The hyperpolarization of heteronuclei via Signal Amplification by Reversible Exchange (SABRE) was investigated under conditions of heterogeneous catalysis and microtesla magnetic fields. Immobilization of [IrCl(COD)(IMes)], [IMes = 1,3-bis(2,4,6-trimethylphenyl), imidazole-2-ylidene; COD = cyclooctadiene] catalyst onto silica particles modified with NH2(CH2)3- linkers engenders an effective heterogeneous SABRE (HET-SABRE) catalyst that was used to demonstrate ~102-fold enhancement of 15N NMR signals in pyridine at 9.4 T following parahydrogen bubbling within a magnetic shield. No 15N NMR enhancement was observed from the supernatant liquid following catalyst separation, which along with XRD characterization, supports that the effects result from SABRE under heterogeneous catalytic conditions. The technique can be developed further for producing catalyst-free agents via SABRE with hyperpolarized heteronuclear spins, and thus is promising for biomedical NMR and MRI applications.
One-thousand-fold enhancement of high field liquid nuclear magnetic resonance signals at room temperature
From The DNP-NMR Blog:
One-thousand-fold enhancement of high field liquid nuclear magnetic resonance signals at room temperature
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Liu, G., et al., One-thousand-fold enhancement of high field liquid nuclear magnetic resonance signals at room temperature. Nat Chem, 2017. advance online publication.
http://dx.doi.org/10.1038/nchem.2723
nmrlearner
News from NMR blogs
0
03-17-2017 08:34 PM
Dynamic Nuclear Polarization Solid-State NMR in Heterogeneous Catalysis Research
From The DNP-NMR Blog:
Dynamic Nuclear Polarization Solid-State NMR in Heterogeneous Catalysis Research
Kobayashi, T., et al., Dynamic Nuclear Polarization Solid-State NMR in Heterogeneous Catalysis Research. ACS Catalysis, 2015. 5(12): p. 7055-7062.
http://dx.doi.org/10.1021/acscatal.5b02039
nmrlearner
News from NMR blogs
0
02-10-2016 09:28 PM
NMR Signal Enhancement by Effective SABRE Labeling of Oligopeptides
From The DNP-NMR Blog:
NMR Signal Enhancement by Effective SABRE Labeling of Oligopeptides
Ratajczyk, T., et al., NMR Signal Enhancement by Effective SABRE Labeling of Oligopeptides. Chemistry, 2015: p. n/a-n/a.
http://www.ncbi.nlm.nih.gov/pubmed/26189499
nmrlearner
News from NMR blogs
0
08-12-2015 10:04 PM
Microtesla SABRE enables 10% nitrogen-15 nuclear spin polarization
From The DNP-NMR Blog:
Microtesla SABRE enables 10% nitrogen-15 nuclear spin polarization
Theis, T., et al., Microtesla SABRE enables 10% nitrogen-15 nuclear spin polarization. J Am Chem Soc, 2015. 137(4): p. 1404-7.
http://www.ncbi.nlm.nih.gov/pubmed/25583142
nmrlearner
News from NMR blogs
0
05-07-2015 12:59 AM
[NMR paper] The Heterogeneous Structural Behavior of E7 from HPV16 Revealed by NMR Spectroscopy.
The Heterogeneous Structural Behavior of E7 from HPV16 Revealed by NMR Spectroscopy.
The Heterogeneous Structural Behavior of E7 from HPV16 Revealed by NMR Spectroscopy.
Chembiochem. 2013 Aug 12;
Authors: Calçada EO, Felli IC, Hoek T, Pierattelli R
Abstract
The E7 protein from human papillomavirus (HPV) plays a key role in oncogenesis; for this reason, it is a target of great biomedical interest. To date, no high resolution information is available for the full protein. We present here the NMR characterization of the entire E7 from...
nmrlearner
Journal club
0
08-14-2013 05:24 PM
[NMR paper] PASE (PAramagnetic signals enhancement): a new method for NMR study of paramagnetic p
PASE (PAramagnetic signals enhancement): a new method for NMR study of paramagnetic proteins.
Related Articles PASE (PAramagnetic signals enhancement): a new method for NMR study of paramagnetic proteins.
J Magn Reson. 1998 Sep;134(1):154-7
Authors: Bondon A, Mouro C
A new method for NMR spectra acquisition of paramagnetic proteins is described, based on the simple use of homonuclear broadband decoupling of the diamagnetic region. Several advantages are associated with this method which was applied to one-dimensional spectra, to 1D...
nmrlearner
Journal club
0
11-17-2010 11:15 PM
[NMR paper] Composition and sequence specific resonance assignments of the heterogeneous N-linked
Composition and sequence specific resonance assignments of the heterogeneous N-linked glycan in the 13.6 kDa adhesion domain of human CD2 as determined by NMR on the intact glycoprotein.
Related Articles Composition and sequence specific resonance assignments of the heterogeneous N-linked glycan in the 13.6 kDa adhesion domain of human CD2 as determined by NMR on the intact glycoprotein.
Biochemistry. 1995 Feb 7;34(5):1622-34
Authors: Wyss DF, Choi JS, Wagner G
CD2, a T cell specific surface adhesion receptor, is critically important for T...
Heterogeneous Microtesla SABRE Enhancement of 15N NMR Signals
Linear Mode
