Related ArticlesHighly selective excitation in biomolecular NMR by frequency-switched single-transition cross-polarization.
J Am Chem Soc. 2002 Mar 13;124(10):2076-7
Authors: Ferrage F, Eykyn TR, Bodenhausen G
A new method for selective excitation in biomolecular NMR uses two-fold single-transition cross-polarization between protons and nitrogen-15 or carbon-13 nuclei. Switching the frequencies between the forward and backward transfer steps allows one to select a multiplet pattern that is associated with a single pair of spins in a medium-size protein. The efficiency of the transfer benefits from so-called TROSY line-narrowing effects which arise from interference between relaxation mechanisms.
NMR Excitation, Dephasing and Spin Echoes
NMR Excitation, Dephasing and Spin Echoes
http://i.ytimg.com/vi/KtWnmFg-u5g/default.jpg
NMR Excitation, Dephasing and Spin Echoes
This short animation shows the process of NMR excitation in the laboratory and the rotating frame, as well as the dephasing that occurs from field inhomogeneity and the formation of the Hahn spin echo. Please credit (c)2010 Mark Cohen (mscohen@ucla.edu) during re-use.
From:markcat3t
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http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif...
nmrlearner
NMR educational videos
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01-29-2012 07:45 PM
Nonselective excitation of pulsed ELDOR using multi-frequency microwaves
Nonselective excitation of pulsed ELDOR using multi-frequency microwaves
Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 17 September 2011</br>
Yuki*Asada, Risa*Mutoh, Masahiro*Ishiura, Hiroyuki*Mino</br>
The use of a polychromatic microwave pulse to expand the pumping bandwidth in pulsed electron-electron double resonance (PELDOR) was investigated. The pumping pulse was applied in resonance with the broad (~100 mT) electron paramagnetic resonance (EPR) signal of the manganese cluster of photosystem II in the S2state. The observation pulses were in...
nmrlearner
Journal club
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09-23-2011 05:53 AM
Two-photon excitation in nuclear magnetic and quadrupole resonance
Two-photon excitation in nuclear magnetic and quadrupole resonance
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 56, Issue 3, April 2010, Pages 232-246</br>
P.T.*Eles, C.A.*Michal</br>
More...
nmrlearner
Journal club
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09-13-2011 09:15 PM
Frequency-selective heteronuclear dephasing and selective carbonyl labeling to deconvolute crowded spectra of membrane proteins by magic angle spinning NMR.
Frequency-selective heteronuclear dephasing and selective carbonyl labeling to deconvolute crowded spectra of membrane proteins by magic angle spinning NMR.
Frequency-selective heteronuclear dephasing and selective carbonyl labeling to deconvolute crowded spectra of membrane proteins by magic angle spinning NMR.
J Magn Reson. 2011 Mar 17;
Authors: Traaseth NJ, Veglia G
We present a new method that combines carbonyl-selective labeling with frequency-selective heteronuclear recoupling to resolve the spectral overlap of magic angle spinning (MAS) NMR...
nmrlearner
Journal club
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04-13-2011 11:57 PM
Frequency-Selective Heteronuclear Dephasing and Selective Carbonyl Labeling to Deconvolute Crowded Spectra of Membrane Proteins By Magic Angle Spinning NMR
Frequency-Selective Heteronuclear Dephasing and Selective Carbonyl Labeling to Deconvolute Crowded Spectra of Membrane Proteins By Magic Angle Spinning NMR
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 17 March 2011</br>
Nathaniel J., Traaseth , Gianluigi, Veglia</br>
We present a new method that combines carbonyl-selective labeling with frequency-selective heteronuclear recoupling to resolve the spectral overlap of magic angle spinning (MAS) NMR spectra of membrane proteins in fluid lipid membranes with broad lines and...
nmrlearner
Journal club
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03-18-2011 06:43 AM
[U. of Ottawa NMR Facility Blog] Excitation Profiles for Shaped Pulses
Excitation Profiles for Shaped Pulses
Shaped pulses are very commonly used for selective excitation and nonselective inversion in a large number of NMR pulse sequences. The frequency domain excitation profile of a radio frequency pulse is the Fourier transform of the time dependent pulse shape and determines the width, uniformity and phase of the frequency spectrum excited. Since time and frequency are reciprocals of one another, short rf pulses have very wide excitation profiles and long rf pulses have very narrow selective excitation profiles. In a previous BLOG post the excitation...
nmrlearner
News from NMR blogs
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01-21-2011 03:31 AM
[NMR paper] Development of NMR instrumentation to achieve excitation of large bandwidths in high-
Development of NMR instrumentation to achieve excitation of large bandwidths in high-resolution spectra at high field.
Related Articles Development of NMR instrumentation to achieve excitation of large bandwidths in high-resolution spectra at high field.
J Magn Reson. 2001 Jun;150(2):161-6
Authors: Luchinat C, Piccioli M, Pierattelli R, Engelke F, Marquardsen T, Ruin R
A prototype 2.5-mm (1)H high-resolution probe for an 18.8-T (800 MHz) nuclear magnetic resonance spectrometer has been designed, together with a dedicated amplifier capable of...
nmrlearner
Journal club
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11-19-2010 08:32 PM
[U. of Ottawa NMR Facility Blog] Gradient Spin Echoes for Selective Excitation
Gradient Spin Echoes for Selective Excitation
Shaped excitation pulses can replace the non-selective hard pulses typically used in a one-pulse measurement to achieve selective excitation. Another method of achieving selective excitation is the gradient spin echo using a selective 180° pulse. This technique is demonstrated in the figure below. http://4.bp.blogspot.com/_5wBTR2kKTqA/S_UxeG5oXdI/AAAAAAAAAzc/BHWef-Tse7s/s400/grad_spin_echo.jpgA non-selective hard 90°x pulse is first given followed by a pair of identical pulsed field gradients sandwiching a soft selective 180° pulse about the y...
Highly selective excitation in biomolecular NMR by frequency-switched single-transiti
Linear Mode
