NMR paper Highly selective excitation in biomolecular NMR by frequency-switched single-transiti

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[NMR paper] Highly selective excitation in biomolecular NMR by frequency-switched single-transiti

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NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

Refinement:

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Structure from chemical shifts:

Fragment-based:

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Torsion angles from chemical shifts:

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Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

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Interactions from chemical shifts:

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Methyl S2

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Shiftx2

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Proshift

PPM

CheShift-2- Cα

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Shifty

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Poulsen_rc_CS

Disordered proteins:

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11-24-2010, 08:49 PM

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Highly selective excitation in biomolecular NMR by frequency-switched single-transiti

Highly selective excitation in biomolecular NMR by frequency-switched single-transition cross-polarization.

Related Articles Highly selective excitation in biomolecular NMR by frequency-switched single-transition cross-polarization.

J Am Chem Soc. 2002 Mar 13;124(10):2076-7

Authors: Ferrage F, Eykyn TR, Bodenhausen G

A new method for selective excitation in biomolecular NMR uses two-fold single-transition cross-polarization between protons and nitrogen-15 or carbon-13 nuclei. Switching the frequencies between the forward and backward transfer steps allows one to select a multiplet pattern that is associated with a single pair of spins in a medium-size protein. The efficiency of the transfer benefits from so-called TROSY line-narrowing effects which arise from interference between relaxation mechanisms.

PMID: 11878936 [PubMed - indexed for MEDLINE]

Source: PubMed

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