During therapeutic protein development, two-dimensional (2D) heteronuclear NMR spectra can be a powerful analytical method for measuring protein higher order structure (HOS) in solution since the spectra exhibit much higher resolution than homonuclear 1H spectra. However, 2D NMR capabilities for characterizing protein HOS in crystalline states remain to be assessed, given the low 13C natural abundance and intrinsically broader lines in solid-state NMR (SSNMR). Herein, high-resolution...
[NMR paper] NMR Spectroscopy for Protein Higher Order Structure Similarity Assessment in Formulated Drug Products
NMR Spectroscopy for Protein Higher Order Structure Similarity Assessment in Formulated Drug Products
Peptide and protein drug molecules fold into higher order structures (HOS) in formulation and these folded structures are often critical for drug efficacy and safety. Generic or biosimilar drug products (DPs) need to show similar HOS to the reference product. The solution NMR spectroscopy is a non-invasive, chemically and structurally specific analytical method that is ideal for characterizing protein therapeutics in formulation. However, only limited NMR studies have been performed...
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Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review
Quality assessment of biologics: higher order structure analysis using NMR European Pharmaceutical ReviewThe webinar will describe how 2D NMR can offer protein biochemists unprecedented analytical precision. By assigning just one spectrum, users can obtain a ...
Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review
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01-07-2019 05:49 AM
[NMR paper] Application of 2D-NMR with room temperature NMR probes for the assessment of the higher order structure of filgrastim.
Application of 2D-NMR with room temperature NMR probes for the assessment of the higher order structure of filgrastim.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Application of 2D-NMR with room temperature NMR probes for the assessment of the higher order structure of filgrastim.
J Pharm Biomed Anal. 2017 Jul 15;141:229-233
Authors: Brinson RG, Ghasriani H, Hodgson DJ, Adams KM, McEwen I, Freedberg DI, Chen K, Keire DA, Aubin Y, Marino JP
...
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01-26-2018 12:08 PM
[NMR paper] Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
Related Articles Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
J Pharm Biomed Anal. 2016 Jun 7;128:398-407
Authors: Chen K, Long DS, Lute SC, Levy MJ, Brorson KA, Keire DA
Abstract
Monoclonal antibody (mAb) drugs constitute the largest class of protein therapeutics currently on the market. Correctly folded protein higher order structure...
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06-28-2016 06:25 PM
[NMR paper] On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods.
On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods.
Related Articles On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods.
Anal Chem. 2015 Apr 30;
Authors: Poppe L, Jordan JB, Rogers G, Schnier PD
Abstract
An important aspect in the analytical characterization of protein therapeutics is the comprehensive characterization of higher...
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05-02-2015 09:41 PM
Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy
From The DNP-NMR Blog:
Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy
Debelouchina, G.T., et al., Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy. J Am Chem Soc, 2013. 135(51): p. 19237-47.
http://www.ncbi.nlm.nih.gov/pubmed/24304221
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01-27-2014 09:59 PM
[NMR paper] Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy.
Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy.
Related Articles Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy.
J Am Chem Soc. 2013 Dec 4;
Authors: Debelouchina GT, Bayro MJ, Fitzpatrick AW, Ladizhansky V, Colvin MT, Caporini MA, Jaroniec CP, Bajaj VS, Rosay MM, Macphee CE, Vendruscolo M, Maas WE, Dobson CM, Griffin RG
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Protein magic angle spinning (MAS) NMR spectroscopy has generated structural models of several amyloid fibril systems, thus providing valuable information regarding the...
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Journal Highlight: Assessment of higher order structure comparability in ... - spectroscopyNOW.com
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Journal Highlight: Assessment of higher order structure comparability in ...
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Abstract: In this work, we applied nuclear magnetic resonance (NMR) spectroscopy to rapidly assess higher order structure (HOS) comparability in protein samples. Using a variation of the NMR fingerprinting approach described by Panjwani et al.
Journal Highlight: Assessment of higher order structure comparability in ... - spectroscopyNOW.com
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Higher Order Structure Differences Among Insulin Crystalline Drugs Revealed by 2D heteronuclear NMR
Linear Mode
