Chromatin, a dynamic protein-DNA complex that regulates eukaryotic genome accessibility and essential functions, is composed of nucleosomes connected by linker DNA with each nucleosome consisting of DNA wrapped around an octamer of histones H2A, H2B, H3 and H4. Magic angle spinning solid-state nuclear magnetic resonance (NMR) spectroscopy can yield unique insights into histone structure and dynamics in condensed nucleosomes and nucleosome arrays representative of chromatin at physiological...
[NMR paper] DNP-enhanced solid-state NMR spectroscopy of chromatin polymers
DNP-enhanced solid-state NMR spectroscopy of chromatin polymers
Chromatin is a DNA-protein polymer that represents the functional form of the genome. The main building block of chromatin is the nucleosome, a structure that contains 147 base pairs of DNA and two copies each of the histone proteins H2A, H2B, H3 and H4. Previous work has shown that magic angle spinning (MAS) NMR spectroscopy can capture the nucleosome at high resolution although studies have been challenging due to low sensitivity, the presence of dynamic and rigid components, and the complex...
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06-17-2022 08:24 AM
[NMR paper] Emerging Contributions of Solid-State NMR Spectroscopy to Chromatin Structural Biology
Emerging Contributions of Solid-State NMR Spectroscopy to Chromatin Structural Biology
The eukaryotic genome is packaged into chromatin, a polymer of DNA and histone proteins that regulates gene expression and the spatial organization of nuclear content. The repetitive character of chromatin is diversified into rich layers of complexity that encompass DNA sequence, histone variants and post-translational modifications. Subtle molecular changes in these variables can often lead to global chromatin rearrangements that dictate entire gene programs with far reaching implications for...
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10-29-2021 10:57 AM
[NMR paper] Bacteriophage Tail-Tube Assembly Studied by Proton-Detected 4D Solid-State NMR
Bacteriophage Tail-Tube Assembly Studied by Proton-Detected 4D Solid-State NMR
Obtaining unambiguous resonance assignments remains a major bottleneck in solid-state NMR studies of protein structure and dynamics. Particularly for supramolecular assemblies with large subunits (>150 residues), the analysis of crowded spectral data presents a challenge, even if three-dimensional (3D) spectra are used. Here, we present a proton-detected 4D solid-state NMR assignment procedure that is tailored for large assemblies. The key to recording 4D spectra with three indirect carbon or nitrogen...
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07-10-2017 02:13 PM
[NMR paper] Bacteriophage Tail Tube Assembly Studied by Proton-Detected 4D Solid-State NMR.
Bacteriophage Tail Tube Assembly Studied by Proton-Detected 4D Solid-State NMR.
Related Articles Bacteriophage Tail Tube Assembly Studied by Proton-Detected 4D Solid-State NMR.
Angew Chem Int Ed Engl. 2017 Jun 23;:
Authors: Zinke M, Fricke P, Samson C, Hwang S, Wall J, Lange S, Zinn-Justin S, Lange A
Abstract
Obtaining unambiguous resonance assignments remains a major bottleneck in solid-state NMR studies of protein structure and dynamics. Particularly for supramolecular assemblies with large subunits (>150 residues), the...
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06-24-2017 08:20 PM
[NMR paper] Bacteriophage Tail Tube Assembly Studied by Proton-Detected 4D Solid-State NMR
Bacteriophage Tail Tube Assembly Studied by Proton-Detected 4D Solid-State NMR
Obtaining unambiguous resonance assignments remains a major bottleneck in solid-state NMR studies of protein structure and dynamics. Particularly for supramolecular assemblies with large subunits (>150 residues), the analysis of crowded spectral data presents a challenge, even if three-dimensional (3D) spectra are used. Here, we present a proton-detected 4D solid-state NMR assignment procedure that is tailored for large assemblies. The key to recording 4D spectra with three indirect carbon or nitrogen...
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06-23-2017 04:36 PM
StructureElucidation of Mixed-Linker Zeolitic ImidazolateFrameworks by Solid-State 1H CRAMPS NMR Spectroscopy andComputational Modeling
StructureElucidation of Mixed-Linker Zeolitic ImidazolateFrameworks by Solid-State 1H CRAMPS NMR Spectroscopy andComputational Modeling
Krishna C. Jayachandrababu, Ross J. Verploegh, Johannes Leisen, Ryan C. Nieuwendaal, David S. Sholl and Sankar Nair
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.6b02754/20160602/images/medium/ja-2016-02754g_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.6b02754
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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06-03-2016 03:35 AM
[NMR paper] Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR.
Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR.
Related Articles Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR.
Biochemistry. 2014 Oct 3;
Authors: Hoop CL, Lin HK, Kar K, Hou Z, Poirier MA, Wetzel R, van der Wel PC
Abstract
In Huntington's Disease (HD), expansion of a polyglutamine (polyQ) domain in the huntingtin (htt) protein leads to misfolding and aggregation....
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10-04-2014 05:16 PM
[NMR paper] The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged he
The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged helix-turn-helix fold as determined by NMR spectroscopy.
Related Articles The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged helix-turn-helix fold as determined by NMR spectroscopy.
Nucleic Acids Res. 2003 Dec 15;31(24):7199-207
Authors: Ono K, Kusano O, Shimotakahara S, Shimizu M, Yamazaki T, Shindo H
Hho1p is assumed to serve as a linker histone in Saccharomyces cerevisiae and, notably, it possesses two putative globular...
Histone H3 core domain in chromatin with different DNA linker lengths studied by 1H-Detected solid-state NMR spectroscopy
Linear Mode
