Apodization weighted acquisition is a simple approach to enhance the sensitivity of multidimensional NMR spectra by scaling the number of scans during acquisition of the indirect dimension(s). The signal content of the resulting spectra is identical to conventionally sampled data, yet the spectra show improved signal-to-noise ratios. There are no special requirements for data acquisition and processing: the time-domain data can be transformed with the same schemes used for conventionally recorded spectra, including Fourier transformation. The method is of general use in multidimensional liquid and solid state NMR experiments if the number of recorded transients per sampling point is bigger than the minimum required phase cycle of the pulse sequence.
[NMR paper] Nonuniform Sampling in Multidimensional NMR for Improving Spectral Sensitivity.
Nonuniform Sampling in Multidimensional NMR for Improving Spectral Sensitivity.
Nonuniform Sampling in Multidimensional NMR for Improving Spectral Sensitivity.
Methods. 2018 Mar 06;:
Authors: Zambrello MA, Schuyler AD, Maciejewski MW, Delaglio F, Bezsonova I, Hoch JC
Abstract
The development of multidimensional NMR spectroscopy enabled an explosion of structural and dynamical investigations on proteins and other biomacromolecules. Practical limitations on data sampling, based on the Jeener paradigm of parametric sampling of...
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03-10-2018 04:36 PM
Sensitivity of nonuniform sampling NMR
From The DNP-NMR Blog:
Sensitivity of nonuniform sampling NMR
This is not an article directly related to DNP, however, non-uniform sampling is another great technique to enhance sensitivity in a NMR experiment. The concept can easily combined with DNP to yield even higher sensitivity enhancement factors.
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07-17-2015 03:02 PM
[NMR paper] Performance tuning non-uniform sampling for sensitivity enhancement of signal-limited biological NMR.
Performance tuning non-uniform sampling for sensitivity enhancement of signal-limited biological NMR.
Related Articles Performance tuning non-uniform sampling for sensitivity enhancement of signal-limited biological NMR.
J Biomol NMR. 2014 Mar 29;
Authors: Palmer MR, Wenrich BR, Stahlfeld P, Rovnyak D
Abstract
Non-uniform sampling (NUS) has been established as a route to obtaining true sensitivity enhancements when recording indirect dimensions of decaying signals in the same total experimental time as traditional uniform...
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04-01-2014 02:47 PM
An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR
An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR
June 2012
Publication year: 2012
Source:Journal of Magnetic Resonance, Volume 219</br>
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Non-uniform weighted sampling (NUWS) is a sampling strategy, related to non-uniform sampling (NUS) in the limit of long acquisition times, in which each indirect increment of a multidimensional spectrum is sampled multiple times according to some weighting function. As the spectrum is fully sampled it can be processed in a conventional manner by the...
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02-03-2013 10:13 AM
An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR
An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Christopher A. Waudby, John Christodoulou</br>
Non-uniform weighted sampling (NUWS) is a sampling strategy, related to non-uniform sampling (NUS) in the limit of long acquisition times, in which each indirect increment of a multidimensional spectrum is sampled multiple times according to some weighting function. As the spectrum is fully sampled it can be processed in a conventional...
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05-01-2012 08:03 PM
Grid computing for improving conformational sampling in NMR structure calculation.
Grid computing for improving conformational sampling in NMR structure calculation.
Grid computing for improving conformational sampling in NMR structure calculation.
Bioinformatics. 2011 May 5;
Authors: Mareuil F, Blanchet C, Malliavin TE, Nilges M
MOTIVATION: Methods for automatic NMR structure determination need to face a high level of ambiguity encountered in NMR spectra recorded by solid-state NMR and by solution NMR of partially unfolded proteins, leading to time-consuming calculations. The software package Ambiguous Restraints for Iterative...
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05-08-2011 04:35 AM
[NMR paper] Improving NMR sensitivity in room temperature and cooled probes with dipolar ions.
Improving NMR sensitivity in room temperature and cooled probes with dipolar ions.
Related Articles Improving NMR sensitivity in room temperature and cooled probes with dipolar ions.
J Magn Reson. 2005 Apr;173(2):339-43
Authors: Lane AN, Arumugam S
The response of inverse triple resonance cold and conventional probes to ionic strength has been compared under a variety of conditions relevant to protein NMR. Increasing the salt concentration degrades probe performance in terms of sensitivity, and the effect is more severe for cold probes and...
Improving the sensitivity of FT-NMR spectroscopy by apodization weighted sampling
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