NMR paper Hydrogen exchange properties of proteins in native and denatured states monitored by

		BioNMR > Educational resources > Journal club
[NMR paper] Hydrogen exchange properties of proteins in native and denatured states monitored by

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:31 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Hydrogen exchange properties of proteins in native and denatured states monitored by

Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR.

Related Articles Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR.

Protein Sci. 1997 Jun;6(6):1316-24

Authors: Chung EW, Nettleton EJ, Morgan CJ, Gross M, Miranker A, Radford SE, Dobson CM, Robinson CV

The extent of deuterium labeling of hen lysozyme, its three-disulfide derivative, and the homologous alpha-lactalbumins, has been measured by both mass spectrometry and NMR. Different conformational states of the proteins were produced by varying the solution conditions. Alternate protein conformers were found to contain different numbers of 2H atoms. Furthermore, measurement in the gas phase of the mass spectrometer or directly in solution by NMR gave consistent results. The unique ability of mass spectrometry to distinguish distributions of 2H atoms in protein molecules is exemplified using samples prepared to contain different populations of 2H-labeled protein. A comparison of the peak widths of bovine alpha-lactalbumin in alternate solution conformations but containing the same average number of 2H atoms showed dramatic differences due to different 2H distributions in the two protein conformers. Measurement of 2H distributions by ESI-MS enabled characterization of conformational averaging and structural heterogeneity. In addition, a time course for hydrogen exchange was examined and the variation in distributions of 2H atom compared with simulations for different hydrogen exchange models. The results clearly show that exchange from the native state of bovine alpha-lactalbumin at 15 degrees C is dominated by local unfolding events.

PMID: 9194192 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Ribonuclease Sa conformational stability studied by NMR-monitored hydrogen exchange. Ribonuclease Sa conformational stability studied by NMR-monitored hydrogen exchange. Related Articles Ribonuclease Sa conformational stability studied by NMR-monitored hydrogen exchange. Biochemistry. 2005 May 31;44(21):7644-55 Authors: Laurents DV, Scholtz JM, Rico M, Pace CN, Bruix M The conformational stability of ribonuclease Sa (RNase Sa) has been measured at the per-residue level by NMR-monitored hydrogen exchange at pH* 5.5 and 30 degrees C. In these conditions, the exchange mechanism was found to be EXII. The conformational stability...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] Hydrodynamic radii of native and denatured proteins measured by pulse field gradient Hydrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques. Related Articles Hydrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques. Biochemistry. 1999 Dec 14;38(50):16424-31 Authors: Wilkins DK, Grimshaw SB, Receveur V, Dobson CM, Jones JA, Smith LJ Pulse field gradient NMR methods have been used to determine the effective hydrodynamic radii of a range of native and nonnative protein conformations. From these experimental data, empirical relationships...	nmrlearner	Journal club	0	11-18-2010 08:31 PM
[NMR paper] Pressure-denatured state of Escherichia coli ribonuclease HI as monitored by Fourier Pressure-denatured state of Escherichia coli ribonuclease HI as monitored by Fourier transform infrared and NMR spectroscopy. Related Articles Pressure-denatured state of Escherichia coli ribonuclease HI as monitored by Fourier transform infrared and NMR spectroscopy. Biochemistry. 1998 Dec 22;37(51):18001-9 Authors: Yamasaki K, Taniguchi Y, Takeda N, Nakano K, Yamasaki T, Kanaya S, Oobatake M Pressure denaturation of Escherichia coli ribonuclease HI (RNase HI) was studied by Fourier transform infrared (FTIR) and two-dimensional NMR...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Hydrogen exchange properties of proteins in native and denatured states monitored by Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR. Protein Sci. 1997 Jun;6(6):1316-24 Authors: Chung EW,...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] Structural analysis of non-native states of proteins by NMR methods. Structural analysis of non-native states of proteins by NMR methods. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural analysis of non-native states of proteins by NMR methods. Curr Opin Struct Biol. 1996 Feb;6(1):24-30 Authors: Shortle DR Established NMR methods are increasingly being applied to the non-native states of proteins. For small denatured proteins, full assignment of proton, 15N and 13C resonances is often straightforward. Sensitive methods exist...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[MWClarkson blog] How native-like is a cold-denatured structure? How native-like is a cold-denatured structure? http://www.researchblogging.org/public/citation_icons/rb2_large_gray.pngA protein has several different levels of structure. The primary structure is the arrangements of atoms and bonds, and it is formed in the ribosome by the assembly of amino acids as directed by an RNA template. The secondary structure is the local topology, the helices and strands, and this forms mostly because of the release of energy through the formation of hydrogen bonds. The tertiary structure is the actual fold of the protein, the way helices, strands, and loops are...	nmrlearner	News from NMR blogs	0	08-22-2010 01:58 AM
[NMR paper] Structural description of acid-denatured cytochrome c by hydrogen exchange and 2D NMR Structural description of acid-denatured cytochrome c by hydrogen exchange and 2D NMR. Related Articles Structural description of acid-denatured cytochrome c by hydrogen exchange and 2D NMR. Biochemistry. 1990 Nov 20;29(46):10433-7 Authors: Jeng MF, Englander SW, Elöve GA, Wand AJ, Roder H Hydrogen exchange and two-dimensional nuclear magnetic resonance (2D NMR) techniques were used to characterize the structure of oxidized horse cytochrome c at acid pH and high ionic strength. Under these conditions, cytochrome c is known to assume a...	nmrlearner	Journal club	0	08-21-2010 11:04 PM
[NMR paper] Denatured states of human carbonic anhydrase II: an NMR study of hydrogen/deuterium e Denatured states of human carbonic anhydrase II: an NMR study of hydrogen/deuterium exchange at tryptophan-indole-H(N) sites. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Denatured states of human carbonic anhydrase II: an NMR study of hydrogen/deuterium exchange at tryptophan-indole-H(N) sites. FEBS Lett. 1999 Feb 26;445(2-3):361-5 Authors: Jonasson P, Kjellsson A, Sethson I, Jonsson BH Hydrogen/deuterium (H/D) exchange measurements in low and moderate...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off