NMR metabolomics has inherent capabilities for studying biofluids, such as reproducibility, minimal sample preparation, non-destructiveness, and molecular structure elucidation; however, reliable quantitation of metabolites is still a challenge because of the complex matrix of the samples. The serum is one of the most common samples in clinical studies but possibly the most difficult for NMR analysis because of the high content of proteins, which hampers the detection and quantification of...
[NMR paper] Beyond the limit of assignment of metabolites using minimal serum samples and 1H NMR spectroscopy with cross-validation by mass spectrometry.
Beyond the limit of assignment of metabolites using minimal serum samples and 1H NMR spectroscopy with cross-validation by mass spectrometry.
Beyond the limit of assignment of metabolites using minimal serum samples and 1H NMR spectroscopy with cross-validation by mass spectrometry.
J Pharm Biomed Anal. 2018 Jan 09;151:356-364
Authors: Gupta A, Kumar D
Abstract
Identification of NMR-based metabolic indexes is limited by the deleterious effects of copious proteins and lipoproteins in the serum that accentuate the need for advance...
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02-08-2018 04:32 PM
[NMR paper] NMR spectroscopy of filtered serum of prostate cancer: A new frontier in metabolomics.
NMR spectroscopy of filtered serum of prostate cancer: A new frontier in metabolomics.
Related Articles NMR spectroscopy of filtered serum of prostate cancer: A new frontier in metabolomics.
Prostate. 2016 Sep;76(12):1106-19
Authors: Kumar D, Gupta A, Mandhani A, Sankhwar SN
Abstract
BACKGROUND: To address the shortcomings of digital rectal examinations (DRE), serum prostate-specific antigen (PSA), and trans-rectal ultrasound (TRUS) for precise determination of prostate cancer (PC) and differentiation from benign prostatic...
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07-14-2017 01:53 PM
Journal Highlight: NMR spectroscopy of filtered serum of prostate cancer: A new frontier in metabolomics
Journal Highlight: NMR spectroscopy of filtered serum of prostate cancer: A new frontier in metabolomics
http://www.spectroscopynow.com/common/images/thumbnails/1563b836aed.jpgThe application of 1H NMR spectroscopy of 52 serum metabolites as a surrogate tactic for probing and predicting prostate cancer and benign prostatic hyperplasia is on a par with LC/MS and histopathological examination.
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08-01-2016 10:03 AM
[NMR paper] (1)H NMR-Derived Metabolomics of Filtered Serum of Myocardial Ischemia in Unstable Angina Patients.
(1)H NMR-Derived Metabolomics of Filtered Serum of Myocardial Ischemia in Unstable Angina Patients.
(1)H NMR-Derived Metabolomics of Filtered Serum of Myocardial Ischemia in Unstable Angina Patients.
Clin Chim Acta. 2016 Feb 26;
Authors: Ameta K, Gupta A, Ameta D, Sethi R, Kumar D, Ahmad I, Mahdi AA
Abstract
BACKGROUND: Despite continuing research for development of accurate biomarkers of myocardial ischemia in unstable angina, lack of biochemical biomarkers is alarming. We sought to develop accurate biomarkers using high...
Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks
Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks
Abstract A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freezeâ??thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of...
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10-05-2011 08:57 PM
The application of micro-coil NMR probe technology to metabolomics of urine and serum
The application of micro-coil NMR probe technology to metabolomics of urine and serum
Abstract Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5 mm NMR probe. Sample concentration is evaluated as a means to leverage the...
An improved NMR approach for metabolomics of intact serum samples
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