Investigation of the Binding Interaction of FattyAcids with Human G Protein-Coupled Receptor 40 Using a Site-SpecificFluorescence Probe by Flow Cytometry
Investigation of the Binding Interaction of FattyAcids with Human G Protein-Coupled Receptor 40 Using a Site-SpecificFluorescence Probe by Flow Cytometry
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00079/20160317/images/medium/bi-2016-00079r_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00079
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nmrlearner
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[NMR paper] Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
Nat Commun. 2015;6:8202
Authors: Yang F, Yu X, Liu C, Qu CX, Gong Z, Liu HD, Li FH, Wang HM, He DF, Yi F, Song C, Tian CL, Xiao KH, Wang JY, Sun JP
Abstract
Specific arrestin conformations are coupled to distinct downstream effectors, which underlie the functions of many...
nmrlearner
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09-09-2015 11:49 AM
[NMR paper] Influence of the incorporation of fibers in biscuit dough on proton mobility characterized by time domain NMR.
Influence of the incorporation of fibers in biscuit dough on proton mobility characterized by time domain NMR.
Influence of the incorporation of fibers in biscuit dough on proton mobility characterized by time domain NMR.
Food Chem. 2016 Feb 1;192:950-7
Authors: Serial MR, Blanco Canalis MS, Carpinella M, Valentinuzzi MC, León AE, Ribotta PD, Acosta RH
Abstract
The effect of fiber addition on the distribution and mobility of protons in biscuits is studied by using low resolution time domain nuclear magnetic resonance (TD-NMR)....
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Paramagnetic relaxation enhancement of membrane proteins by incorporation of the metal-chelating unnatural amino acid 2-amino-3-(8-hydroxyquinolin-3-yl)propanoic acid (HQA)
Paramagnetic relaxation enhancement of membrane proteins by incorporation of the metal-chelating unnatural amino acid 2-amino-3-(8-hydroxyquinolin-3-yl)propanoic acid (HQA)
Abstract
The use of paramagnetic constraints in protein NMR is an active area of research because of the benefits of long-range distance measurements (>10Â*Ã?). One of the main issues in successful execution is the incorporation of a paramagnetic metal ion into diamagnetic proteins. The most common metal ion tags are relatively long aliphatic chains attached to the side chain of a...
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11-28-2014 11:37 AM
[NMR paper] NMR conformational dynamics of an Anthrax Lethal Factor domain studied by multiple amino acid-selective labeling.
NMR conformational dynamics of an Anthrax Lethal Factor domain studied by multiple amino acid-selective labeling.
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Biochem Biophys Res Commun. 2014 Jun 2;
Authors: Vourtsis DJ, Chasapis CT, Pairas G, Bentrop D, Spyroulias GA
Abstract
NMR-based structural biology urgently needs cost- and time-effective methods to assist both in the process of acquiring high-resolution NMR spectra and their subsequent...
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An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media
An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media
Abstract The in vivo incorporation of unnatural amino acids into proteins is a well-established technique requiring an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is incorporated at a position encoded by a TAG amber codon. Although this technology provides unique opportunities to engineer protein structures, poor protein yields are usually obtained in deuterated media, hampering its application in the protein NMR...
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02-21-2012 03:40 AM
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Comp Biochem Physiol A Mol Integr Physiol. 2011 Jul;159(3):219-24
Authors: Creager MS, Izdebski T, Brooks AE, Lewis RV
Abstract
Spider silk has been evolutionarily optimized for contextual mechanical performance over the last 400 Ma. Despite precisely balanced mechanical properties,...
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09-02-2011 05:40 PM
[NMR paper] An AMBER/DYANA/MOLMOL phosphorylated amino acid library set and incorporation into NMR structure calculations.
An AMBER/DYANA/MOLMOL phosphorylated amino acid library set and incorporation into NMR structure calculations.
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J Biomol NMR. 2005 Sep;33(1):15-24
Authors: Craft JW, Legge GB
Protein structure determination using Nuclear Magnetic Resonance (NMR) requires the use of molecular dynamics programs that incorporate both NMR experimental and implicit atomic data. Atomic parameters for each amino acid type are encoded in...
Incorporation of an Unnatural Amino Acid as a Domain-SpecificFluorescence Probe in a Two-Domain Protein
Linear Mode
