Related ArticlesThe influence of internuclear spatial distribution and instrument noise on the precision of distances determined by solid state NMR of isotopically enriched proteins.
J Biomol NMR. 2003 Nov;27(3):235-59
Authors: Gehman JD, Paulson EK, Zilm KW
The relative merits of different isotopic enrichment strategies that might be used in solid state NMR protein structure determinations are explored. The basis for comparison of these merits is the determination of the relative uncertainties in rates measured by a generalized dipolar recoupling experiment. The different schemes considered use (13)C, (15)N and (2)H labeling of ubiquitin with homonuclear magnetization-transfer type experiments under magic-angle spinning (MAS). Specific attention is given to the sensitivity of the predicted relative precisions to variation in natural nuclear density distribution and noise levels. A framework is suggested to gauge the precision of measurement of a given dipolar coupling constant, and the potential for a set of such measurements to constrain structure calculations is explored. The distribution of nuclei in homonuclear (15)N and (1)H dipolar recoupling spin-exchange experiments appear to provide the most promising tertiary structure information for uniformly labeled ubiquitin.
[NMRpipe Yahoo group] Re: Signal-to-noise estimation
Re: Signal-to-noise estimation
Greetings, Dear Pipers, and Happy Winter Holidays, or Whatever ... There are lots of ways this could be done, depending on how rigorous you want to be, how
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12-25-2011 10:35 PM
[NMRpipe Yahoo group] Signal-to-noise estimation
Signal-to-noise estimation
Hello! I am new in "NMR world". I have some basic questions regarding the NMRPipe. I would like to know if there is a manner to estimate the signal-to-noise
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[Question from NMRWiki Q&A forum] poor signal to noise on 1H-15N heteronculear NOE
poor signal to noise on 1H-15N heteronculear NOE
I have recently attempted to run a 1H-15N heteronuclear NOE experiment in vnmrj (700 rt probe) using the standard sequence that comes with biopack, the signal to nose im getting compared to my standard HSQC is significantly reduced, is this normal? I do not recall this being the case when I ran this previously on a bruker 600 with RT probe? is this normal or does this suggest something is miss set? Also in the no NOE experiment I get very broad noise in the centre of my spectrum/water line.
regards
Tom
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11-01-2011 01:52 AM
[NMR paper] Analysis of error propagation from NMR-derived internuclear distances into molecular
Analysis of error propagation from NMR-derived internuclear distances into molecular structure of cyclo-pro-gly.
Related Articles Analysis of error propagation from NMR-derived internuclear distances into molecular structure of cyclo-pro-gly.
J Magn Reson. 1998 Dec;135(2):454-65
Authors: Dzakula Z, Jurani? , DeRider ML, Westler WM, Macura S, Markley JL
Analytical expressions have been derived that translate uncertainties in distance constraints (obtained from NMR investigations) into uncertainties in atom positions in the maximum likelihood...
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11-17-2010 11:15 PM
[NMR paper] Solution structure of GAP SH3 domain by 1H NMR and spatial arrangement of essential R
Solution structure of GAP SH3 domain by 1H NMR and spatial arrangement of essential Ras signaling-involved sequence.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Solution structure of GAP SH3 domain by 1H NMR and spatial arrangement of essential Ras signaling-involved sequence.
EMBO J. 1994 Mar 15;13(6):1270-9
Authors: Yang YS, Garbay C, Duchesne M, Cornille F, Jullian N, Fromage N, Tocque B, Roques BP
Src homology 3 (SH3) domains are found in numerous...
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08-22-2010 03:33 AM
[NMR Geek blog] Fluorescence: A source of noise in NMR?
Fluorescence: A source of noise in NMR?
The other day I was reading a book “Experimental Pulse NMR: A Nuts and Bolt Approach” by Eiichi Fukushima and Stephen B W Roeder and I was encountered with a sentence which says,”Fluorescent lights generate noise which can be picked up in the spectrometer.” (para 1, page 13). I wonder if they are talking about
Full story can be found on the NMR geek blog
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08-22-2010 01:41 AM
[Stan NMR blog] Noise Figure and Equivalent Input Noise
Noise Figure and Equivalent Input Noise
An educational article about the definitions of - and the relation between - the two concepts.
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08-21-2010 06:14 PM
De novo determination of internuclear vector orientations from residual dipolar couplings measured in three independent alignment media
De novo determination of internuclear vector orientations from residual dipolar couplings measured in three independent alignment media
Ke Ruan, Kathryn B. Briggman and Joel R. Tolman
Journal of Biomolecular NMR; 2008; 41(2) pp 61 - 76
Abstract:
The straightforward interpretation of solution state residual dipolar couplings (RDCs) in terms of internuclear vector orientations generally requires prior knowledge of the alignment tensor, which in turn is normally estimated using a structural model. We have developed a protocol which allows the requirement for prior structural knowledge to...
The influence of internuclear spatial distribution and instrument noise on the precis
Linear Mode
