A calibration-based approach to real-time in-vivo monitoring of pyruvate C1 and C2 polarization using the J_CC spectral asymmetry #DNPNMR
From The DNP-NMR Blog:
A calibration-based approach to real-time in-vivo monitoring of pyruvate C1 and C2 polarization using the J_CC spectral asymmetry #DNPNMR
To measure the degree of polarization induced by the DNP process, one possibility is to calculate the ratio of the microwave OFF signal to microwave ON signal (neglecting depolarization under MAS conditions). While the ON signal is easy to measure since it typically a much better signal-to-noise ratio, measuring the OFF signal can often be challenging and the quality of the OFF signal will determine the error.
Measuring the...
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05-18-2020 09:18 PM
[NMR paper] Cell-free protein synthesis enhancement from real-time NMR metabolite kinetics: redirecting energy fluxes in hybrid RRL systems.
Cell-free protein synthesis enhancement from real-time NMR metabolite kinetics: redirecting energy fluxes in hybrid RRL systems.
Related Articles Cell-free protein synthesis enhancement from real-time NMR metabolite kinetics: redirecting energy fluxes in hybrid RRL systems.
ACS Synth Biol. 2017 Sep 15;:
Authors: Panthu B, Ohlmann T, Perrier J, Schlattner U, Jalinot P, Elena-Herrmann B, Rautureau GJP
Abstract
A counter-intuitive cell-free protein synthesis (CFPS) strategy, based on reducing the ribosomal fraction in rabbit...
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09-16-2017 09:58 PM
[NMR paper] Real-Time Analysis of Folding upon Binding of a Disordered Protein by using Dissolution DNP NMR Spectroscopy.
Real-Time Analysis of Folding upon Binding of a Disordered Protein by using Dissolution DNP NMR Spectroscopy.
Related Articles Real-Time Analysis of Folding upon Binding of a Disordered Protein by using Dissolution DNP NMR Spectroscopy.
Angew Chem Int Ed Engl. 2017 May 16;:
Authors: Ragavan M, Iconaru LI, Park CG, Kriwacki RW, Hilty C
Abstract
The kinase inhibitory domain of the cell cycle regulatory protein p27(Kip1) (p27) was nuclear spin hyperpolarized using dissolution dynamic nuclear polarization (D-DNP). While...
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05-17-2017 12:46 PM
[NMR paper] A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time.
A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time.
J Am Chem Soc. 2015 May 27;137(20):6468-71
Authors: Thongwichian R, Kosten J, Benary U, Rose HM, Stuiver M, Theillet FX, Dose A, Koch B, Yokoyama H, Schwarzer D, Wolf J, Selenko P
Abstract
Cell...
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03-02-2016 07:20 PM
A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time
A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time
Rossukon Thongwichian, Jonas Kosten, Uwe Benary, Honor May Rose, Marchel Stuiver, Francois-Xavier Theillet, Alexander Dose, Birgit Koch, Hideki Yokoyama, Dirk Schwarzer, Jana Wolf and Philipp Selenko
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b02987/20150515/images/medium/ja-2015-029876_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.5b02987
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA ...
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05-16-2015 01:45 PM
Real-time pure shift 15 N HSQC of proteins: a real improvement in resolution and sensitivity
Real-time pure shift 15 N HSQC of proteins: a real improvement in resolution and sensitivity
Abstract
Spectral resolution in proton NMR spectroscopy is reduced by the splitting of resonances into multiplets due to the effect of homonuclear scalar couplings. Although these effects are often hidden in protein NMR spectroscopy by low digital resolution and routine apodization, behind the scenes homonuclear scalar couplings increase spectral overcrowding. The possibilities for biomolecular NMR offered by new pure shift NMR methods are illustrated here....
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03-04-2015 08:56 AM
[NMR paper] Object-oriented approach to drug design enabled by NMR SOLVE: first real-time structu
Object-oriented approach to drug design enabled by NMR SOLVE: first real-time structural tool for characterizing protein-ligand interactions.
Related Articles Object-oriented approach to drug design enabled by NMR SOLVE: first real-time structural tool for characterizing protein-ligand interactions.
J Cell Biochem Suppl. 2001;Suppl 37:99-105
Authors: Sem DS, Yu L, Coutts SM, Jack R
As a result of genomics efforts, the number of protein drug targets is expected to increase by an order of magnitude. Functional genomics efforts are identifying...
A Hybrid Orbitrap-Nanoelectromechanical Systems Approach for the Analysis of Individual, Intact Proteins in Real Time
Linear Mode
