Related ArticlesHigh-resolution Solid-state NMR Characterization of Ligand Binding to a Protein Immobilized in a Silica Matrix.
J Phys Chem B. 2017 Aug 01;:
Authors: Cerofolini L, Giuntini S, Louka A, Ravera E, Fragai M, Luchinat C
Abstract
Solid state NMR is becoming a powerful tool to detect atomic-level structural features of biomolecules even when they are bound to - or trapped in - solid systems that lack long-range three dimensional order. We here demonstrate that it is possible to probe protein-ligand interactions from a protein-based perspective also when the protein is entrapped in silica, thus translating into biomolecular solid state NMR all the considerations that are usually made to understand the chemical nature of the interaction of a protein with its ligands. This work provides the proof of concept that also immobilized enzymes can be used for protein-based NMR protein-ligand interactions for drug discovery.
PMID: 28762736 [PubMed - as supplied by publisher]
[NMR paper] Ubiquitin immobilized on mesoporous MCM41 silica surfaces - Analysis by solid-state NMR with biophysical and surface characterization.
Ubiquitin immobilized on mesoporous MCM41 silica surfaces - Analysis by solid-state NMR with biophysical and surface characterization.
Related Articles Ubiquitin immobilized on mesoporous MCM41 silica surfaces - Analysis by solid-state NMR with biophysical and surface characterization.
Biointerphases. 2017 May 31;12(2):02D414
Authors: Adiram-Filiba N, Schremer A, Ohaion E, Nadav-Tsubery M, Lublin-Tennenbaum T, Keinan-Adamsky K, Goobes G
Abstract
Deriving the conformation of adsorbed proteins is important in the assessment of...
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[NMR paper] (1)H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization.
(1)H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif Related Articles (1)H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization.
Sci Rep. 2016;6:27851
Authors: Ravera E, Cerofolini L, Martelli T, Louka A, Fragai M, Luchinat C
Abstract
Proton-detection in solid-state NMR, enabled by high magnetic...
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[NMR paper] Characterization of the Vibrio cholerae Extracellular Matrix: A Top-Down Solid-State NMR Approach.
Characterization of the Vibrio cholerae Extracellular Matrix: A Top-Down Solid-State NMR Approach.
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Biochim Biophys Acta. 2014 Jun 6;
Authors: Reichhardt C, Fong JC, Yildiz F, Cegelski L
Abstract
Bacterial biofilms are communities of bacterial cells surrounded by a self-secreted extracellular matrix. Biofilm formation by Vibrio cholerae, the human pathogen responsible for cholera, contributes to its environmental...
[NMR paper] Resolution and measurement of heteronuclear dipolar couplings of a noncrystalline protein immobilized in a biological supramolecular assembly by proton-detected MAS solid-state NMR spectroscopy
Resolution and measurement of heteronuclear dipolar couplings of a noncrystalline protein immobilized in a biological supramolecular assembly by proton-detected MAS solid-state NMR spectroscopy
Publication date: Available online 26 October 2013
Source:Journal of Magnetic Resonance</br>
Author(s): Sang Ho Park , Chen Yang , Stanley J. Opella , Leonard J. Mueller</br>
Two-dimensional 15N chemical shift/1H chemical shift and three-dimensional 1H-15N dipolar coupling/15N chemical shift/1H chemical shift MAS solid-state NMR correlation spectra of the filamentous...
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[NMR paper] Investigation of ligand-receptor systems by high-resolution solid-state NMR: recent p
Investigation of ligand-receptor systems by high-resolution solid-state NMR: recent progress and perspectives.
Related Articles Investigation of ligand-receptor systems by high-resolution solid-state NMR: recent progress and perspectives.
Arch Pharm (Weinheim). 2005 Jun;338(5-6):217-28
Authors: Luca S, Heise H, Lange A, Baldus M
Solid-state Nuclear Magnetic Resonance (NMR) provides a general method to study molecular structure and dynamics in a non-crystalline and insoluble environment. We discuss the latest methodological progress to...
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11-25-2010 08:21 PM
[NMR paper] Characterization of protein-ligand interactions by high-resolution solid-state NMR sp
Characterization of protein-ligand interactions by high-resolution solid-state NMR spectroscopy.
Related Articles Characterization of protein-ligand interactions by high-resolution solid-state NMR spectroscopy.
J Am Chem Soc. 2004 Nov 3;126(43):13948-53
Authors: Zech SG, Olejniczak E, Hajduk P, Mack J, McDermott AE
A novel approach for detection of ligand binding to a protein in solid samples is described. Hydrated precipitates of the anti-apoptotic protein Bcl-xL show well-resolved (13)C-(13)C 2D solid-state NMR spectra that allow...
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[NMR paper] High resolution NMR for screening ligand/protein binding.
High resolution NMR for screening ligand/protein binding.
Related Articles High resolution NMR for screening ligand/protein binding.
Curr Opin Drug Discov Devel. 1999 Jul;2(4):396-400
Authors: Shapiro MJ, Wareing JR
High resolution NMR spectroscopy has provided a versatile tool for assessing ligand/receptor interactions. NMR-based methods are currently being investigated which may prove valuable for compound screening. These techniques include the use of chemical shift perturbations, the monitoring of translational diffusion and the...
High-resolution Solid-state NMR Characterization of Ligand Binding to a Protein Immobilized in a Silica Matrix.
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