Studying the structural aspects of proteins within sub-cellular compartments is of growing interest. Dynamic nuclear polarization supported solid-state NMR (DNP-ssNMR) is uniquely suited to provide such information, but critically lacks the desired sensitivity and resolution. Here we utilize SNAPol-1, a novel biradical, to conduct DNP-ssNMR at high-magnetic fields (800 MHz/527 GHz) inside HeLa cells and isolated cell nuclei electroporated with [^(13)C,^(15)N] labeled ubiquitin. We report that...
DNP and Cellular Solid-state NMR #DNPNMR
From The DNP-NMR Blog:
DNP and Cellular Solid-state NMR #DNPNMR
Paioni, Alessandra Lucini, Marie A M Renault, and Marc Baldus. “DNP and Cellular Solid-State NMR,” 7:12, 2018.
https://onlinelibrary.wiley.com/doi/abs/10.1002/9780470034590.emrstm1561.
Solid-state nuclear magnetic resonance (ssNMR) can provide structural information at the most detailed level and, at the same time, is applicable in highly heterogeneous and complex molecular environments, largely irrespective of solubility or crystallinity. Revolutionary developments in the field of dynamic nuclear polarization (DNP)...
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[NMR paper] Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
J Struct Biol. 2017 Nov 29;:
Authors: Pinto C, Mance D, Julien M, Daniels M, Weingarth M, Baldus M
Abstract
Significant progress has been made in obtaining a structural insight into the assembly of the ?-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane...
[NMR paper] Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling.
Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling.
Related Articles Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling.
J Biomol NMR. 2015 May 9;
Authors: Baker LA, Daniëls M, van der Cruijsen EA, Folkers GE, Baldus M
Abstract
Solid-state NMR spectroscopy (ssNMR) has made significant progress towards the study of membrane proteins in their native cellular membranes. However, reduced spectroscopic sensitivity and high background signal levels can complicate these...
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05-10-2015 07:49 PM
Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling
Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling
Abstract
Solid-state NMR spectroscopy (ssNMR) has made significant progress towards the study of membrane proteins in their native cellular membranes. However, reduced spectroscopic sensitivity and high background signal levels can complicate these experiments. Here, we describe a method for ssNMR to specifically label a single protein by repressing endogenous protein expression with rifampicin. Our results demonstrate that treatment of E. coli with rifampicin during...
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05-08-2015 09:18 PM
Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization
From The DNP-NMR Blog:
Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization
Yamamoto, K., et al., Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization. Biochimica et Biophysica Acta (BBA) - Biomembranes, 2015. 1848(1, Part B): p. 342-349.
http://dx.doi.org/10.1016/j.bbamem.2014.07.008
A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity
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