NMR paper High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

		BioNMR > Educational resources > Journal club
[NMR paper] High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 10:01 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

Related Articles High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

J Am Chem Soc. 2004 Oct 27;126(42):13765-77

Authors: Roberts MF, Redfield AG

We have used high-resolution field-cycling 31P NMR spectroscopy to measure spin-lattice relaxation rates (R1 = 1/T1) of multicomponent phospholipid vesicle and micelle samples over a large field range, from 0.1 to 11.7 T. The shape of the curve for R1 as a function of field and a model-free analysis were used to extract tauc, a correlation time for each type of phospholipid molecule in the bilayer that is likely to reflect rotation of the molecule about the axis perpendicular to the membrane surface; Sc2, a chemical shift anisotropy (CSA) order parameter; and tauhf, a time constant reflecting faster internal motion. This 31P technique was also used to monitor association of a peripheral membrane protein, Bacillus thuringiensis phosphatidylinositol-specific phospholipase C, with both phosphatidylcholine and phosphatidylmethanol bilayers. Differences in phospholipid dynamics induced by the protein shed light on how zwitterionic phosphatidylcholine, and not the anionic phosphatidylmethanol, activates the enzyme toward its substrate.

PMID: 15493936 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: Implications for Structure and Dynamics Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: Implications for Structure and Dynamics Publication year: 2012 Source: Journal of Magnetic Resonance, Available online 5 January 2012</br> Bernd*Reif</br> http://www.sciencedirect.com/cache/MiamiImageURL/1-s2.0-S1090780711005969-fx1.sml</br></br></br> Source: Journal of Magnetic Resonance	nmrlearner	Journal club	0	01-07-2012 03:12 PM
High-resolution NMR field-cycling device for full-range relaxation and structural studies of biopolymers on a shared commercial instrument High-resolution NMR field-cycling device for full-range relaxation and structural studies of biopolymers on a shared commercial instrument Abstract Improvements are described in a shuttling field-cycling device (Redfield in Magn Reson Chem 41:753â??768, 2003), designed to allow widespread access to this useful technique by configuring it as a removable module to a commercial 500 MHz NMR instrument. The main improvements described here, leading to greater versatility, high reliability and simple construction, include: shuttling provided by a linear motor driven by an integrated-control...	nmrlearner	Journal club	0	12-31-2011 10:40 AM
Field-cycling NMR relaxometry of viscous liquids and polymers Field-cycling NMR relaxometry of viscous liquids and polymers Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 27 August 2011</br> D., Kruk , A., Herrmann , E.A., Rössler</br> Graphical abstract Graphical abstract:Highlights:? NMR relaxometry compared with DQ NMR, dielectric spectroscopy and light scattering ? Applying susceptibility representation and frequency-temperature superposition ? Liquids: Intra- & intermolecular relaxation give rotational & translational correlation times ? Polymers:...	nmrlearner	Journal club	0	08-29-2011 06:41 AM
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field Abstract Magic-angle spinning (MAS) solid-state NMR (SSNMR) spectroscopy of uniformly-13C,15N labeled protein samples provides insight into atomic-resolution chemistry and structure. Data collection efficiency has advanced remarkably in the last decade; however, the study of larger proteins is still challenged by relatively low resolution in comparison to solution NMR. In this study, we present a systematic analysis of SSNMR protein spectra acquired at 11.7, 17.6 and 21.1 Tesla (1H frequencies of...	nmrlearner	Journal club	0	01-09-2011 12:46 PM
[NMR paper] High-resolution NMR structure and backbone dynamics of the Bacillus subtilis response High-resolution NMR structure and backbone dynamics of the Bacillus subtilis response regulator, Spo0F: implications for phosphorylation and molecular recognition. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles High-resolution NMR structure and backbone dynamics of the Bacillus subtilis response regulator, Spo0F: implications for phosphorylation and molecular recognition. Biochemistry. 1997 Aug 19;36(33):10015-25 Authors: Feher VA, Zapf JW, Hoch JA, Whiteley JM, McIntosh LP, Rance M, Skelton NJ,...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cy Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR. Related Articles Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR. Biochemistry. 1994 Mar 8;33(9):2459-67 Authors: Pinheiro TJ, Watts A A model of the inner mitochondrial membrane was constructed with dioleoyphosphatidylcholine (PC), dioleoylphosphatidylethanolamine...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] High-resolution 13C NMR study of the topography and dynamics of methionine residues i High-resolution 13C NMR study of the topography and dynamics of methionine residues in detergent-solubilized bacteriorhodopsin. Related Articles High-resolution 13C NMR study of the topography and dynamics of methionine residues in detergent-solubilized bacteriorhodopsin. Biochemistry. 1991 Apr 23;30(16):3885-92 Authors: Seigneuret M, Neumann JM, Levy D, Rigaud JL The proton transport membrane protein bacteriorhodopsin has been biosynthetically labeled with methionine and studied by high-resolution 13C NMR after solubilization in the detergent...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR tweet] Radiology publications: Desktop fast-field cycling nuclear magnetic resonance relaxom Radiology publications: Desktop fast-field cycling nuclear magnetic resonance relaxometer. ... http://bit.ly/dmjaCl Source: Twitter	nmrlearner	Twitter NMR	0	08-14-2010 02:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off