NMR spectroscopy is the only method to access the structural dynamics of biomolecules at high (atomistic) resolution in their native solution state. However, this method's low sensitivity has two important consequences: (i) typically experiments have to be performed at high concentrations that increase sensitivity but are not physiological, and (ii) signals have to be accumulated over long periods, complicating the determination of interaction kinetics on the order of seconds and impeding...
Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water
Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water
Abstract
Signal enhancements of up to two orders of magnitude in protein NMR can be achieved by employing HDO as a vector to introduce hyperpolarization into folded or intrinsically disordered proteins. In this approach, hyperpolarized HDO produced by dissolution-dynamic nuclear polarization (D-DNP) is mixed with a protein solution waiting in a high-field NMR spectrometer, whereupon amide proton exchange and nuclear Overhauser effects...
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Deuteration of Hyperpolarized 13 C-Labeled Zymonic Acid Enables Sensitivity-Enhanced Dynamic MRI of pH
From The DNP-NMR Blog:
Deuteration of Hyperpolarized 13 C-Labeled Zymonic Acid Enables Sensitivity-Enhanced Dynamic MRI of pH
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Hundshammer, C., et al., Deuteration of Hyperpolarized 13 C-Labeled Zymonic Acid Enables Sensitivity-Enhanced Dynamic MRI of pH. ChemPhysChem, 2017. 18(18): p. 2422-2425.
https://www.ncbi.nlm.nih.gov/pubmed/28719100
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10-09-2017 04:12 PM
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
From The DNP-NMR Blog:
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
Olsen, G., et al., Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR. J Magn Reson, 2016. 264: p. 49-58.
http://www.ncbi.nlm.nih.gov/pubmed/26920830
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05-09-2016 04:04 PM
[NMR paper] Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.
Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Aug 3;54(32):9162-85
Authors: Ardenkjaer-Larsen JH, Boebinger GS, Comment A, Duckett S, Edison AS, Engelke F, Griesinger C, Griffin RG, Hilty C, Maeda H, Parigi G, Prisner T, Ravera E, van Bentum J, Vega S, Webb A,...
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05-07-2016 06:32 PM
[NMR paper] Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR.
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR.
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR.
J Magn Reson. 2016 Mar;264:49-58
Authors: Olsen G, Markhasin E, Szekely O, Bretschneider C, Frydman L
Abstract
A recent study explored the use of hyperpolarized water, to enhance the sensitivity of nuclei in biomolecules thanks to rapid proton exchanges with labile amide backbone and sidechain groups. Further optimizations of this...
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02-28-2016 03:10 PM
[NMR paper] Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
Publication date: March 2016
Source:Journal of Magnetic Resonance, Volume 264</br>
Author(s): Greg Olsen, Evgeny Markhasin, Or Szekely, Christian Bretschneider, Lucio Frydman</br>
A recent study explored the use of hyperpolarized water, to enhance the sensitivity of nuclei in biomolecules thanks to rapid proton exchanges with labile amide backbone and sidechain groups. Further optimizations of this approach have now allowed us to achieve proton polarizations...
On the Potential of Hyperpolarized Water in Biomolecular NMR Studies
From The DNP-NMR Blog:
On the Potential of Hyperpolarized Water in Biomolecular NMR Studies
Harris, T., O. Szekely, and L. Frydman, On the Potential of Hyperpolarized Water in Biomolecular NMR Studies. The Journal of Physical Chemistry B, 2014. 118(12): p. 3281-3290.
http://dx.doi.org/10.1021/jp4102916
Hyperpolarized water as universal sensitivity booster in biomolecular NMR
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