2014 Dec 17; Authors: Nematollahi LA, Garza-Garcia A, Bechara C, Esposito D, Morgner N, Robinson CV, Driscoll PC Abstract Homotypic death domain (DD)-DD interactions are important in the assembly of oligomeric signalling complexes such as the PIDDosome that acts as a...
nmrlearner Journal club 0 12-22-2014 08:42 PM
Proteomics Market (Protein Microarray, Mass Spectrometry, NMR Spectroscopy ... - MENAFN.COM
Proteomics Market (Protein Microarray, Mass Spectrometry, NMR Spectroscopy ... - MENAFN.COM <img alt="" height="1" width="1" /> Proteomics Market (Protein Microarray, Mass Spectrometry, NMR Spectroscopy ... MENAFN.COM Feb 12, 2014 (Menafn - M2 PRESSWIRE via COMTEX) --The "Proteomics Market (Protein Microarray, Mass Spectrometry, NMR Spectroscopy, Chromatography, Electrophoresis, Surface Plasmon Resonance, Protein Fractionation, X-ray Crystallography, ... Read here
nmrlearner Online News 0 02-12-2014 11:48 AM
[NMR paper] Native mass spectrometry of photosynthetic pigment-protein complexes.
Native mass spectrometry of photosynthetic pigment-protein complexes. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Native mass spectrometry of photosynthetic pigment-protein complexes. FEBS Lett. 2013 Jan 18; Authors: Zhang H, Cui W, Gross ML, Blankenship RE Abstract Native mass spectrometry (MS), or as is sometimes called "native electrospray ionization" allows proteins in their native or near-native states in solution to be introduced into the gas phase and...
nmrlearner Journal club 0 02-03-2013 10:19 AM
Interactions between CusF and CusB Identified by NMR Spectroscopy and Chemical Cross-Linking Coupled to Mass Spectrometry
Interactions between CusF and CusB Identified by NMR Spectroscopy and Chemical Cross-Linking Coupled to Mass Spectrometry http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi102012j/aop/images/medium/bi-2010-02012j_0006.gif Biochemistry DOI: 10.1021/bi102012j http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/1zthtU6QJBQ More...
nmrlearner Journal club 0 03-09-2011 04:19 AM
Interactions between CusF and CusB identified by NMR spectroscopy and chemical cross-linking coupled to mass spectrometry.
Interactions between CusF and CusB identified by NMR spectroscopy and chemical cross-linking coupled to mass spectrometry. Interactions between CusF and CusB identified by NMR spectroscopy and chemical cross-linking coupled to mass spectrometry. Biochemistry. 2011 Feb 16; Authors: Mealman TD, Bagai I, Singh P, Goodlet DR, Rensing C, Zhou H, Wysocki VH, McEvoy MM The E. coli periplasmic proteins CusF and CusB, as part of the CusCFBA efflux system, aid in the resistance of elevated levels of copper and silver by direct metal transfer between the...
nmrlearner Journal club 0 02-18-2011 08:07 PM
[NMR paper] Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spect
Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist. Related Articles Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist. Structure. 1994 Jun 15;2(6):465-7 Authors: Chait BT
nmrlearner Journal club 0 08-22-2010 03:33 AM
[NMR paper] Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spect
Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist. Related Articles Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist. Structure. 1994 Jun 15;2(6):465-7 Authors: Chait BT
nmrlearner Journal club 0 08-22-2010 03:33 AM



Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 03:00 PM.


Map