High-pressure is a well-known perturbation method that can be used to destabilize globular proteins and dissociate protein complexes in a reversible manner. Hydrostatic pressure drives thermodynamical equilibria toward the state(s) with the lower molar volume. Increasing pressure offers, therefore, the opportunities to finely tune the stability of globular proteins and the oligomerization equilibria of protein complexes. High-pressure NMR experiments allow a detailed characterization of the...
[NMR paper] High pressure NMR reveals conformational perturbations by disease-causing mutations in amyloid ?-peptide.
High pressure NMR reveals conformational perturbations by disease-causing mutations in amyloid ?-peptide.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles High pressure NMR reveals conformational perturbations by disease-causing mutations in amyloid ?-peptide.
Chem Commun (Camb). 2018 May 01;54(36):4609-4612
Authors: Rosenman DJ, Clemente N, Ali M, García AE, Wang C
Abstract
Here we present the high pressure NMR characterization of A?42 and two...
[NMR paper] High-Pressure NMR Spectroscopy Reveals Functional Sub-states of Ubiquitin and Ubiquitin-Like Proteins.
High-Pressure NMR Spectroscopy Reveals Functional Sub-states of Ubiquitin and Ubiquitin-Like Proteins.
Related Articles High-Pressure NMR Spectroscopy Reveals Functional Sub-states of Ubiquitin and Ubiquitin-Like Proteins.
Subcell Biochem. 2015;72:199-214
Authors: Kitahara R
Abstract
High-pressure nuclear magnetic resonance (NMR) spectroscopy has revealed that ubiquitin has at least two high-energy states - an alternatively folded state N2 and a locally disordered state I - between the basic folded state N1 and totally unfolded U...
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07-16-2015 11:21 AM
[NMR paper] Cavity as a Source of Conformational Fluctuation and High-Energy State: High-Pressure NMR Study of a Cavity-Enlarged Mutant of T4Lysozyme.
Cavity as a Source of Conformational Fluctuation and High-Energy State: High-Pressure NMR Study of a Cavity-Enlarged Mutant of T4Lysozyme.
Cavity as a Source of Conformational Fluctuation and High-Energy State: High-Pressure NMR Study of a Cavity-Enlarged Mutant of T4Lysozyme.
Biophys J. 2015 Jan 6;108(1):133-145
Authors: Maeno A, Sindhikara D, Hirata F, Otten R, Dahlquist FW, Yokoyama S, Akasaka K, Mulder FA, Kitahara R
Abstract
Although the structure, function, conformational dynamics, and controlled thermodynamics of proteins...
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01-08-2015 01:29 PM
[NMR paper] Distinct conformational states of the Alzheimer ?-amyloid peptide can be detected by high-pressure NMR spectroscopy.
Distinct conformational states of the Alzheimer ?-amyloid peptide can be detected by high-pressure NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Distinct conformational states of the Alzheimer ?-amyloid peptide can be detected by high-pressure NMR spectroscopy.
Angew Chem Int Ed Engl. 2013 Aug 19;52(34):8943-7
Authors: Munte CE, Beck Erlach M, Kremer W, Koehler J, Kalbitzer HR
PMID: 23843225
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[NMR paper] Intrinsic Allosteric Inhibition of Signaling Proteins by Targeting Rare Interaction States Detected by High-Pressure NMR Spectroscopy.
Intrinsic Allosteric Inhibition of Signaling Proteins by Targeting Rare Interaction States Detected by High-Pressure NMR Spectroscopy.
Related Articles Intrinsic Allosteric Inhibition of Signaling Proteins by Targeting Rare Interaction States Detected by High-Pressure NMR Spectroscopy.
Angew Chem Int Ed Engl. 2013 Nov 11;
Authors: Kalbitzer HR, Rosnizeck IC, Munte CE, Narayanan SP, Kropf V, Spoerner M
Abstract
A new type of allosteric inhibition by small molecules is proposed that should be applicable to all proteins involved intrinsically...
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11-13-2013 09:22 PM
[NMR paper] Detecting Intracellular Cysteine Redox States by in-Cell NMR Spectroscopy.
Detecting Intracellular Cysteine Redox States by in-Cell NMR Spectroscopy.
Related Articles Detecting Intracellular Cysteine Redox States by in-Cell NMR Spectroscopy.
Chembiochem. 2013 Jul 24;
Authors: Silvers R, Schwalbe H
Abstract
An in-cell perspective: Nowadays, in-cell NMR spectroscopy has proven to be a thrilling alternative for the investigation of biomacromolecules under physiological conditions at atomic resolution. A recent example demonstrating significant progress in in-cell NMR was published by the groups of Banci and Aricescu...
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[NMR paper] Impact of Hydrostatic Pressure on an Intrinsically Disordered Protein: A High-Pressure NMR Study of ?-Synuclein.
Impact of Hydrostatic Pressure on an Intrinsically Disordered Protein: A High-Pressure NMR Study of ?-Synuclein.
Related Articles Impact of Hydrostatic Pressure on an Intrinsically Disordered Protein: A High-Pressure NMR Study of ?-Synuclein.
Chembiochem. 2013 Jun 28;
Authors: Roche J, Ying J, Maltsev AS, Bax A
Abstract
The impact of pressure on the backbone (15) N, (1) H and (13) C chemical shifts in N-terminally acetylated ?-synuclein has been evaluated over a pressure range 1-2500 bar. Even while the chemical shifts fall very close...
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
Linear Mode
