NMR paper High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.

		BioNMR > Educational resources > Journal club
[NMR paper] High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-01-2017, 11:41 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,778

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.

High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.

High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.

Anal Chem. 2017 Jun 30;:

Authors: Morimoto D, Walinda E, Iwakawa N, Nishizawa M, Kawata Y, Yamamoto A, Shirakawa M, Scheler U, Sugase K

Abstract
Shear stress can induce structural deformation of proteins, which might result in aggregate formation. Rheo-NMR spectroscopy has the potential to monitor structural changes in proteins under shear stress at the atomic level; however, existing Rheo-NMR methodologies have insufficient sensitivity to probe protein structure and dynamics. Here we present a simple and versatile approach to Rheo-NMR, which maximizes sensitivity by using a spectrometer equipped with a cryogenic probe. As a result, the sensitivity of the instrument ranks highest among the Rheo-NMR spectrometers reported so far. We demonstrate that the newly developed Rheo-NMR instrument can acquire high-quality relaxation data for a protein under shear stress and can trace structural changes in a protein during fibril formation in real time. The described approach will facilitate rheological studies on protein structural deformation, thereby aiding a physical understanding of shear-induced amyloid fibril formation.

PMID: 28665116 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe Abstract Field-dependent NMR studies of bio-molecular systems using a sample shuttling hardware operating on a high-field NMR apparatus have provided valuable structural and dynamic information. We have recently published a design of a compact sample transportation device, called â??field-cyclerâ??, which was installed in a commercial spectrometer and which provided highly precise positioning and stability during high speed...	nmrlearner	Journal club	0	11-19-2016 08:35 PM
[NMR paper] Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR. Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR. Related Articles Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR. J Biomol NMR. 2015 Oct 23; Authors: Takeuchi K, Arthanari H, Shimada I, Wagner G Abstract Detection of (15)N in multidimensional NMR experiments of proteins has sparsely been utilized because of the low gyromagnetic ratio (?) of nitrogen and the presumed low sensitivity of such experiments. Here we show that selecting the TROSY...	nmrlearner	Journal club	0	10-27-2015 12:33 PM
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR Abstract Detection of 15N in multidimensional NMR experiments of proteins has sparsely been utilized because of the low gyromagnetic ratio (Î³) of nitrogen and the presumed low sensitivity of such experiments. Here we show that selecting the TROSY components of proton-attached 15N nuclei (TROSY 15NH) yields high quality spectra in high field magnets (>600Â*MHz) by taking advantage of the slow 15N transverse relaxation and compensating for the inherently low...	nmrlearner	Journal club	0	10-24-2015 05:49 AM
R&D - PhD - Scientist - Rheo-NMR \| Procter & Gamble R&D - PhD - Scientist - Rheo-NMR \| Procter & Gamble US - OH - Cincinnati, The successful candidate must have a Ph.D. degree based on research demonstrating in-depth knowledge of NMR principles. She or he should have technical depth in both NMR theory and experimentation. Th More...	nmrlearner	Job marketplace	0	10-21-2014 11:31 PM
Practical aspects of high-sensitivity multidimensional 13C MAS NMR spectroscopy of perdeuterated proteins Practical aspects of high-sensitivity multidimensional 13C MAS NMR spectroscopy of perdeuterated proteins April 2012 Publication year: 2012 Source:Journal of Magnetic Resonance, Volume 217</br> </br> The double nucleus enhanced recoupling (DONER) experiment employs simultaneous irradiation of protons and deuterons to promote spin diffusion processes in a perdeuterated protein. This results in 4–5times higher sensitivity in 2D 13C–13C correlation experiments as compared to PDSD . Here, a quantitative comparison of PDSD, 1H-DARR, 2H-DARR, and 1H+ 2H DONER has been...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Postdoc in Soft Matter and Rheo-NMR Postdoc in Soft Matter and Rheo-NMR We seek a PhD graduate or equivalent in Physics with a strong background in soft matter. The focus will be on the behavior of complex fluids, shear banding, temporal-spatial fluctuations as well as start up flow. The position is for one year (with the option to extend by 8 months, depending on performance), based in Wellington New Zealand. An additional background in NMR including Rheo-NMR is desirable. More...	nmrlearner	General	0	06-08-2012 08:47 PM
Practical Aspects of High-Sensitivity Multidimensional 13C MAS NMR Spectroscopy of Perdeuterated Proteins Practical Aspects of High-Sensitivity Multidimensional 13C MAS NMR Spectroscopy of Perdeuterated Proteins Publication year: 2012 Source:Journal of Magnetic Resonance</br> Ümit Akbey, Barth-Jan van Rossum, Hartmut Oschkinat</br> The double nucleus enhanced recoupling (DONER) experiment employs simultaneous irradiation of protons and deuterons to promote spin diffusion processes in a perdeuterated protein. This results in 4-5 times higher sensitivity in 2D 13C-13C correlation experiments as compared to PDSD. Here, a quantitative comparison of PDSD, 1H-DARR, 2H-DARR, and...	nmrlearner	Journal club	0	03-09-2012 09:16 AM
Practical Aspects of High-Sensitivity MultidimensionalC MAS NMR Spectroscopy of Perdeuterated Proteins Practical Aspects of High-Sensitivity MultidimensionalC MAS NMR Spectroscopy of Perdeuterated Proteins Publication year: 2012 Source: Journal of Magnetic Resonance, Available online 1 March 2012</br> ÜmitAkbey, Barth-Janvan Rossum, Hartmut*Oschkinat</br> Thedouble nucleus enhanced recoupling(DONER) experiment employs simultaneous irradiation of protons and deuterons to promote spin diffusion processes in a perdeuterated protein. This results in 4-5 times higher sensitivity in 2DC-C correlation experiments as compared to PDSD.Here, a quantitative comparison of PDSD,H-DARR,H-DARR,...	nmrlearner	Journal club	0	03-01-2012 11:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off