Related ArticlesHydration of the oxygen-evolving complex of photosystem II probed in the dark-stable S1 state using proton NMR dispersion profiles.
Phys Chem Chem Phys. 2014 Jun 28;16(24):11924-35
Authors: Han G, Huang Y, Koua FH, Shen JR, Westlund PO, Messinger J
Abstract
The hydration of the oxygen-evolving complex (OEC) was characterized in the dark stable S1 state of photosystem II using water R1(?) NMR dispersion (NMRD) profiles. The R1(?) NMRD profiles were recorded over a frequency range from 0.01 MHz to 40 MHz for both intact and Mn-depleted photosystem II core complexes from Thermosynechococcus vulcanus (T. vulcanus). The intact-minus-(Mn)-depleted difference NMRD profiles show a characteristic dispersion from approximately 0.03 MHz to 1 MHz, which is interpreted on the basis of the Solomon-Bloembergen-Morgan (SBM) and the slow motion theories as being due to a paramagnetic enhanced relaxation (PRE) of water protons. Both theories are qualitatively consistent with the ST = 1, g = 4.9 paramagnetic state previously described for the S1 state of the OEC; however, an alternative explanation involving the loss of a separate class of long-lived internal waters due to the Mn-depletion procedure can presently not be ruled out. Using a point-dipole approximation the PRE-NMRD effect can be described as being caused by 1-2 water molecules that are located about 10 Å away from the spin center of the Mn4CaO5 cluster in the OEC. The application of the SBM theory to the dispersion observed for PSII in the S1 state is questionable, because the parameters extracted do not fulfil the presupposed perturbation criterion. In contrast, the slow motion theory gives a consistent picture indicating that the water molecules are in fast chemical exchange with the bulk (?w < 1 ?s). The modulation of the zero-field splitting (ZFS) interaction suggests a (restricted) reorientation/structural equilibrium of the Mn4CaO5 cluster with a characteristic time constant of ?ZFS = 0.6-0.9 ?s.
[NMR paper] Mechanism of E-cadherin dimerization probed by NMR relaxation dispersion.
Mechanism of E-cadherin dimerization probed by NMR relaxation dispersion.
Mechanism of E-cadherin dimerization probed by NMR relaxation dispersion.
Proc Natl Acad Sci U S A. 2013 Sep 25;
Authors: Li Y, Altorelli NL, Bahna F, Honig B, Shapiro L, Palmer AG
Abstract
Epithelial cadherin (E-cadherin), a member of the classical cadherin family, mediates calcium-dependent homophilic cell-cell adhesion. Crystal structures of classical cadherins reveal an adhesive dimer interface featuring reciprocal exchange of N-terminal ?-strands between two...
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[NMR paper] Detection of the water-binding sites of the oxygen-evolving complex of Photosystem II using W-band 17O electron-electron double resonance-detected NMR spectroscopy.
Detection of the water-binding sites of the oxygen-evolving complex of Photosystem II using W-band 17O electron-electron double resonance-detected NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Detection of the water-binding sites of the oxygen-evolving complex of Photosystem II using W-band 17O electron-electron double resonance-detected NMR spectroscopy.
J Am Chem Soc. 2012 Oct 10;134(40):16619-34
Authors: Rapatskiy L, Cox N, Savitsky A, Ames WM, Sander J,...
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Detection of the Water-BindingSites of the Oxygen-EvolvingComplex of Photosystem II Using W-Band 17O Electron–ElectronDouble Resonance-Detected NMR Spectroscopy
Detection of the Water-BindingSites of the Oxygen-EvolvingComplex of Photosystem II Using W-Band 17O Electron–ElectronDouble Resonance-Detected NMR Spectroscopy
Leonid Rapatskiy, Nicholas Cox, Anton Savitsky, William M. Ames, Julia Sander, Marc. M. Nowaczyk, Matthias Ro?gner, Alain Boussac, Frank Neese, Johannes Messinger and Wolfgang Lubitz
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja3053267/aop/images/medium/ja-2012-053267_0012.gif
Journal of the American Chemical Society
DOI: 10.1021/ja3053267...
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Conformational dynamics of recoverin's Ca(2+) -myristoyl switch probed by (15) N NMR relaxation dispersion and chemical shift analysis.
Conformational dynamics of recoverin's Ca(2+) -myristoyl switch probed by (15) N NMR relaxation dispersion and chemical shift analysis.
Conformational dynamics of recoverin's Ca(2+) -myristoyl switch probed by (15) N NMR relaxation dispersion and chemical shift analysis.
Proteins. 2011 Feb 16;
Authors: Xu X, Ishima R, Ames JB
Recoverin, a member of the neuronal calcium sensor (NCS) branch of the calmodulin superfamily, serves as a calcium sensor in retinal rod cells. Ca(2+) -induced conformational changes in recoverin promote extrusion of its...
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04-06-2011 10:54 AM
Solid-state (55)Mn NMR spectroscopy of bis(?-oxo)dimanganese(IV) [Mn(2)O(2)(salpn)(2)], a model for the oxygen evolving complex in photosystem II.
Solid-state (55)Mn NMR spectroscopy of bis(?-oxo)dimanganese(IV) , a model for the oxygen evolving complex in photosystem II.
Solid-state (55)Mn NMR spectroscopy of bis(?-oxo)dimanganese(IV) , a model for the oxygen evolving complex in photosystem II.
J Am Chem Soc. 2010 Dec 1;132(47):16727-9
Authors: Ellis PD, Sears JA, Yang P, Dupuis M, Boron TT, Pecoraro VL, Stich TA, Britt RD, Lipton AS
We have examined the antiferromagneticly coupled bis(?-oxo)dimanganese(IV) complex (1) with (55)Mn solid-state NMR at cryogenic temperatures and...
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03-13-2011 04:01 AM
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy.
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy.
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy.
Methods Enzymol. 2010;478:305-22
Authors: Yamaguchi Y, Kato K
Unique advantages offered by nuclear magnetic resonance (NMR) spectroscopy provide high-resolution information not only on structures but also on dynamics and interactions of glycoconjugates in solution. These benefits are further enhanced by applying stable-isotope-labeling techniques, which we...
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[NMR paper] NMR analysis of the transient complex between membrane photosystem I and soluble cyto
NMR analysis of the transient complex between membrane photosystem I and soluble cytochrome c6.
Related Articles NMR analysis of the transient complex between membrane photosystem I and soluble cytochrome c6.
J Biol Chem. 2005 Mar 4;280(9):7925-31
Authors: Díaz-Moreno I, Díaz-Quintana A, Molina-Heredia FP, Nieto PM, Hansson O, De la Rosa MA, Karlsson BG
A structural analysis of the surface areas of cytochrome c(6), responsible for the transient interaction with photosystem I, was performed by NMR transverse relaxation-optimized spectroscopy....
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[NMR paper] The Aspergillus nidulans transcription factor AlcR forms a stable complex with its ha
The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study.
FEBS Lett. 1997 May 19;408(2):235-40
Authors: Cerdan R, Collin D, Lenouvel F, Felenbok B, Guittet E
The Aspergillus nidulans transcription factor AlcR is shown by NMR and gel retardation assay to form a...